{"code":"SASDFU7","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering.","publication":{"title":"The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering.","author_list":"Kukkurainen S, Azizi L, Zhang P, Jacquier MC, Baikoghli M, von Essen M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orłowski A, Jänis J, Määttä JAE, Varjosalo M, Vattulainen I, Róg T, Svergun D, Cheng RH, Wu J, Hytönen VP, Wehrle-Haller B","journal":"J Cell Sci","doi":"10.1242/jcs.239202","pmid":"33046605","published_date":"2020 Oct 12"},"status":"released","submitted_date":"2019-06-23","released_date":"2020-09-22"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFU7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFU7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFU7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFU7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFU7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFU7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFU7.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray 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mM sodium phosphate, 150mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.2,"deuteration":null},"purity_method":null,"name":"Talin-1 head amino acids 1-405(Δ134-170/GAG insert)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Tampere, Finland","full_name":"Tampere University","webpage":"https://www.tuni.fi/en"}],"contributor_name":"Sampo","contributor_surname":"Kukkurainen","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2015-09-24","storage_temperature":4.0,"cell_temperature":10.0,"exposure_time":0.995,"number_of_frames":20,"wavelength":0.12399,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":8.17,"sample_volume":100.0,"flow_rate":0.25,"s_min":0.025,"s_max":4.812,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFU7_fit1_model1_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDFU7_fit1_model1.pdb","model_mw":46.61,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/Ranchweb_KU6zfSB.log","symmetry":"P1","comment":null,"user":455},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFU7_fit1_model2_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":"2.1 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In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 8.2 mg/ml was injected at a 0.25 ml/min flow rate onto a column. 20 successive 0.995 second frames were collected through the sample peak (from a total of 2400) at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":46.958,"experimental_mw_error":null,"guinier_i0_mw":59.399,"guinier_i0_mw_error":null,"porod_mw":44.0,"porod_mw_error":null,"pddf_i0":3306.0,"pddf_i0_error":null,"guinier_i0":3287.85,"guinier_i0_error":16.9,"pddf_rg":3.097,"pddf_rg_error":null,"guinier_rg":3.02,"guinier_rg_error":0.08,"pddf_dmax":10.19,"pddf_dmax_error":null,"porod_volume":70.5,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":61,"guinier_point_last":145,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFU7_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}