{"code":"SASDFX3","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex.","publication":{"title":"A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex.","author_list":"Marabelli C, Marrocco B, Pilotto S, Chittori S, Picaud S, Marchese S, Ciossani G, Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A","journal":"Cell Rep","doi":"10.1016/j.celrep.2019.03.061","pmid":"30970244","published_date":"2019 Apr 9"},"status":"released","submitted_date":"2019-03-12","released_date":"2019-04-16"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFX3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFX3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFX3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFX3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFX3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFX3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFX3.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray 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Pavia","webpage":"http://wcm-3.unipv.it"}],"contributor_name":"Federico","contributor_surname":"Forneris","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2017-12-11","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":243,"wavelength":0.1,"sample_detector_distance":2.87,"concentration_min":null,"concentration_max":1.8,"sample_volume":null,"flow_rate":null,"s_min":0.071,"s_max":2.852,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of a semi-synthetic nucleosome core particle (NCP) in 15 mM HEPES, 200 mM NaCl, pH 7.3 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20 °C using the following parameters: Column: Wyatt WTC-030N5; Flow rate: 0.25 mL/min; Sample injection concentration: 2.5 mg/mL; Injection volume: 50 μL. The data were collected through the SEC peak of the sample as a series of 243 x 1 second exposures. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":200.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":90.55,"pddf_i0_error":null,"guinier_i0":91.26,"guinier_i0_error":0.1,"pddf_rg":4.268,"pddf_rg_error":null,"guinier_rg":4.35,"guinier_rg_error":0.01,"pddf_dmax":12.8,"pddf_dmax_error":null,"porod_volume":353.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":15,"guinier_point_last":56,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFX3_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}