{"code":"SASDFY8","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Therapeutic approaches to ApoE","publication":{"title":"Therapeutic approaches to ApoE","author_list":"Lucas Kraft","journal":"University of Sussex PhD thesis","doi":null,"pmid":null,"published_date":"2019 April 25"},"status":"released","submitted_date":"2019-10-25","released_date":"2021-09-27"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFY8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFY8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFY8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFY8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFY8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFY8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFY8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":"1 x 5 mm","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Apolipoprotein E2","short_name":"ApoE2","sequence":"GPHMKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKCLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH","organism":"Homo sapiens","uniprot_code":"P02649","uniprot_range_first":19,"uniprot_range_last":317,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":"MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQT\nLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQA\nRLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVY\nQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMG\nSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEK\nVQAAVGTSAAPVPSDNH","mw":34.606,"total_mw":138.848,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Heparin","short_name":null,"sequence":"(C12H19NO20S3)n","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"other","uniprot_sequence":null,"mw":15.0,"total_mw":15.0,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM HEPES, 300 mM NaCl, 1 mM TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Apolipoprotein E2 (ApoE2) bound to 110 µg/mL heparin","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Brighton, UK","full_name":"University of Sussex","webpage":"http://www.sussex.ac.uk/"}],"contributor_name":"Lucas","contributor_surname":"Kraft","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2018-05-03","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":28,"wavelength":0.1,"sample_detector_distance":4.036,"concentration_min":null,"concentration_max":4.0,"sample_volume":null,"flow_rate":null,"s_min":0.03,"s_max":3.7,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5","i0_calibration_standard":null,"description":"ApoE2 + 110 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.","experiment_description":"Synchrotron SAXS data from solutions of ApoE2 bound to 110 µg/mL heparin in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 20°C. 28 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":259.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.6547,"pddf_i0_error":null,"guinier_i0":0.659,"guinier_i0_error":0.0045,"pddf_rg":7.16,"pddf_rg_error":null,"guinier_rg":7.061,"guinier_rg_error":0.073,"pddf_dmax":24.0,"pddf_dmax_error":null,"porod_volume":666.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":23,"guinier_point_last":68,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFY8_datloglog_img.png","symmetry":null,"last_modified":"2024-10-07T16:05:04.128218+02:00","bragg_peak":[]}