{"code":"SASDG53","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"The guide sRNA sequence determines the activity level of box C/D RNPs.","publication":{"title":"The guide sRNA sequence determines the activity level of box C/D RNPs.","author_list":"Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T","journal":"Elife","doi":"10.7554/eLife.50027","pmid":"32202498","published_date":"2020 Mar 23"},"status":"released","submitted_date":"2019-07-29","released_date":"2020-03-25"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDG53.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDG53.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG53_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG53_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDG53_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG53_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDG53.sascif","experiment":{"instrument":{"detector":{"type":"128 linear sensitive multidetector","name":"Reuter-Stokes 3He","resolution":0.8},"name":"Institut Laue-Langevin (ILL)","city":"Grenoble","country":"France","beamline_name":"D22","beam_geometry":null,"type_of_source":"neutron source","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase","short_name":"Fibrillarin","sequence":">sp|Q8U4M2|FLPA_PYRFU Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=flpA PE=1 SV=1\r\nMVEVKKHKFPGVYVVIDDDGSEKIATKNLVPGQRVYGERVIKWEGEEYRIWNPHRSKLGA\r\nAIVNGLKNFPIKPGKSVLYLGIASGTTASHVSDIVGWEGKIYGIEFSPRVLRELVPIVEE\r\nRRNIIPILGDATKPEEYRALVTKVDVIFEDVAQPTQAKILIDNAKAYLKRGGYGMIAVKS\r\nRSIDVTKEPEQVFKEVERELSEYFEVIERLNLEPYEKDHALFVVRKP","organism":"Pyrococcus 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SV=1\r\nMKAFISENVRGIYAFDENGNLIEKRYFTDKPEKVLDQLLKGEITKDLEELLNSLKEKGYD\r\nEFVFEHPELSRRAKELGFSATTEFPNIAGERLRSNPEEFLGENWFEEYYKVGVALTRMRI\r\nQEQSGARDKMVIQAIEALDDVDKVINLLVARLREWYSLHFPELDELLPKHPQYVAFVKTV\r\nGHRDNINEEVLRELGLSEEKIKKILEAKEKTMGAWMDQTDIEVVRQLAEEIDRLYQLRKK\r\nLEDYIDRAMDDVAPNLKALVGAKLAARLISLAGGLRELAMMPSSTIQVLGAEKALFRHLR\r\nTGAKPPKHGVIYQYPAINRSPWWQRGKIARALAGKLAIAARVDYFSGEYIAEELKKELEA\r\nRIREIKEKYPKPPKRKREERKGKKPWKKDKKKKKKKEKPRRR","organism":"Pyrococcus 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furiosus","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"RNA","uniprot_sequence":null,"mw":23.548,"total_mw":23.548,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Pyrococcus furiosus sR26 substrate D","short_name":"Substrate D","sequence":">Substrate D\r\nGUAGAUCACCUCCG","organism":"Pyrococcus furiosus","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"RNA","uniprot_sequence":null,"mw":4.486,"total_mw":4.486,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM phosphate 500 mM NaCl 42%D2O","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.6,"deuteration":null},"purity_method":null,"name":"The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D and 2H Nop5 (SANS data in 42% v/v D2O)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Germany","full_name":"Leibniz University of Hannover","webpage":"https://www.uni-hannover.de"}],"contributor_name":"Andrea","contributor_surname":"Graziadei","orcid":"https://orcid.org/0000-0001-7709-6002"}],"concentration_method":null,"concentration_unit":null,"date":"2015-09-21","storage_temperature":24.0,"cell_temperature":55.0,"exposure_time":900.0,"number_of_frames":1,"wavelength":0.6,"sample_detector_distance":4.0,"concentration_min":null,"concentration_max":5.4,"sample_volume":null,"flow_rate":null,"s_min":0.128,"s_max":2.388,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":null,"experiment_description":"SANS data from solutions of the Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D and 2H Nop5 in 50 mM phosphate 500 mM NaCl 42%D2O, pH 6.6 were collected on D22 (ILL; Grenoble, France) using a 128 linear sensitive Reuter-Stokes detector detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.6 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.40 mg/ml was measured at 55°C. One 900 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":200.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.3353,"pddf_i0_error":null,"guinier_i0":0.338,"guinier_i0_error":0.0017,"pddf_rg":4.1,"pddf_rg_error":null,"guinier_rg":4.125,"guinier_rg_error":0.068,"pddf_dmax":14.0,"pddf_dmax_error":null,"porod_volume":222.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":9,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDG53_datloglog_img.png","symmetry":null,"last_modified":"2022-08-15T14:51:04.900446+02:00","bragg_peak":[]}