{"code":"SASDG55","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states","publication":{"title":"Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states","author_list":"Stachowski T, Snell M, Snell E","journal":"IUCrJ","doi":"10.1107/S205225251901707X","pmid":null,"published_date":"2020 Feb 06"},"status":"released","submitted_date":"2019-09-04","released_date":"2020-02-28"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDG55.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDG55.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG55_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG55_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDG55_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG55_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDG55.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 2M","resolution":172.0},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Latency associated peptide","short_name":"LAP","sequence":">sp|P01137|30-278\r\nLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVA\r\nGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVL\r\nLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLS\r\nRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQ\r\nHLQSSRHRAHHHHHH","organism":"Homo sapiens","uniprot_code":"P01137","uniprot_range_first":30,"uniprot_range_last":277,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MPPSGLRLLLLLLPLLWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLA\nSPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEI\nYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWR\nYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFT\nTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYI\nDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQA\nLEPLPIVYYVGRKPKVEQLSNMIVRSCKCS","mw":29.228,"total_mw":58.456,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"phosphate buffered saline 2% glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Deglycosylated latency associated peptide, LAP (TGFB-1)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"New York, USA","full_name":"Hauptman-Woodward Medical Research Institute","webpage":"http://hwi.buffalo.edu/"}],"contributor_name":"Tim","contributor_surname":"Stachowski","orcid":"https://orcid.org/0000-0002-6097-4857"}],"concentration_method":null,"concentration_unit":null,"date":"2019-04-20","storage_temperature":4.0,"cell_temperature":10.0,"exposure_time":0.1,"number_of_frames":100,"wavelength":0.127,"sample_detector_distance":2.0,"concentration_min":null,"concentration_max":1.4,"sample_volume":null,"flow_rate":null,"s_min":0.109,"s_max":2.611,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of deglycosylated latency associated peptide (LAP) from the transforming growth factor beta-1 proprotein (TGFB-1) in phosphate buffered saline, 2% glycerol, pH 7.4 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.40 mg/ml was measured at 10°C. 100 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":59.1,"experimental_mw_error":null,"guinier_i0_mw":47.0,"guinier_i0_mw_error":5.0,"porod_mw":null,"porod_mw_error":null,"pddf_i0":1.987,"pddf_i0_error":null,"guinier_i0":1.99,"guinier_i0_error":0.01,"pddf_rg":3.55,"pddf_rg_error":null,"guinier_rg":3.51,"guinier_rg_error":0.11,"pddf_dmax":13.0,"pddf_dmax_error":null,"porod_volume":129.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":null,"guinier_point_last":null,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDG55_datloglog_img.png","symmetry":null,"last_modified":"2020-02-28T18:01:03.158567+01:00","bragg_peak":[]}