{"code":"SASDGA3","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"The guide sRNA sequence determines the activity level of box C/D RNPs.","publication":{"title":"The guide sRNA sequence determines the activity level of box C/D RNPs.","author_list":"Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T","journal":"Elife","doi":"10.7554/eLife.50027","pmid":"32202498","published_date":"2020 Mar 23"},"status":"released","submitted_date":"2019-07-29","released_date":"2020-03-25"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDGA3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDGA3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGA3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGA3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDGA3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGA3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDGA3.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase","short_name":"Fibrillarin","sequence":">sp|Q8U4M2|FLPA_PYRFU Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=flpA PE=1 SV=1\r\nMVEVKKHKFPGVYVVIDDDGSEKIATKNLVPGQRVYGERVIKWEGEEYRIWNPHRSKLGA\r\nAIVNGLKNFPIKPGKSVLYLGIASGTTASHVSDIVGWEGKIYGIEFSPRVLRELVPIVEE\r\nRRNIIPILGDATKPEEYRALVTKVDVIFEDVAQPTQAKILIDNAKAYLKRGGYGMIAVKS\r\nRSIDVTKEPEQVFKEVERELSEYFEVIERLNLEPYEKDHALFVVRKP","organism":"Pyrococcus 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SV=1\r\nMKAFISENVRGIYAFDENGNLIEKRYFTDKPEKVLDQLLKGEITKDLEELLNSLKEKGYD\r\nEFVFEHPELSRRAKELGFSATTEFPNIAGERLRSNPEEFLGENWFEEYYKVGVALTRMRI\r\nQEQSGARDKMVIQAIEALDDVDKVINLLVARLREWYSLHFPELDELLPKHPQYVAFVKTV\r\nGHRDNINEEVLRELGLSEEKIKKILEAKEKTMGAWMDQTDIEVVRQLAEEIDRLYQLRKK\r\nLEDYIDRAMDDVAPNLKALVGAKLAARLISLAGGLRELAMMPSSTIQVLGAEKALFRHLR\r\nTGAKPPKHGVIYQYPAINRSPWWQRGKIARALAGKLAIAARVDYFSGEYIAEELKKELEA\r\nRIREIKEKYPKPPKRKREERKGKKPWKKDKKKKKKKEKPRRR","organism":"Pyrococcus 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furiosus","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"RNA","uniprot_sequence":null,"mw":23.548,"total_mw":23.548,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Pyrococcus furiosus sR26 substrate D","short_name":"Substrate D","sequence":">Substrate D\r\nGUAGAUCACCUCCG","organism":"Pyrococcus furiosus","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"RNA","uniprot_sequence":null,"mw":4.486,"total_mw":4.486,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM phosphate 500 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.6,"deuteration":null},"purity_method":null,"name":"Pyrococcus furiosus Box C/D sRNP reconstituted with st-sR6 sRNA bound to substrate D (SAXS data)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Germany","full_name":"Leibniz University of Hannover","webpage":"https://www.uni-hannover.de"}],"contributor_name":"Andrea","contributor_surname":"Graziadei","orcid":"https://orcid.org/0000-0001-7709-6002"}],"concentration_method":null,"concentration_unit":null,"date":"2015-07-13","storage_temperature":20.0,"cell_temperature":40.1,"exposure_time":1.0,"number_of_frames":10,"wavelength":0.09919,"sample_detector_distance":2.864,"concentration_min":null,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.033,"s_max":4.934,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Pyrococcus furiosus Box C/D sRNP reconstituted with st-sR6 sRNA bound to substrate D in 50 mM phosphate 500 mM NaCl, pH 6.6 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 40.1°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":214.0,"experimental_mw_error":null,"guinier_i0_mw":214.9,"guinier_i0_mw_error":0.1,"porod_mw":null,"porod_mw_error":null,"pddf_i0":214.5,"pddf_i0_error":null,"guinier_i0":214.87,"guinier_i0_error":0.1,"pddf_rg":5.0,"pddf_rg_error":null,"guinier_rg":5.0,"guinier_rg_error":0.01,"pddf_dmax":17.0,"pddf_dmax_error":null,"porod_volume":429.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":16,"guinier_point_last":49,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDGA3_datloglog_img.png","symmetry":null,"last_modified":"2020-03-25T10:04:13.882830+01:00","bragg_peak":[]}