{"code":"SASDGB6","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Large-scale conformational rearrangement of the α5-helix of Gα subunits in complex with the guanine nucleotide exchange factor Ric8A.","publication":{"title":"Large-scale conformational rearrangement of the α5-helix of Gα subunits in complex with the guanine nucleotide exchange factor Ric8A.","author_list":"Srivastava D, Artemyev NO","journal":"J Biol Chem","doi":"10.1074/jbc.AC119.011135","pmid":"31624147","published_date":"2019 Oct 17"},"status":"released","submitted_date":"2019-09-26","released_date":"2019-11-04"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDGB6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDGB6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGB6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGB6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDGB6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGB6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDGB6.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 1M","resolution":0.172},"name":"Advanced Photon Source (APS), Argonne National Laboratory","city":"Lemont, IL","country":"USA","beamline_name":"BioCAT 18ID","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Resistance to inhibitors of cholinesterase 8 homolog A","short_name":"Ric8a","sequence":"GHMADPRAVADALETGEEDVVMEALRAYNRENSQSFTFDDAQQEDRKRLAKLLVSVLEQGLPP\r\nSRRVIWLQSIRILSRDRSCLDSFTSRRSLQALACYAGISASQGSVPEPLNMDVVLESLKC\r\nLCNLVLSSPVAQALAAEAGLVVRLAERVGLCRQSSFPHDVQFFDLRLLFLLTALRTDVRQ\r\nQLFQELQGVRLLTRALELTLGMTEGERHPELLPPQETERAMEILKVLFNITFDSIKREVD\r\nEEDAALYRHLGTLLRHCVMLAAAGDRTEELHGHAVNLLGNLPVKCLDVLLTLEPHEGSLE\r\nFLGVNMDVIRVLLSFMEKRLHQTHRLKESVAPVLSVLTECARMHRPARKFLKAQVLPPLR\r\nDVRTRPEVGELLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLA\r\nARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAM\r\nKLVNMFDKLSRH","organism":"Bos taurus","uniprot_code":"Q5E9J8","uniprot_range_first":1,"uniprot_range_last":492,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MEPRAVADALETGEEDVVMEALRAYNRENSQSFTFDDAQQEDRKRLAKLLVSVLEQGLPP\nSRRVIWLQSIRILSRDRSCLDSFTSRRSLQALACYAGISASQGSVPEPLNMDVVLESLKC\nLCNLVLSSPVAQALAAEAGLVVRLAERVGLCRQSSFPHDVQFFDLRLLFLLTALRTDVRQ\nQLFQELQGVRLLTRALELTLGMTEGERHPELLPPQETERAMEILKVLFNITFDSIKREVD\nEEDAALYRHLGTLLRHCVMLAAAGDRTEELHGHAVNLLGNLPVKCLDVLLTLEPHEGSLE\nFLGVNMDVIRVLLSFMEKRLHQTHRLKESVAPVLSVLTECARMHRPARKFLKAQVLPPLR\nDVRTRPEVGELLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLA\nARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAM\nKLVNMFDKLSRHRVIQPMGMSPRGQLTSLQDAMCETMEGQLSSDPDSDPD","mw":55.643,"total_mw":55.643,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"miniGi","short_name":null,"sequence":"AMEKAAREVKLLLLGADNSGKSTIVKQMKIIHEAGEYMPMERVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEDVAAIIFCVDLSDYEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYQEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF","organism":"synthetic construct","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":null,"mw":24.54,"total_mw":24.54,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris, 150 mM KCl, 5 % glycerol, 1 mM TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Resistance to inhibitors of cholinesterase 8 homolog A (Ric8A) miniGi complex","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Iowa, USA","full_name":"University of Iowa","webpage":"https://uiowa.edu/"}],"contributor_name":"Dhiraj","contributor_surname":"Srivastava","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2018-10-27","storage_temperature":4.0,"cell_temperature":null,"exposure_time":0.5,"number_of_frames":null,"wavelength":null,"sample_detector_distance":null,"concentration_min":null,"concentration_max":10.0,"sample_volume":250.0,"flow_rate":0.9,"s_min":0.039,"s_max":3.813,"total_exposure_time":null,"seccolumn":1},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDGB6_fit1_model1_img.png","software":"Other","pdb_link":[{"pdb_code":"6n85","ext_code":"6N85","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDGB6_fit1_model1.pdb","model_mw":null,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":431}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/86900_fixed_fit_img.png","software":"CRYSOL","chi_square_value":1.637,"p_value":0.0,"fit_residual_plot":"86900_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDGB6_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/86900.log","software_version":null,"description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDGB6_fit2_model1_img.png","software":"Rosetta","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":"Rosetta","model_data":"https://www.sasbdb.org/media/pdb_file/SASDGB6_fit2_model1.pdb","model_mw":79.71,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":431}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/73700_fixed_fit_img.png","software":"CRYSOL","chi_square_value":1.882,"p_value":0.0,"fit_residual_plot":"73700_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDGB6_fit2.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/73700.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":"Purified Ric8a1-492 was mixed with excess of purified miniGi and the complex was purified by size-exclusion chromatography (SEC). The complex fractions were pooled together, concentrated and additional SEC-SAXS data was collected as described above. X-ray wavelength = UNKNOWN.","experiment_description":"Synchrotron SAXS data from solutions of the Ric8A-miniGi complex in 20 mM Tris, 150 mM KCl, 5 % glycerol, 1 mM TCEP, pH 8 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 10 mg/ml was injected at a 0.90 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":83.6,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.00261,"pddf_i0_error":1.93e-06,"guinier_i0":0.0026,"guinier_i0_error":2.65e-06,"pddf_rg":3.28,"pddf_rg_error":0.003,"guinier_rg":3.24,"guinier_rg_error":0.006,"pddf_dmax":10.7,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":13,"guinier_point_last":118,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDGB6_datloglog_img.png","symmetry":null,"last_modified":"2021-01-04T14:24:46.462986+01:00","bragg_peak":[]}