{"code":"SASDGD6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"The structural basis of fungal glucuronoyl esterase activity on natural substrates.","publication":{"title":"The structural basis of fungal glucuronoyl esterase activity on natural substrates.","author_list":"Ernst HA, Mosbech C, Langkilde AE, Westh P, Meyer AS, Agger JW, Larsen S","journal":"Nat Commun","doi":"10.1038/s41467-020-14833-9","pmid":"32094331","published_date":"2020 Feb 24"},"status":"released","submitted_date":"2019-09-27","released_date":"2020-02-28"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDGD6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDGD6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGD6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGD6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDGD6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGD6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDGD6.sascif","experiment":{"instrument":{"detector":{"type":"pixel counting","name":"Pilatus3 R 300K","resolution":172.0},"name":"University of Copenhagen, Department of Drug Design and Pharmacology","city":"Copenhagen","country":"Denmark","beamline_name":"Xenocs BioXolver L with GeniX3D","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase, truncated)","short_name":"CuGE","sequence":">dDeltaS270A/95-474 + extra N-term seq from plasmid\r\nEAEAEFGACGAIASTVPNYNNAKLPDPFTFANGTALRTKADWSCRRAEISALIQNYEAGTLPPKPPVVTASF\r\nSKSGNTGTLAITAGLSNSQTIKFSPTISYPSGTPPANGWPLIIAYEGGSIPIPAGVATLTYSNSDMAQQNSA\r\nSSRGQGLFYQLYGSTHSASAMTAWVWGVSRIIDALEMTPTAQINTQRIGVTGCARDGKGALMAGAFEERIAL\r\nTIPQESGSGGDACWRLSKYEIDNGNQVQDAVEIVGENVWFSTNFNNYVQKLPTVPEDHHLLAAMVAPRAMIS\r\nFENTDYLWLSPMSSFGCMTAAHTVWQGLGIADSHGFAQVGGHAHCAWPSSLTPQLNAFINRFLLDQSATTNV\r\nFTTNNQFGKVQWNAANWITWTTPTLTENLYFQGVDHHHHHH","organism":"Cerrena unicolor","uniprot_code":"A0A0A7EQR3","uniprot_range_first":95,"uniprot_range_last":474,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MFKPSFVALALVSYATAQASAPQWGQCGGIGWTGPTACPSGWACQQLNAYYSQCLQGAAP\nAPARTTAAPPPPPATTAAPPPPTTSAPTGSSPVAGACGAIASTVPNYNNAKLPDPFTFAN\nGTALRTKADWSCRRAEISALIQNYEAGTLPPKPPVVTASFSKSGNTGTLAITAGLSNSQT\nIKFSPTISYPSGTPPANGWPLIIAYEGGSIPIPAGVATLTYSNSDMAQQNSASSRGQGLF\nYQLYGSTHSASAMTAWVWGVSRIIDALEMTPTAQINTQRIGVTGCSRDGKGALMAGAFEE\nRIALTIPQESGSGGDACWRLSKYEIDNGNQVQDAVEIVGENVWFSTNFNNYVQKLPTVPE\nDHHLLAAMVAPRAMISFENTDYLWLSPMSSFGCMTAAHTVWQGLGIADSHGFAQVGGHAH\nCAWPSSLTPQLNAFINRFLLDQSATTNVFTTNNQFGKVQWNAANWITWTTPTLT","mw":43.178,"total_mw":43.178,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM sodium acetate","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":5.0,"deuteration":null},"purity_method":null,"name":"Glucuronoyl esterase S286A, deglycolsylated truncated (amino acids 95-474)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Copenhagen, Denmark","full_name":"University of Copenhagen","webpage":"http://www.ku.dk/"}],"contributor_name":"Annette","contributor_surname":"Langkilde","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2018-10-10","storage_temperature":21.0,"cell_temperature":21.0,"exposure_time":60.0,"number_of_frames":10,"wavelength":0.154,"sample_detector_distance":0.571,"concentration_min":null,"concentration_max":3.6,"sample_volume":null,"flow_rate":null,"s_min":0.197,"s_max":4.805,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDGD6_fit1_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"6rtv","ext_code":"6RTV","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDGD6_fit1_model1.pdb","model_mw":null,"bead_radius":null,"log":null,"symmetry":"P1","comment":null,"user":42}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDGD6_fit1_fixed_fit_img.png","software":"CRYSOL","chi_square_value":1.054,"p_value":0.011142,"fit_residual_plot":"SASDGD6_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDGD6_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/dD_A.log","software_version":"2.8.3","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of N-terminal truncated mutant glucuronoyl esterase (S286A, deglycolsylated) in 20 mM sodium acetate, pH 5 were collected using a Xenocs BioXolver L with GeniX3D (University of Copenhagen, Department of Drug Design and Pharmacology) equipped with a Pilatus3 R 300K detector at a sample-detector distance of 0.6 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.60 mg/ml was measured at 21°C. 10 successive 60 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":35.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":31.0,"porod_mw_error":null,"pddf_i0":0.03734,"pddf_i0_error":null,"guinier_i0":0.037,"guinier_i0_error":null,"pddf_rg":2.05,"pddf_rg_error":null,"guinier_rg":2.01,"guinier_rg_error":0.08,"pddf_dmax":6.06,"pddf_dmax_error":null,"porod_volume":49.5,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":3,"guinier_point_last":37,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDGD6_datloglog_img.png","symmetry":null,"last_modified":"2020-11-03T10:09:23.500227+01:00","bragg_peak":[]}