{"code":"SASDGF2","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery","publication":{"title":"Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery","author_list":"Nasta V, Da Vela S, Gourdoupis S, Ciofi-Baffoni S, Svergun D, Banci L","journal":"Scientific Reports","doi":"10.1038/s41598-019-55313-5","pmid":null,"published_date":"2019 Dec 12"},"status":"released","submitted_date":"2019-07-22","released_date":"2019-12-07"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDGF2.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDGF2.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGF2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGF2_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDGF2_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGF2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDGF2.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 6M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Putative transferase CAF17, mitochondrial","short_name":"IBA57","sequence":"AGAAWACFRLDGRTLLRVRGPDAAPFLLGLLTNELPLPSPAAAGAPPAARAGYAHFLNVQGRTLYDVILYGLQEHSEVSGFLLECDSSVQGALQKHLALYRIRRKVTVEPHPELRVWAVLPSSPEACGAASLQERAGAAAILIRDPRTARMGWRLLTQDEGPALVPGGRLGDLWDYHQHRYLQGVPEGVRDLPPGVALPLESNLAFMNGVSFTKGCYIGQELTARTHHMGVIRKRLFPVRFLDPLPTSGITPGATVLTASGQTVGKFRAGQGNVGLALLWSEKIKGPLHIRASEGAQVALAASVPDWWP","organism":"Homo sapiens","uniprot_code":"Q5T440","uniprot_range_first":44,"uniprot_range_last":352,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MATAALLRGATPGRGGPVWRWRLRAAPRCRLAHSSCSPGGDPTAGAAWACFRLDGRTLLR\nVRGPDAAPFLLGLLTNELPLPSPAAAGAPPAARAGYAHFLNVQGRTLYDVILYGLQEHSE\nVSGFLLECDSSVQGALQKHLALYRIRRKVTVEPHPELRVWAVLPSSPEACGAASLQERAG\nAAAILIRDPRTARMGWRLLTQDEGPALVPGGRLGDLWDYHQHRYLQGVPEGVRDLPPGVA\nLPLESNLAFMNGVSFTKGCYIGQELTARTHHMGVIRKRLFPVRFLDPLPTSGITPGATVL\nTASGQTVGKFRAGQGNVGLALLWSEKIKGPLHIRASEGAQVALAASVPDWWPTVSK","mw":33.242,"total_mw":33.242,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Iron-sulfur cluster assembly 2 homolog, mitochondrial","short_name":"ISCA2","sequence":"EAGEGQIRLTDSCVQRLLEITEGSEFLRLQVEGGGCSGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGCSCGSSFSIKL","organism":"Homo sapiens","uniprot_code":"Q86U28","uniprot_range_first":44,"uniprot_range_last":154,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MAAAWGSSLTAATQRAVTPWPRGRLLTASLGPQARREASSSSPEAGEGQIRLTDSCVQRL\nLEITEGSEFLRLQVEGGGCSGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQ\nVDFSQELIRSSFQVLNNPQAQQGCSCGSSFSIKL","mw":12.041,"total_mw":24.082,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM phosphate, 150 mM NaCl, 5 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Putative mitochondrial transferase CAF17 bound to mitochondrial iron-sulfur cluster assembly ISCA2 protein (human IBA57-ISCA2 complex)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Stefano","contributor_surname":"Da Vela","orcid":"https://orcid.org/0000-0002-1700-4759"}],"concentration_method":null,"concentration_unit":null,"date":"2018-11-22","storage_temperature":10.0,"cell_temperature":20.0,"exposure_time":0.095,"number_of_frames":40,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":0.9,"sample_volume":null,"flow_rate":null,"s_min":0.086,"s_max":2.998,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDGF2_fit1_model1_img.png","software":"SASREF CV","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDGF2_fit1_model1.pdb","model_mw":90.7,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":471}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDGF2_fit1_fixed_fit_img.png","software":"SASREF CV","chi_square_value":1.365,"p_value":0.0,"fit_residual_plot":"SASDGF2_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDGF2_fit1.fit","fit_log":null,"software_version":null,"description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDGF2_fit2_model1_img.png","software":"MONSA","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDGF2_fit2_model1.pdb","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":null,"comment":"Monsa model (together with the ISCA2, see entry SASDGE2)","user":471}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDGF2_fit2_fixed_fit_img.png","software":"MONSA","chi_square_value":1.197,"p_value":0.0258,"fit_residual_plot":"SASDGF2_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDGF2_fit2.fit","fit_log":null,"software_version":null,"description":"Monsa model (together with the ISCA2, see entry SASDGE2)"}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the human IBA57-ISCA2 protein complex in 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.90 mg/ml was measured at 20°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":76.35,"experimental_mw_error":null,"guinier_i0_mw":79.9,"guinier_i0_mw_error":null,"porod_mw":71.0,"porod_mw_error":null,"pddf_i0":0.06335,"pddf_i0_error":null,"guinier_i0":0.06197,"guinier_i0_error":0.00025,"pddf_rg":4.313,"pddf_rg_error":null,"guinier_rg":3.95,"guinier_rg_error":0.12,"pddf_dmax":16.38,"pddf_dmax_error":null,"porod_volume":114.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":4,"guinier_point_last":89,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDGF2_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:55:24.740692+02:00","bragg_peak":[]}