{"code":"SASDGJ2","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"The structure of the extracellular domains of human interleukin 11 α-receptor reveals mechanisms of cytokine engagement","publication":{"title":"The structure of the extracellular domains of human interleukin 11 α-receptor reveals mechanisms of cytokine engagement","author_list":"Metcalfe R, Aizel K, Zlatic C, Nguyen P, Morton C, Lio D, Cheng H, Dobson R, Parker M, Gooley P, Putoczki T, Griffin M","journal":"Journal of Biological Chemistry","doi":"10.1074/jbc.RA119.012351","pmid":null,"published_date":"2020 Apr 24"},"status":"released","submitted_date":"2019-07-24","released_date":"2020-04-25"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDGJ2.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDGJ2.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGJ2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGJ2_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDGJ2_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDGJ2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDGJ2.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 S 2M","resolution":172.0},"name":"Australian Synchrotron","city":"Melbourne","country":"Australia","beamline_name":"SAXS/WAXS","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Interleukin 11","short_name":"IL11","sequence":"GPGPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSAGALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL","organism":"Homo 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University of Melbourne","webpage":"http://www.bio21.unimelb.edu.au/"}],"contributor_name":"Riley D.","contributor_surname":"Metcalfe","orcid":"https://orcid.org/0000-0002-7154-780X"}],"concentration_method":null,"concentration_unit":null,"date":"2019-06-08","storage_temperature":null,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":11,"wavelength":0.1078,"sample_detector_distance":2.038,"concentration_min":null,"concentration_max":5.0,"sample_volume":50.0,"flow_rate":0.45,"s_min":0.074,"s_max":6.639,"total_exposure_time":null,"seccolumn":2},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDGJ2_fit1_model1_img.png","software":"PDB (Protein Data 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Tris, 150 mM NaCl, 0.2% sodium azide, pH 8.5 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Dectris/Pilatus3 S 2M detector at a sample-detector distance of 2.0 m and at a wavelength of λ = 0.1078 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 5 mg/ml was injected at a 0.45 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 20°C. 11 successive 1 second frames were collected through the SEC elution peak of the sample. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":17.2,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":17.0,"porod_mw_error":null,"pddf_i0":0.006783,"pddf_i0_error":null,"guinier_i0":0.006792,"guinier_i0_error":2.4e-05,"pddf_rg":1.9,"pddf_rg_error":null,"guinier_rg":1.889,"guinier_rg_error":0.011,"pddf_dmax":6.1,"pddf_dmax_error":null,"porod_volume":27.6,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":5,"guinier_point_last":64,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDGJ2_datloglog_img.png","symmetry":null,"last_modified":"2020-04-25T09:37:21.313598+02:00","bragg_peak":[]}