{"code":"SASDUU8","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Selective deuteration of an RNA:RNA complex for structural analysis using small-angle scattering.","publication":{"title":"Selective deuteration of an RNA:RNA complex for structural analysis using small-angle scattering.","author_list":"Munsayac A, Leite WC, Hopkins JB, Hall I, O'Neill HM, Keane SC","journal":"Structure","doi":"10.1016/j.str.2025.01.017","pmid":"39933513","published_date":"2025 Feb 6"},"status":"released","submitted_date":"2024-05-16","released_date":"2025-01-15"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDUU8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDUU8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUU8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUU8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDUU8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUU8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDUU8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger2 XE 9M","resolution":72.0},"name":"Advanced Photon Source (APS), Argonne National Laboratory","city":"Lemont, IL","country":"USA","beamline_name":"BioCAT 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Michigan","webpage":null}],"contributor_name":"Aldrex","contributor_surname":"Munsayac","orcid":"https://orcid.org/0000-0001-7268-020X"}],"concentration_method":null,"concentration_unit":null,"date":"2022-02-20","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":0.5,"number_of_frames":47,"wavelength":0.1033,"sample_detector_distance":3.678,"concentration_min":null,"concentration_max":3.4,"sample_volume":250.0,"flow_rate":0.6,"s_min":0.028,"s_max":4.157,"total_exposure_time":null,"seccolumn":3},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDUU8_fit1_model1_img.png","software":"RNAMasonry","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDUU8_fit1_model1.pdb","model_mw":29.03,"bead_radius":1.9,"log":null,"symmetry":null,"comment":"Generated using AlphaFold3 and RNAMasonry software(s)","user":788}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDUU8_fit1_fitfoxs_img.png","software":"FoXS","chi_square_value":1.105697,"p_value":0.0063,"fit_residual_plot":"SASDUU8_fit1_fitfoxsresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDUU8_fit1.dat","fit_log":null,"software_version":null,"description":""},{"models":[{"model_plot":null,"software":"DENSS","pdb_link":[],"model_title":null,"type_of_model":"density","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDUU8_fit2_model1.mrc","model_mw":null,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":788}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDUU8_fit2_fixed_fit_img.png","software":"DENSS","chi_square_value":2.9866,"p_value":1.0,"fit_residual_plot":"SASDUU8_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDUU8_fit2.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the HIV-1 DIS-C:DIS-Gk RNA heterocomplex in 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, pH 7.5 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Eiger2 XE 9M (Dectris) detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 3.4 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. 47 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":30.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.03392,"pddf_i0_error":4e-05,"guinier_i0":0.0337711,"guinier_i0_error":4e-05,"pddf_rg":3.651,"pddf_rg_error":0.1,"guinier_rg":3.531,"guinier_rg_error":0.11,"pddf_dmax":13.5,"pddf_dmax_error":null,"porod_volume":40.3,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":207,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDUU8_datloglog_img.png","symmetry":null,"last_modified":"2025-02-12T14:50:26.570275+01:00","bragg_peak":[]}