{"code":"SASDUV6","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Phase separation of a microtubule plus-end tracking protein into a fluid fractal network","publication":{"title":"Phase separation of a microtubule plus-end tracking protein into a fluid fractal network","author_list":"Czub M, Uliana F, Grubić T, Padeste C, Rosowski K, Dufresne E, Menzel A, Vakonakis I, Gasser U, Steinmetz M","journal":null,"doi":"10.1101/2024.04.19.590270","pmid":null,"published_date":"2024 Apr 19"},"status":"released","submitted_date":"2024-04-06","released_date":"2024-07-15"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDUV6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDUV6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUV6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUV6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDUV6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUV6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDUV6.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger 4M","resolution":75.0},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Nuclear fusion protein BIK1","short_name":"Bik1 ΔQQFF","sequence":"MAHHHHHHSAALEVLFQGPDRYQRKIGCFIQIPNLGRGQLKYVGPVDTKAGMFAGVDLLANIGKNDGSFMGKKYFQTEYPQSGLFIQLQKVASLIEKASISQTSRRTTMEPLSIPKNRSIVRLTNQFSPMDDPKSPTPMRSFRITSRHSGNQQSMDQEASDHHQQQEFGYDNREDRMEVDSILSSDRKANHNTTSDWKPDNGHMNDLNSSEVTIELREAQLTIEKLQRKQLHYKRLLDDQRMVLEEVQPTFDRYEATIQEREKEIDHLKQQLELERRQQAKQKQFFDAENEQLLAVVSQLHEEIKENEERNLSHNQPTGANEDVELLKKQLEQLRNIEDQFELHKTKWAKEREQLKMHNDSLSKEYQNLSKELFLTKPQDSSSEEVASLTKKLEEANEKIKQLEQAQAQTAVESLPIFDPPAPVDTTAGRQQWCEHCDTMGHNTAECPHHNPDN","organism":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","uniprot_code":"P11709","uniprot_range_first":2,"uniprot_range_last":436,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MDRYQRKIGCFIQIPNLGRGQLKYVGPVDTKAGMFAGVDLLANIGKNDGSFMGKKYFQTE\nYPQSGLFIQLQKVASLIEKASISQTSRRTTMEPLSIPKNRSIVRLTNQFSPMDDPKSPTP\nMRSFRITSRHSGNQQSMDQEASDHHQQQEFGYDNREDRMEVDSILSSDRKANHNTTSDWK\nPDNGHMNDLNSSEVTIELREAQLTIEKLQRKQLHYKRLLDDQRMVLEEVQPTFDRYEATI\nQEREKEIDHLKQQLELERRQQAKQKQFFDAENEQLLAVVSQLHEEIKENEERNLSHNQPT\nGANEDVELLKKQLEQLRNIEDQFELHKTKWAKEREQLKMHNDSLSKEYQNLSKELFLTKP\nQDSSSEEVASLTKKLEEANEKIKQLEQAQAQTAVESLPIFDPPAPVDTTAGRQQWCEHCD\nTMGHNTAECPHHNPDNQQFF","mw":52.548,"total_mw":105.096,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"The protein construct used for SAXS contains an additional polyhistidine tag and non-native amino acid extension at the N-terminus (MAHHHHHHSAALEVLFQGP)."}],"buffer":{"name":"20 mM Tris-HCl pH 7.5, 500 mM NaCl, 2% glycerol, 1 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":null,"deuteration":null},"purity_method":null,"name":"Nuclear fusion protein BIK1 ΔQQFF in a high salt buffer","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Paul Scherrer Institut","webpage":null}],"contributor_name":"Mateusz","contributor_surname":"Czub","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2023-07-13","storage_temperature":15.0,"cell_temperature":15.0,"exposure_time":3.2,"number_of_frames":41,"wavelength":0.09464,"sample_detector_distance":3.7197,"concentration_min":null,"concentration_max":6.0,"sample_volume":50.0,"flow_rate":0.16,"s_min":0.045,"s_max":3.4,"total_exposure_time":null,"seccolumn":43},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDUV6_fit1_model1_img.png","software":"DAMFILT","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"DAMFILT 5.0 (r12736)","model_data":"https://www.sasbdb.org/media/pdb_file/SASDUV6_fit1_model1.pdb","model_mw":253.51,"bead_radius":5.25,"log":null,"symmetry":"P1","comment":null,"user":1053}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDUV6_fit1_fixed_fit_img.png","software":"DAMMIF","chi_square_value":1.029,"p_value":0.1527,"fit_residual_plot":"SASDUV6_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDUV6_fit1.fir","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"Note: The ab initio model displayed in this entry represents the bead-occupancy and volume-corrected shape obtained from the spatial alignment of several individual DAM models (DAMFILT) and does not reflect the fit to the displayed data. CAUTION: Dmax severely underestimated.","experiment_description":"Synchrotron SAXS data from solutions of BIK1 ΔQQFF in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 2% glycerol, 1 mM DTT were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.09464 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 6 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW404-4F column at 15°C. 41 successive 3.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":124.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.06721,"pddf_i0_error":null,"guinier_i0":0.0682791,"guinier_i0_error":null,"pddf_rg":9.48,"pddf_rg_error":null,"guinier_rg":9.4,"guinier_rg_error":3.298,"pddf_dmax":29.4,"pddf_dmax_error":null,"porod_volume":632.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":16,"guinier_point_last":39,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDUV6_datloglog_img.png","symmetry":null,"last_modified":"2024-07-15T07:49:37.845278+02:00","bragg_peak":[]}