{"code":"SASDUX6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2.","publication":{"title":"Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2.","author_list":"Jamwal A, Colomb F, McSorley HJ, Higgins MK","journal":"Nat Commun","doi":"10.1038/s41467-024-49550-0","pmid":"38890291","published_date":"2024 Jun 19"},"status":"released","submitted_date":"2024-04-10","released_date":"2024-04-25"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDUX6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDUX6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUX6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUX6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDUX6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUX6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDUX6.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger 4M","resolution":75.0},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Alarmin release inhibitor (Δ1-125; N175Q, N190Q)","short_name":"HpARI2_CC23","sequence":"GCKGILEMLFDMPKEERPSPMYDSVTYDPTPNTPTTVGKDGIWNGVDYRQGSTVKPYCDTGPVIQGSSKAVCVSGKWVPTLGVCPKMCSIGSLKENGKFVDVTATTKGDELNPPPREQTLIPIVRKVDKDKVQHGVKVVALCKAEDSTTAAEGVQEFECDNGKWKPEPVPCP","organism":"Heligmosomoides polygyrus","uniprot_code":"A0A3P7XL18","uniprot_range_first":126,"uniprot_range_last":297,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MDIIGQVYCFTGAPHTITCLLREQFINWYIKYKRSENYPAFRELVVMYRLFLALGFLTFI\nNAAGQRCRFTDVERDKGYTGMLLKGRLRKTAGNGRTVELICGRGNHNYTCESGVLKESSP\nRQARCGCKGILEMLFDMPKEERPSPMYDSVTYDPTPNTPTTVGKDGIWNGVDYRNGSTVK\nPYCDTGPVINGSSKAVCVSGKWVPTLGVCPKMCSIGSLKENGKFVDVTATTKGDELNPPP\nREQTLIPIVRKVDKDKVQHGVKVVALCKAEDSTTAAEGVQEFECDNGKWKPEPVPCP","mw":18.615,"total_mw":18.615,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.2,"deuteration":null},"purity_method":null,"name":"Alarmin release inhibitor (HpARI2) domains CCP2 and CCP3","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Department of Biochemistry, University of Oxford","webpage":null}],"contributor_name":"Matt","contributor_surname":"Higgins","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2024-02-09","storage_temperature":15.0,"cell_temperature":15.0,"exposure_time":3.0,"number_of_frames":600,"wavelength":0.09464,"sample_detector_distance":3.7025,"concentration_min":null,"concentration_max":7.0,"sample_volume":null,"flow_rate":null,"s_min":0.045,"s_max":3.4,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDUX6_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDUX6_fit1_model1.pdb","model_mw":null,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":1054}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDUX6_fit1_fixed_fit_img.png","software":"DAMMIF","chi_square_value":0.982,"p_value":0.0024,"fit_residual_plot":"SASDUX6_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDUX6_fit1.fir","fit_log":null,"software_version":null,"description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDUX6_fit2_model1_img.png","software":"Chimera","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDUX6_fit2_model1.pdb","model_mw":17.36,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":1054}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDUX6_fit2_fixed_fit_img.png","software":"CRYSOL","chi_square_value":2.402,"p_value":0.0,"fit_residual_plot":"SASDUX6_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDUX6_fit2.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the alarmin release inhibitor domains CCP2 and CCP3 in 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH 7.2 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.09464 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.00 mg/ml was measured at 15°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":19.04,"experimental_mw_error":null,"guinier_i0_mw":16.52,"guinier_i0_mw_error":null,"porod_mw":17.0,"porod_mw_error":null,"pddf_i0":0.01695,"pddf_i0_error":null,"guinier_i0":0.0168623,"guinier_i0_error":null,"pddf_rg":2.34,"pddf_rg_error":null,"guinier_rg":2.266,"guinier_rg_error":0.077,"pddf_dmax":8.19,"pddf_dmax_error":null,"porod_volume":27.8,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":58,"guinier_point_last":404,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDUX6_datloglog_img.png","symmetry":null,"last_modified":"2024-06-23T14:27:49.757808+02:00","bragg_peak":[]}