{"code":"SASDUY2","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"SAXS data of Mycobacterium tuberculosis SepF-C-terminal domain","publication":null,"status":"released","submitted_date":"2024-02-13","released_date":"2026-02-13"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDUY2.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDUY2.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUY2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUY2_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDUY2_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUY2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDUY2.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 2M","resolution":0.172},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"point","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Cell division protein SepF","short_name":"SepF","sequence":"MGHHHHHHENFYQGDGHPLSKITTLRPKDYSEARTIGERFRDGSPVIMDLVSMDNADAKRLVDFAAGLAFALRGSFDKVATKVFLLSPADVDVSPEERRRIAETGFYAYQ","organism":"Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)","uniprot_code":"P9WGJ5","uniprot_range_first":123,"uniprot_range_last":218,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSTLHKVKAYFGMAPMEDYDDEYYDDRAPSRGYARPRFDDDYGRYDGRDYDDARSDSRGD\nLRGEPADYPPPGYRGGYADEPRFRPREFDRAEMTRPRFGSWLRNSTRGALAMDPRRMAMM\nFEDGHPLSKITTLRPKDYSEARTIGERFRDGSPVIMDLVSMDNADAKRLVDFAAGLAFAL\nRGSFDKVATKVFLLSPADVDVSPEERRRIAETGFYAYQ","mw":12.402,"total_mw":24.804,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"C-terminal domain of cell division protein SepF. The protein construct used for SAXS contains a N-terminal polyhistidine tag (MGHHHHHHENFYQG)."}],"buffer":{"name":"Tris 25 mM, 100 mM NaCl, 10% glycerol, 1 mM EDTA","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.5,"deuteration":null},"purity_method":null,"name":"Polyhistidine tagged Cell division protein SepF C-terminal domain (2 mg/mL) from Mycobacterium tuberculosis","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"CSIR","address":"Chandigarh, India","full_name":"CSIR-Institute of Microbial Technology","webpage":"http://www.imtech.res.in/"}],"contributor_name":"Jagrity","contributor_surname":"Choudhury","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2018-11-25","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.5,"number_of_frames":10,"wavelength":0.1,"sample_detector_distance":2.9,"concentration_min":null,"concentration_max":2.0,"sample_volume":null,"flow_rate":null,"s_min":0.038,"s_max":4.946,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of cell division protein SepF (C-terminal domain) in 25 mM Tris, 100 mM NaCl, 10% glycerol, 1 mM EDTA, pH 8.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":23.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":26.0,"porod_mw_error":null,"pddf_i0":21.95,"pddf_i0_error":null,"guinier_i0":21.7531,"guinier_i0_error":null,"pddf_rg":2.5,"pddf_rg_error":null,"guinier_rg":2.412,"guinier_rg_error":0.022,"pddf_dmax":8.8,"pddf_dmax_error":null,"porod_volume":41.7,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":18,"guinier_point_last":106,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDUY2_datloglog_img.png","symmetry":null,"last_modified":"2026-02-22T17:36:24.118611+01:00","bragg_peak":[]}