{"code":"SASDUY3","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes.","publication":{"title":"Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes.","author_list":"Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish","journal":"Int J Biol Macromol","doi":"10.1016/j.ijbiomac.2024.134556","pmid":"39128762","published_date":"2024 Aug 9"},"status":"released","submitted_date":"2024-02-28","released_date":"2024-03-08"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDUY3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUY3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUY3_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUY3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDUY3.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Gelsolin","short_name":"rhuGSN","sequence":"VRAATASRGASQAGAPQGRVPEARPNSMVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPTNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREVQGFESATFLGYFKSGLKYKKGGVASGFKHVVPNEVVVQRLFQVKGRRVVRATEVPVSWESFNNGDCFILDLGNNIHQWCGSNSNRYERLKATQVSKGIRDNERSGRARVHVSEEGTEPEAMLQVLGPKPALPAGTEDTAKEDAANRKLAKL\r\nYKVSNGAGTMSVSLVADENPFAQGALKSEDCFILDHGKDGKIFVWKGKQANTEERKAALKTASDFITKMDYPKQTQVSVLPEGGETPLFKQFFKNWRDPDQTDGLGLSYLSSHIANVERVPFDAATLHTSTAMAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGGTSREGGQTAPASTRLFQVRANSAGATRAVEVLPKAGALNSNDAFVLKTPSAAYLWVGTGASEAEKTGAQELLRVLRAQPVQVAEGSEPDGFWEALGGKAAYRTSPRLKDKKMDAHPPRLFACSNKIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLDRAMAELAATGHHHHHH","organism":"Homo sapiens","uniprot_code":"P06396","uniprot_range_first":25,"uniprot_range_last":782,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MAPHRPAPALLCALSLALCALSLPVRAATASRGASQAGAPQGRVPEARPNSMVVEHPEFL\nKAGKEPGLQIWRVEKFDLVPVPTNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNEC\nSQDESGAAAIFTVQLDDYLNGRAVQHREVQGFESATFLGYFKSGLKYKKGGVASGFKHVV\nPNEVVVQRLFQVKGRRVVRATEVPVSWESFNNGDCFILDLGNNIHQWCGSNSNRYERLKA\nTQVSKGIRDNERSGRARVHVSEEGTEPEAMLQVLGPKPALPAGTEDTAKEDAANRKLAKL\nYKVSNGAGTMSVSLVADENPFAQGALKSEDCFILDHGKDGKIFVWKGKQANTEERKAALK\nTASDFITKMDYPKQTQVSVLPEGGETPLFKQFFKNWRDPDQTDGLGLSYLSSHIANVERV\nPFDAATLHTSTAMAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNY\nRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFG\nGKPMIIYKGGTSREGGQTAPASTRLFQVRANSAGATRAVEVLPKAGALNSNDAFVLKTPS\nAAYLWVGTGASEAEKTGAQELLRVLRAQPVQVAEGSEPDGFWEALGGKAAYRTSPRLKDK\nKMDAHPPRLFACSNKIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEK\nTEALTSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLDRAMAEL\nAA","mw":84.265,"total_mw":84.265,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"The cloning, expression and purification of recombinant human gelsolin was performed as described previously [References: M.D. Badmalia, S. Singh, R. Garg, Ashish, Visualizing Temperature Mediated Activation of Gelsolin and Its Deactivation By Pip(2): A Saxs Based Study, Scientific reports 7(1) (2017) 4670, and  R. Garg, N. Peddada, A. Sagar, D. Nihalani, Ashish, Visual Insight into How Low pH Alone Can Induce Actin-severing Ability in Gelsolin under Calcium-free Conditions, J Biol Chem 286(23) (2011) 20387-20397.]"},{"long_name":"Actin, cytoplasmic 1","short_name":null,"sequence":"MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS\r\nKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT\r\nQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDL\r\nAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSY\r\nELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLS\r\nGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQ\r\nEYDESGPSIVHRKCF","organism":"Gallus gallus","uniprot_code":"P60706","uniprot_range_first":1,"uniprot_range_last":375,"oligomerization":"18-mer","molecular_type":"protein","uniprot_sequence":"MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS\nKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT\nQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDL\nAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSY\nELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLS\nGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQ\nEYDESGPSIVHRKCF","mw":41.736,"total_mw":751.248,"number_molecules":18,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol","concentration_unit":null,"comment":"F-buffer","additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Calcium activated full-length gelsolin and the F-form of actin at a 1:32 molar ratio in F-actin buffer","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"CSIR","address":"Chandigarh, India","full_name":"CSIR-Institute of Microbial Technology","webpage":"http://www.imtech.res.in/"}],"contributor_name":"FNU","contributor_surname":"Ashish","orcid":"https://orcid.org/0000-0002-0144-0441"}],"concentration_method":null,"concentration_unit":null,"date":"2013-08-31","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.155,"sample_detector_distance":3.1,"concentration_min":null,"concentration_max":2.0,"sample_volume":null,"flow_rate":null,"s_min":0.068,"s_max":4.435,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":665.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":47921.6,"guinier_i0_error":null,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":12.24,"guinier_rg_error":0.584,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":5,"guinier_point_last":15,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDUY3_datloglog_img.png","symmetry":null,"last_modified":"2024-08-12T08:58:33.945993+02:00","bragg_peak":[]}