{"code":"SASDV47","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"NADH-bound AIF activates the mitochondrial CHCHD4/MIA40 chaperone by a substrate-mimicry mechanism.","publication":{"title":"NADH-bound AIF activates the mitochondrial CHCHD4/MIA40 chaperone by a substrate-mimicry mechanism.","author_list":"Brosey CA, Shen R, Tainer JA","journal":"EMBO J","doi":"10.1038/s44318-024-00360-6","pmid":"39806100","published_date":"2025 Jan 13"},"status":"released","submitted_date":"2024-09-07","released_date":"2025-01-15"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDV47.dat","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDV47.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV47_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV47_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDV47_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV47_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDV47.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 2M","resolution":172.0},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Mitochondrial intermembrane space import and assembly protein 40","short_name":"CHCHD4","sequence":"MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPLEVLFQ","organism":"Homo sapiens","uniprot_code":"Q8N4Q1","uniprot_range_first":1,"uniprot_range_last":45,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMA\nSGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKP\nAEQAEETAPIEATATKEEEGSS","mw":5.876,"total_mw":11.752,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"This molecule is a fusion of AIF(104-613)-W196A and the N-terminus of CHCHD4 (residues 1-45) connected by a peptide linker (SGSGPGSGS). The chimera retains residues LEVLFQ at the C-terminus from the fusion tag."},{"long_name":"Apoptosis-inducing factor 1, mitochondrial","short_name":"AIF","sequence":"MLTPEQKQKKAALSASEGEEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFKQANGKERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRDNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHEDSGSGPGSGS","organism":"Homo sapiens","uniprot_code":"O95831","uniprot_range_first":104,"uniprot_range_last":613,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MFRCGGLAAGALKQKLVPLVRTVCVRSPRQRNRLPGNLFQRWHVPLELQMTRQMASSGAS\nGGKIDNSVLVLIVGLSTVGAGAYAYKTMKEDEKRYNERISGLGLTPEQKQKKAALSASEG\nEEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELW\nFSDDPNVTKTLRFKQWNGKERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRD\nNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISREV\nKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLSNWTMEKVRREG\nVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGG\nFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQS\nMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASE\nITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHED\nLNEVAKLFNIHED","mw":56.331,"total_mw":112.662,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"This molecule is a fusion of AIF(104-613)-W196A and the N-terminus of CHCHD4 (residues 1-45) connected by a peptide linker (SGSGPGSGS). The chimera retains residues LEVLFQ at the C-terminus from the fusion tag."}],"buffer":{"name":"25 mM HEPES, 150 mM NaCl, 2 mM TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Peptide-linked fusion protein of Apoptosis-inducing factor 1 AIF(104-613) point mutant W196A and the N-terminal segment of Mitochondrial intermembrane space import and assembly protein 40 CHCHD4(1-45)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Texas, USA","full_name":"MD Anderson Cancer Center","webpage":"https://www.mdanderson.org/"}],"contributor_name":"Chris","contributor_surname":"Brosey","orcid":"https://orcid.org/0000-0002-0990-0262"}],"concentration_method":null,"concentration_unit":null,"date":"2018-11-28","storage_temperature":4.0,"cell_temperature":10.0,"exposure_time":0.5,"number_of_frames":30,"wavelength":1.27,"sample_detector_distance":2.0,"concentration_min":null,"concentration_max":4.9,"sample_volume":null,"flow_rate":null,"s_min":0.114,"s_max":3.932,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ScÅtter","pddf_software_version":"3.0","i0_calibration_standard":null,"description":"Experimental molecular weight was determined by the volume of correlation (Vc) method. The real-space PDDF I(0) should be recorded as 193, based on ScAtter P(r) calculations.","experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":110.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":125.0,"porod_mw_error":null,"pddf_i0":193.0,"pddf_i0_error":0.21,"guinier_i0":198.121,"guinier_i0_error":null,"pddf_rg":3.85,"pddf_rg_error":0.022,"guinier_rg":3.869,"guinier_rg_error":0.01,"pddf_dmax":12.9,"pddf_dmax_error":null,"porod_volume":288.83,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":9,"guinier_point_last":41,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDV47_datloglog_img.png","symmetry":null,"last_modified":"2025-01-15T14:59:59.774869+01:00","bragg_peak":[]}