{"code":"SASDV52","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Aspergillus niger beta-glucosidase (bgl1)","publication":null,"status":"released","submitted_date":"2024-04-24","released_date":"2024-04-30"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDV52.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDV52.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV52_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV52_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/PTBP2_0284-0287s-new_kkEgYT3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV52_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDV52.sascif","experiment":{"instrument":{"detector":{"type":"duel detector","name":"Pilatus3 X 1M , Pilatus3 X 900K","resolution":null},"name":"National Synchrotron Light Source II (NSLS-II)","city":"Upton, NY","country":"USA","beamline_name":"16-ID (LiX)","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"beta-glucosidase","short_name":"bgl1","sequence":"MRFTLIEAVALTAVSLASADELAYSPPYYPSPWANGQGDWAQAYQRAVDIVSQMTLDEKVNLTTGTGWELELCVGQTGGVPRLGVPGMCLQDSPLGVRDSDYNSAFPAGMNVAATWDKNLAYLRGKAMGQEFSDKGADIQLGPAAGPLGRSPDGGRNWEGFSPDPALSGVLFAETIKGIQDAGVVATAKHYIAYEQEHFRQAPEAQGFGFNISESGSANLDDKTMHELYLWPFADAIRAGAGAVMCSYNQINNSYGCQNSYTLNKLLKAELGFQGFVMSDWAAHHAGVSGALAGLDMSMPGDVDYDSGTSYWGTNLTISVLNGTVPQWRVDDMAVRIMAAYYKVGRDRLWTPPNFSSWTRDEYGYKYYYVSEGPYEKVNQYVNVQRNHSELIRRIGADSTVLLKNDGALPLTGKERLVALIGEDAGSNPYGANGCSDRGCDNGTLAMGWGSGTANFPYLVTPEQAISNEVLKHKNGVFTATDNWAIDQIEALAKTASVSLVFVNADSGEGYINVDGNLGDRRNLTLWRNGDNVIKAAASNCNNTIVVIHSVGPVLVNEWYDNPNVTAILWGGLPGQESGNSLADVLYGRVNPGAKSPFTWGKTREAYQDYLVTEPNNGNGAPQEDFVEGVFIDYRGFDKRNETPIYEFGYGLSYTTFNYSNLEVQVLSAPAYEPASGETEAAPTFGEVGNASDYLYPSGLQRITKFIYPWLNGTDLEASSGDASYGQDSSDYLPEGATDGSAQPILPAGGGPGGNPRLYDELIRVSVTIKNTGKVAGDEVPQLYVSLGGPNEPKIVLRQFERITLQPSEETKWSTTLTRRDLANWNVEKQDWEITSYPKMVFVGSSSRKLPLRASLPTVH","organism":"Aspergillus niger","uniprot_code":"Q9P8F4","uniprot_range_first":1,"uniprot_range_last":860,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MRFTLIEAVALTAVSLASADELAYSPPYYPSPWANGQGDWAQAYQRAVDIVSQMTLDEKV\nNLTTGTGWELELCVGQTGGVPRLGVPGMCLQDSPLGVRDSDYNSAFPAGMNVAATWDKNL\nAYLRGKAMGQEFSDKGADIQLGPAAGPLGRSPDGGRNWEGFSPDPALSGVLFAETIKGIQ\nDAGVVATAKHYIAYEQEHFRQAPEAQGFGFNISESGSANLDDKTMHELYLWPFADAIRAG\nAGAVMCSYNQINNSYGCQNSYTLNKLLKAELGFQGFVMSDWAAHHAGVSGALAGLDMSMP\nGDVDYDSGTSYWGTNLTISVLNGTVPQWRVDDMAVRIMAAYYKVGRDRLWTPPNFSSWTR\nDEYGYKYYYVSEGPYEKVNQYVNVQRNHSELIRRIGADSTVLLKNDGALPLTGKERLVAL\nIGEDAGSNPYGANGCSDRGCDNGTLAMGWGSGTANFPYLVTPEQAISNEVLKHKNGVFTA\nTDNWAIDQIEALAKTASVSLVFVNADSGEGYINVDGNLGDRRNLTLWRNGDNVIKAAASN\nCNNTIVVIHSVGPVLVNEWYDNPNVTAILWGGLPGQESGNSLADVLYGRVNPGAKSPFTW\nGKTREAYQDYLVTEPNNGNGAPQEDFVEGVFIDYRGFDKRNETPIYEFGYGLSYTTFNYS\nNLEVQVLSAPAYEPASGETEAAPTFGEVGNASDYLYPSGLQRITKFIYPWLNGTDLEASS\nGDASYGQDSSDYLPEGATDGSAQPILPAGGGPGGNPRLYDELIRVSVTIKNTGKVAGDEV\nPQLYVSLGGPNEPKIVLRQFERITLQPSEETKWSTTLTRRDLANWNVEKQDWEITSYPKM\nVFVGSSSRKLPLRASLPTVH","mw":93.345,"total_mw":186.69,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"Purified protein from native Aspergillus niger glucosidase crude product."}],"buffer":{"name":"50 mM sodium acetate","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":5.0,"deuteration":null},"purity_method":null,"name":"Aspergillus niger beta-glucosidase (bgl1), native, glycosylated","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Brookhaven National Laboratory","webpage":null}],"contributor_name":"Estella","contributor_surname":"Yee","orcid":"https://orcid.org/0000-0001-7108-1387"}],"concentration_method":null,"concentration_unit":null,"date":"2024-06-04","storage_temperature":4.0,"cell_temperature":null,"exposure_time":1.0,"number_of_frames":5,"wavelength":0.08189,"sample_detector_distance":4.003,"concentration_min":null,"concentration_max":9.5,"sample_volume":54.0,"flow_rate":0.35,"s_min":0.06,"s_max":31.9,"total_exposure_time":null,"seccolumn":2},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDV52_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDV52_fit1_model1.cif","model_mw":null,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/dammin-slow-8.log","symmetry":"P1","comment":"Dammin was run on the most representative model of 10 generated by GASBOR.","user":889}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDV52_fit1_fixed_fit_img.png","software":"DAMMIN","chi_square_value":0.719,"p_value":0.5363,"fit_residual_plot":"SASDV52_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDV52_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/dammin-slow-8_zAp32QL.log","software_version":null,"description":"Dammin was run on the most representative model of 10 generated by GASBOR."}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Aspergillus niger beta-glucosidase (bgl1), native, glycosylated in 50 mM sodium acetate, pH 5 were collected on the 16-ID (LiX) beamline at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 X 1M, Pilatus3 X 900K dual detectors at a sample-detector distance of 4.003 m (SAXS) and 0.282 m (WAXS) at a wavelength of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 54.00 μl sample at 9.5 mg/ml was injected at a 0.35 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column. Five successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":195.2,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":230.0,"porod_mw_error":null,"pddf_i0":24.93,"pddf_i0_error":-2e-10,"guinier_i0":24.8383,"guinier_i0_error":null,"pddf_rg":4.45,"pddf_rg_error":null,"guinier_rg":4.396,"guinier_rg_error":0.166,"pddf_dmax":13.9,"pddf_dmax_error":null,"porod_volume":277.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":3,"guinier_point_last":24,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDV52_datloglog_img.png","symmetry":null,"last_modified":"2024-07-02T12:09:46.992729+02:00","bragg_peak":[]}