{"code":"SASDV57","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation","publication":{"title":"Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation","author_list":"Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C","journal":"Acta Crystallographica Section D Structural Biology","doi":"10.1107/S2059798325005303","pmid":null,"published_date":"2025 Jun 27"},"status":"released","submitted_date":"2024-09-18","released_date":"2025-06-29"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDV57.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDV57.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV57_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV57_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDV57_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV57_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDV57.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 S 2M","resolution":172.0},"name":"Australian Synchrotron","city":"Melbourne","country":"Australia","beamline_name":"SAXS/WAXS","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Splicing factor, proline- and glutamine-rich","short_name":"SFPQ cysteine dimer","sequence":"GAMSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRMGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF","organism":"Homo sapiens","uniprot_code":"P23246","uniprot_range_first":214,"uniprot_range_last":707,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPI\nPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPG\nVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQ\nAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGE\nPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLF\nVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGR\nQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFAS\nKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRF\nAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLM\nRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRM\nGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGS\nDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF","mw":76.277,"total_mw":152.554,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"R542C mutation causes monomers of SFPQ to dimerise via the coiled-coil domain (Lee et al, 2015)"}],"buffer":{"name":"500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Full-length SFPQ in high salt buffer","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[],"concentration_method":null,"concentration_unit":null,"date":"2022-10-22","storage_temperature":25.0,"cell_temperature":25.0,"exposure_time":1.0,"number_of_frames":700,"wavelength":0.10781,"sample_detector_distance":2.79,"concentration_min":null,"concentration_max":4.2,"sample_volume":60.0,"flow_rate":0.4,"s_min":0.051,"s_max":5.704,"total_exposure_time":null,"seccolumn":2},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"A dimer of full-length SFPQ at 4.2mg/ml in 500mM KNO3, 20mM HEPES (pH 7.4), 5% glycerol, 1mM DTT. \r\n\r\nMeasurement was taken at the Australian Synchrotron SAXS/WAXS beamline and is part of the manuscript ' Structural dynamics of IDR interactions in human SFPQ and implications for liquid-liquid phase separation '.","experiment_description":"Synchrotron SAXS data from solutions of Dimeric full-length human Splicing factor, proline- and glutamine-rich (SFPQ) in 500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH 7.4 were collected on the SAXS/WAXS beam line at the Australian Synchrotron storage ring (Melbourne, Australia) using a Pilatus3 S 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.10781 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 4.2 mg/ml was injected at a 0.40 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 25°C. 450 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. A dimer of full-length SFPQ at 4.2mg/ml in 500mM KNO3, 20mM HEPES (pH 7.4), 5% glycerol, 1mM DTT. Measurement was taken at the Australian Synchrotron SAXS/WAXS beamline and is part of the manuscript ' Structural dynamics of IDR interactions in human SFPQ and implications for liquid-liquid phase separation '.","tags":[],"intensity_unit":"1/cm","experimental_mw":200.46,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":380.0,"porod_mw_error":null,"pddf_i0":0.02728,"pddf_i0_error":null,"guinier_i0":0.0259164,"guinier_i0_error":null,"pddf_rg":9.5,"pddf_rg_error":null,"guinier_rg":7.935,"guinier_rg_error":1.807,"pddf_dmax":42.5,"pddf_dmax_error":null,"porod_volume":485.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":3,"guinier_point_last":31,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDV57_datloglog_img.png","symmetry":null,"last_modified":"2025-07-03T11:05:26.761664+02:00","bragg_peak":[]}