{"code":"SASDV59","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation","publication":{"title":"Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation","author_list":"Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C","journal":"Acta Crystallographica Section D Structural Biology","doi":"10.1107/S2059798325005303","pmid":null,"published_date":"2025 Jun 27"},"status":"released","submitted_date":"2024-09-18","released_date":"2025-06-29"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDV59.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDV59.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV59_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV59_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDV59_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV59_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDV59.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"ORDELA/21000N","resolution":5.1},"name":"Australian Centre for Neutron Scattering (ANSTO)","city":"Lucas Heights, Sydney","country":"Australia","beamline_name":"Quokka - Small Angle Neutron Scattering","beam_geometry":null,"type_of_source":"neutron source","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Splicing factor, proline- and glutamine-rich","short_name":"SFPQ cysteine dimer","sequence":"GAMSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRMGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF","organism":"Homo sapiens","uniprot_code":"P23246","uniprot_range_first":214,"uniprot_range_last":707,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPI\nPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPG\nVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQ\nAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGE\nPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLF\nVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGR\nQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFAS\nKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRF\nAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLM\nRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRM\nGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGS\nDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF","mw":76.277,"total_mw":152.554,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"R542C mutation causes monomers of SFPQ to dimerise via the coiled-coil domain (Lee et al, 2015)"}],"buffer":{"name":"150 mM KCl, 0.075% glycerol,  20 mM HEPES, 0.5 mM DTT,","concentration_unit":null,"comment":"SANS contrast matching buffer","additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":95.0},"purity_method":null,"name":"Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95%","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"The University of Western Australia","webpage":null}],"contributor_name":"Heidar","contributor_surname":"Koning","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2023-01-20","storage_temperature":25.0,"cell_temperature":25.0,"exposure_time":null,"number_of_frames":null,"wavelength":0.6,"sample_detector_distance":1.3,"concentration_min":null,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.082,"s_max":4.352,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"SANS experiment indicating dimer partner exchange between deuterated and protiated monomers of full-length SFPQ. Contrast-matching has ensured signal is coming from protiated monomers of full-length SFPQ.\r\n\r\nAdditional details are available in the manuscript: Structural dynamics of IDR interactions in human SFPQ and implications for liquid-liquid phase separation.","experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":41.25,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":113.0,"porod_mw_error":null,"pddf_i0":0.02926,"pddf_i0_error":null,"guinier_i0":0.0285187,"guinier_i0_error":null,"pddf_rg":6.64,"pddf_rg_error":null,"guinier_rg":6.109,"guinier_rg_error":0.418,"pddf_dmax":22.0,"pddf_dmax_error":null,"porod_volume":181.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":7,"guinier_point_last":19,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDV59_datloglog_img.png","symmetry":null,"last_modified":"2025-07-03T11:05:27.816508+02:00","bragg_peak":[]}