{"code":"SASDV65","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structural dynamics of protein-protein association involved in the light-induced transition of Avena sativa LOV2 protein.","publication":{"title":"Structural dynamics of protein-protein association involved in the light-induced transition of Avena sativa LOV2 protein.","author_list":"Kim C, Yun SR, Lee SJ, Kim SO, Lee H, Choi J, Kim JG, Kim TW, You S, Kosheleva I, Noh T, Baek J, Ihee H","journal":"Nat Commun","doi":"10.1038/s41467-024-51461-z","pmid":"39143073","published_date":"2024 Aug 14"},"status":"released","submitted_date":"2024-07-11","released_date":"2024-07-27"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDV65.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV65_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV65_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV65_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDV65.sascif","experiment":{"instrument":{"detector":{"type":"CCD","name":"Rayonix SX165","resolution":15.0},"name":"Pohang Accelerator Laboratory","city":"Pohang","country":"South Korea","beamline_name":"4C","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"non-specific serine/threonine protein kinase","short_name":null,"sequence":"FLATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL","organism":"Avena sativa","uniprot_code":"O49003","uniprot_range_first":404,"uniprot_range_last":546,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MASKGAGGGGGGHEEPQRPKQQLPRDSRGSLEVFNPSSSSAAVEPPSAFRPAARSASPFI\nEEATGGIEDVGKATQRAAEWGLVLQTNEQTGRPQGVSARSSGGGGSARSSSDDKAVAGAI\nPRVSEELRAALSAFQQTFVVSDASRPGHPIMYASAGFFNMTGYTSKEVVGRNCRFLQGSG\nTDPAEIAKIRQALANGSNYCGRVLNYKKDGTAFWNLLTIAPIKDEEGRVLKFIGMQVEVS\nKYTEGNKDTVVRPNGLPESLIKYDARQKDQARSSVSELLLAIKNPRSLSESTNSTFKRKS\nQESVGALTGDRPGKRSSESGSRRNSKSGARTSLQKISEVPERGSKSRKSGLYSLMSLLGM\nGPGNIEKDMLKPRDEDPLLDSDDERPESFDDELRRKEMRRGIDLATTLERIEKNFVITDP\nRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQL\nINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENID\nEAAKELPDANLRPEDLWANHSKVVLPKPHMKDSASWRAIQKVLEGGENIDLKHFRPVKPL\nGSGDTGSVHLVELLNTGEYFAMKAMDKNVMLNRNKVHRANAEREILDMLDHPFLPTLYAS\nFQTKTHICLITDYYPGGELFLLLDRQPLKVLREDAVRFYAAEVVIALEYLHCQGIIYRDL\nKPENILLHRDGHISLTDFDLSCLTSCRPQVFLPEEANKKSRRKSRSSPIFFAEPMRASNS\nFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKDIRF\nPASISVSLPARQLIYRLLHRDPSNRLGSYEGSNEIKEHPFFRGINWALVRGTAPPKLDAP\nLFPDDTDKGMGDAAAADTHTDMF","mw":16.645,"total_mw":33.29,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"Tandem PAS-PAC domains."}],"buffer":{"name":"20 mM Tris, 200 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"The light-adapted AsLOV2 domain (Avena sativa, Oat)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Korea Advanced Institute of Science and Technology","webpage":null}],"contributor_name":"Hyotcherl","contributor_surname":"Ihee","orcid":"https://orcid.org/0000-0003-0397-5965"}],"concentration_method":null,"concentration_unit":null,"date":"2021-05-15","storage_temperature":24.0,"cell_temperature":24.0,"exposure_time":10.0,"number_of_frames":50,"wavelength":0.0734,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":24.98,"sample_volume":null,"flow_rate":null,"s_min":0.252,"s_max":5.999,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":"The SAXS data of AsLOV2 in the light-adapted state were measured where the target sample was continuously illuminated at a wavelength of 455 nm.","experiment_description":"Synchrotron SAXS data from solutions of light-adapted AsLOV2 domain in 20 mM Tris, 200 mM NaCl, pH 7 were collected on the 4C beam line at the Pohang Accelerator Laboratory (Pohang, South Korea) using a Rayonix SX165 detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.0734 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 24.98 mg/ml was measured at 24°C. 50 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":33.3,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":17.897,"guinier_i0_error":null,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":2.26,"guinier_rg_error":0.014,"pddf_dmax":8.5,"pddf_dmax_error":null,"porod_volume":51.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":210,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDV65_datloglog_img.png","symmetry":null,"last_modified":"2024-10-07T14:53:56.454816+02:00","bragg_peak":[]}