{"code":"SASDV67","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation","publication":{"title":"Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation","author_list":"Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C","journal":"Acta Crystallographica Section D Structural Biology","doi":"10.1107/S2059798325005303","pmid":null,"published_date":"2025 Jun 27"},"status":"released","submitted_date":"2024-09-18","released_date":"2025-06-29"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDV67.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDV67.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV67_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV67_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDV67_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV67_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDV67.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 S 2M","resolution":172.0},"name":"Australian Synchrotron","city":"Melbourne","country":"Australia","beamline_name":"SAXS/WAXS","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Splicing Factor Proline/Glutamine rich (1-598)","short_name":"SFPQ1-598","sequence":"GAMSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYS","organism":"Homo sapiens","uniprot_code":"P23246","uniprot_range_first":1,"uniprot_range_last":598,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPI\nPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPG\nVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQ\nAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGE\nPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLF\nVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGR\nQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFAS\nKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRF\nAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLM\nRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRM\nGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGS\nDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF","mw":65.148,"total_mw":130.296,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"The University of Western Australia","webpage":null}],"contributor_name":"Heidar","contributor_surname":"Koning","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2023-03-13","storage_temperature":25.0,"cell_temperature":25.0,"exposure_time":1.0,"number_of_frames":400,"wavelength":0.10781,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.047,"s_max":4.92,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"SFPQ as a dimer truncated to remove the C-terminal Intrinsically Disordered Region (IDR).\r\n\r\nExperiment carried out at the SAXS/WAXS beamline with protein at 5mg/ml in 500mM KNO3, 20mM HEPES (pH 7.4), 5% glycerol, 1mM DTT. \r\n\r\nThis data is part of the manuscript ' Structural dynamics of IDR interactions in human SFPQ and implications for liquid-liquid phase separation '.","experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":161.077,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":331.0,"porod_mw_error":null,"pddf_i0":0.03449,"pddf_i0_error":null,"guinier_i0":0.0335721,"guinier_i0_error":null,"pddf_rg":8.37,"pddf_rg_error":null,"guinier_rg":7.555,"guinier_rg_error":0.39,"pddf_dmax":34.3,"pddf_dmax_error":null,"porod_volume":400.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":2,"guinier_point_last":36,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDV67_datloglog_img.png","symmetry":null,"last_modified":"2025-07-03T11:05:27.067416+02:00","bragg_peak":[]}