{"code":"SASDV88","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"The UvrA2B2  pre-initiation complex from  the Nucleotide Excision Repair pathway of Mycobacterium tuberculosis.","publication":null,"status":"released","submitted_date":"2024-10-15","released_date":"2025-10-15"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDV88.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDV88.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV88_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV88_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDV88_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV88_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDV88.sascif","experiment":{"instrument":{"detector":{"type":"Hybrid Photon Counting (HPC)","name":"Mythen2 R 1K","resolution":null},"name":"CSIR-Central Drug Research Institute","city":"Lucknow","country":"India","beamline_name":"Anton Paar SAXSpace","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"UvrABC system protein A","short_name":"UvrA","sequence":"MADRLIVKGAREHNLRSVDLDLPRDALIVFTGLSGSGKSSLAFDTIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNRNPRSTVGTITEVYDYLRLLYARAGTPHCPTCGERVARQTPQQIVDQVLAMPEGTRFLVLAPVVRTRKGEFADLFDKLNAQGYSRVRVDGVVHPLTDPPKLKKQEKHDIEVVVDRLTVKAAAKRRLTDSVETALNLADGIVVLEFVDHELGAPHREQRFSEKLACPNGHALAVDDLEPRSFSFNSPYGACPECSGLGIRKEVDPELVVPDPDRTLAQGAVAPWSNGHTAEYFTRMMAGLGEALGFDVDTPWRKLPAKARKAILEGADEQVHVRYRNRYGRTRSYYADFEGVLAFLQRKMSQTESEQMKERYEGFMRDVPCPVCAGTRLKPEILAVTLAGESKGEHGAKSIAEVCELSIADCADFLNALTLGPREQAIAGQVLKEIRSRLGFLLDVGLEYLSLSRAAATLSGGEAQRIRLATQIGSGLVGVLYVLDEPSIGLHQRDNRRLIETLTRLRDLGNTLIVVEHDEDTIEHADWIVDIGPGAGEHGGRIVHSGPYDELLRNKDSITGAYLSGRESIEIPAIRRSVDPRRQLTVVGAREHNLRGIDVSFPLGVLTSVTGVSGSGKSTLVNDILAAVLANRLNGARQVPGRHTRVTGLDYLDKLVRVDQSPIGRTPRSNPATYTGVFDKIRTLFAATTEAKVRGYQPGRFSFNVKGGRCEACTGDGTIKIEMNFLPDVYVPCEVCQGARYNRETLEVHYKGKTVSEVLDMSIEEAAEFFEPIAGVHRYLRTLVDVGLGYVRLGQPAPTLSGGEAQRVKLASELQKRSTGRTVYILDEPTTGLHFDDIRKLLNVINGLVDKGNTVIVIEHNLDVIKTSDWIIDLGPEGGAGGGTVVAQGTPEDVAAVPASYTGKFLAEVVGGGASAATSRSNRRRNVSA","organism":"Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)","uniprot_code":"P9WQK7","uniprot_range_first":1,"uniprot_range_last":972,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MADRLIVKGAREHNLRSVDLDLPRDALIVFTGLSGSGKSSLAFDTIFAEGQRRYVESLSA\nYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNRNPRSTVGTITEVYDYLRLLYARAGTPH\nCPTCGERVARQTPQQIVDQVLAMPEGTRFLVLAPVVRTRKGEFADLFDKLNAQGYSRVRV\nDGVVHPLTDPPKLKKQEKHDIEVVVDRLTVKAAAKRRLTDSVETALNLADGIVVLEFVDH\nELGAPHREQRFSEKLACPNGHALAVDDLEPRSFSFNSPYGACPECSGLGIRKEVDPELVV\nPDPDRTLAQGAVAPWSNGHTAEYFTRMMAGLGEALGFDVDTPWRKLPAKARKAILEGADE\nQVHVRYRNRYGRTRSYYADFEGVLAFLQRKMSQTESEQMKERYEGFMRDVPCPVCAGTRL\nKPEILAVTLAGESKGEHGAKSIAEVCELSIADCADFLNALTLGPREQAIAGQVLKEIRSR\nLGFLLDVGLEYLSLSRAAATLSGGEAQRIRLATQIGSGLVGVLYVLDEPSIGLHQRDNRR\nLIETLTRLRDLGNTLIVVEHDEDTIEHADWIVDIGPGAGEHGGRIVHSGPYDELLRNKDS\nITGAYLSGRESIEIPAIRRSVDPRRQLTVVGAREHNLRGIDVSFPLGVLTSVTGVSGSGK\nSTLVNDILAAVLANRLNGARQVPGRHTRVTGLDYLDKLVRVDQSPIGRTPRSNPATYTGV\nFDKIRTLFAATTEAKVRGYQPGRFSFNVKGGRCEACTGDGTIKIEMNFLPDVYVPCEVCQ\nGARYNRETLEVHYKGKTVSEVLDMSIEEAAEFFEPIAGVHRYLRTLVDVGLGYVRLGQPA\nPTLSGGEAQRVKLASELQKRSTGRTVYILDEPTTGLHFDDIRKLLNVINGLVDKGNTVIV\nIEHNLDVIKTSDWIIDLGPEGGAGGGTVVAQGTPEDVAAVPASYTGKFLAEVVGGGASAA\nTSRSNRRRNVSA","mw":106.13,"total_mw":212.26,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""},{"long_name":"UvrABC system protein B","short_name":"UvrB","sequence":"MAFATEHPVVAHSEYRAVEEIVRAGGHFEVVSPHAPAGDQPAAIDELERRINAGERDVVLLGATGTGKSATTAWLIERLQRPTLVMAPNKTLAAQLANELREMLPHNAVEYFVSYYDYYQPEAYIAQTDTYIEKDSSINDDVERLRHSATSALLSRRDVVVVASVSCIYGLGTPQSYLDRSVELKVGEEVPRDGLLRLLVDVQYTRNDMSFTRGSFRVRGDTVEIIPSYEELAVRIEFFGDEIEALYYLHPLTGEVIRQVDSLRIFPATHYVAGPERMAHAVSAIEEELAERLAELESQGKLLEAQRLRMRTNYDIEMMRQVGFCSGIENYSRHIDGRGPGTPPATLLDYFPEDFLLVIDESHVTVPQIGGMYEGDISRKRNLVEYGFRLPSACDNRPLTWEEFADRIGQTVYLSATPGPYELSQTGGEFVEQVIRPTGLVDPKVVVKPTKGQIDDLIGEIRTRADADQRVLVTTLTKKMAEDLTDYLLEMGIRVRYLHSEVDTLRRVELLRQLRLGDYDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSSRSLIQTIGRAARNVSGEVHMYADKITDSMREAIDETERRRAKQIAYNEANGIDPQPLRKKIADILDQVYREADDTAVVEVGGSGRNASRGRRAQGEPGRAVSAGVFEGRDTSAMPRAELADLIKDLTAQMMAAARDLQFELAARFRDEIADLKRELRGMDAAGLK","organism":"Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)","uniprot_code":"P9WFC7","uniprot_range_first":1,"uniprot_range_last":719,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MAFATEHPVVAHSEYRAVEEIVRAGGHFEVVSPHAPAGDQPAAIDELERRINAGERDVVL\nLGATGTGKSATTAWLIERLQRPTLVMAPNKTLAAQLANELREMLPHNAVEYFVSYYDYYQ\nPEAYIAQTDTYIEKDSSINDDVERLRHSATSALLSRRDVVVVASVSCIYGLGTPQSYLDR\nSVELKVGEEVPRDGLLRLLVDVQYTRNDMSFTRGSFRVRGDTVEIIPSYEELAVRIEFFG\nDEIEALYYLHPLTGEVIRQVDSLRIFPATHYVAGPERMAHAVSAIEEELAERLAELESQG\nKLLEAQRLRMRTNYDIEMMRQVGFCSGIENYSRHIDGRGPGTPPATLLDYFPEDFLLVID\nESHVTVPQIGGMYEGDISRKRNLVEYGFRLPSACDNRPLTWEEFADRIGQTVYLSATPGP\nYELSQTGGEFVEQVIRPTGLVDPKVVVKPTKGQIDDLIGEIRTRADADQRVLVTTLTKKM\nAEDLTDYLLEMGIRVRYLHSEVDTLRRVELLRQLRLGDYDVLVGINLLREGLDLPEVSLV\nAILDADKEGFLRSSRSLIQTIGRAARNVSGEVHMYADKITDSMREAIDETERRRAKQIAY\nNEANGIDPQPLRKKIADILDQVYREADDTAVVEVGGSGRNASRGRRAQGEPGRAVSAGVF\nEGRDTSAMPRAELADLIKDLTAQMMAAARDLQFELAARFRDEIADLKRELRGMDAAGLK","mw":80.406,"total_mw":160.812,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"50 mM Tris pH 8, 200 mM NaCl, 2% glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":null,"deuteration":null},"purity_method":null,"name":"Mycobacterium tuberculosis UvrA2.UvrB2 complex","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"India","full_name":"CSIR - Central Drug Research Institute","webpage":"https://cdri.res.in/"}],"contributor_name":"farheen","contributor_surname":"jahan","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2022-06-06","storage_temperature":4.0,"cell_temperature":10.0,"exposure_time":30.0,"number_of_frames":2,"wavelength":0.154,"sample_detector_distance":320.0,"concentration_min":null,"concentration_max":0.9,"sample_volume":null,"flow_rate":null,"s_min":0.095,"s_max":7.584,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDV88_fit1_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDV88_fit1_model1.cif","model_mw":null,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/UvrA2B2_1.log","symmetry":null,"comment":null,"user":883}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDV88_fit1_fixed_fit_img.png","software":"GASBOR","chi_square_value":0.55,"p_value":0.0,"fit_residual_plot":"SASDV88_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDV88_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/UvrA2B2_1.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"Note: Data are of very-low quality. The experimental molecular weight cannot be obtained/confirmed from the SAXS data data. Due to the low concentration, the data contains noise in the sample—the complex of Mtb. UvrA dimer and UvrB dimer were purified by co-expressing His-tagged Mtb. UvrA and untagged Mtb.UvrB protein in BL-21 cells. The untagged UvrB forms a complex with UvrA (pre-initiation complex) and is purified. Further purification was performed by size exclusion chromatography using a superose 6 10/300 GL column, which shows the protein's equimolar and homogenous solution—the calculated molecular weight of the Mtb. UvrA2B2 complex using SEC is 343kDa.","experiment_description":"SAXS data from solutions of Mycobacterium tuberculosis UvrA2.UvrB2 complex in 50 mM Tris pH 8, 200 mM NaCl, 2% glycerol were collected using an Anton Paar SAXSpace instrument at the CSIR-Central Drug Research Institute (Lucknow India) equipped with a  Mythen2 R 1K detector at a sample-detector distance of 0.320 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.90 mg/ml was measured at 10°C. Two successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":343.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":658.0,"porod_mw_error":null,"pddf_i0":69930.0,"pddf_i0_error":null,"guinier_i0":63683.7,"guinier_i0_error":null,"pddf_rg":5.666,"pddf_rg_error":null,"guinier_rg":5.282,"guinier_rg_error":0.563,"pddf_dmax":15.84,"pddf_dmax_error":null,"porod_volume":1053.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":4,"guinier_point_last":27,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDV88_datloglog_img.png","symmetry":null,"last_modified":"2025-10-21T13:34:29.483230+02:00","bragg_peak":[]}