{"code":"SASDVA7","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Probing the statistics of sequence-dependent DNA conformations in solution using SAXS.","publication":{"title":"Probing the statistics of sequence-dependent DNA conformations in solution using SAXS.","author_list":"Koning HJ, Pullakhandam A, Whitten AE, Bond CS, Peyrard M","journal":"Acta Crystallogr D Struct Biol","doi":"10.1107/S2059798325011556","pmid":"41553140","published_date":"2026 Feb 1"},"status":"released","submitted_date":"2024-09-18","released_date":"2025-08-12"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDVA7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVA7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVA7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVA7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVA7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVA7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVA7.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 S 2M","resolution":172.0},"name":"Australian Synchrotron","city":"Melbourne","country":"Australia","beamline_name":"SAXS/WAXS","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"GAGE6_3","short_name":"GAGE6_3","sequence":"GCCTTCTGCAAAGAAGTCTTGCGCATCGCGCGTGAAGGCGCGCGCTAGCGCGCGGATGCT","organism":"Homo sapiens","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"DNA","uniprot_sequence":"","mw":18.615,"total_mw":37.23,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"GAGE6_3 duplex \r\n\r\n5-GCCTTCTGCAAAGAAGTCTTGCGCATCGCGCGTGAAGGCGCGCGCTAGCGCGCGGATGCT-3"}],"buffer":{"name":"150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"The 60bp dsDNA oligo GAGE6_3","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"The University of Western Australia","webpage":null}],"contributor_name":"Heidar","contributor_surname":"Koning","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2023-11-28","storage_temperature":25.0,"cell_temperature":25.0,"exposure_time":1.0,"number_of_frames":400,"wavelength":0.10781,"sample_detector_distance":2.39,"concentration_min":null,"concentration_max":3.01,"sample_volume":null,"flow_rate":null,"s_min":0.068,"s_max":6.235,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of The 60bp dsDNA oligo GAGE6_2 in 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH 7.5 were collected on the SAXS/WAXS beam line at the Australian Synchrotron storage ring (Melbourne, Australia) using a Pilatus3 S 2M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.10781 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One 50 µl solute concentration of 2.78 mg/ml was measured at 25°C. 400 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. This data is part of the manuscript 'Probing the statistics of sequence-dependent DNA conformations in solution using SAXS'. 2sigma errors from the Australian Synchrotron's SAXS/WAXS beamline have been divided by 2 to be compatible with expectations from the ATSAS package.\r\n\r\n5-GCCTTCTGCAAAGAAGTCTTGCGCATCGCGCGTGAAGGCGCGCGCTAGCGCGCGGATGCT-3\r\n: The 60bp dsDNA duplex GAGE6_3","tags":[],"intensity_unit":"1/cm","experimental_mw":34.495,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":150.0,"porod_mw_error":null,"pddf_i0":0.01555,"pddf_i0_error":null,"guinier_i0":0.0150943,"guinier_i0_error":null,"pddf_rg":5.59,"pddf_rg_error":null,"guinier_rg":5.025,"guinier_rg_error":0.884,"pddf_dmax":21.4,"pddf_dmax_error":null,"porod_volume":80.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":22,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVA7_datloglog_img.png","symmetry":null,"last_modified":"2026-02-23T09:45:38.531837+01:00","bragg_peak":[]}