{"code":"SASDVB5","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"The oncogenic CCDC6-RET fusion protein is a dual ATP- and ADP-dependent kinase","publication":{"title":"The oncogenic CCDC6-RET fusion protein is a dual ATP- and ADP-dependent kinase","author_list":"Martín-Hurtado A, Contreras J, Sánchez-Wandelmer J, Zarzuela E, García F, Le Coq J, Boskovic J, Muñoz I, Gago F, Muñoz J, Isasa M, Plaza-Menacho I","journal":"Nature Communications","doi":"10.1038/s41467-026-69833-y","pmid":null,"published_date":"2026 Mar 06"},"status":"released","submitted_date":"2026-03-16","released_date":"2026-03-16"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVB5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVB5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVB5_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVB5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVB5.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger 4M","resolution":75.0},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"CCDC6-RET fusion protein variant c (K147M, Δ216-229)","short_name":"CCD6C-RET deltaCT","sequence":"MADSASESDTDGAGGNSSSSAAMQSSCSSTSGGGGGGGGGGGGGKSGGIV\r\nISPFRLEELTNRLASLQQENKVLKIELETYKLKCKALQEENRDLRKASVT\r\nIEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFHLKGRAGYTTVAVMMLK\r\nENASPSELRDLLSEFNVLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGS\r\nLRGFLRESRKVGPGYPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDL\r\nAARNILVAEGRKMKISDFGLSRDVYEEDSYVKRSQGRIPVKWMAIESLFD\r\nHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDN\r\nCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKRR","organism":"Homo sapiens","uniprot_code":"J7M8C2","uniprot_range_first":1,"uniprot_range_last":402,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MADSASESDTDGAGGNSSSSAAMQSSCSSTSGGGGGGGGGGGGGKSGGIVISPFRLEELT\nNRLASLQQENKVLKIELETYKLKCKALQEENRDLRKASVTIEDPKWEFPRKNLVLGKTLG\nEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQVNHPHVIKLY\nGACSQDGPLLLIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDL\nISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKRSQG\nRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRME\nRPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYLDLAASTPSDSLIYDD\nGLSEEETPLVDCNNAPLPRALPSTWIENKLYGRISHAFTRF","mw":43.085,"total_mw":86.17,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"CCDC6-RET fusion (J7M8C2) deltaCT (RET aa 713-1012, CCDC6 aa 1-101)"}],"buffer":{"name":"20 mM Tris, 500 mM NaCl, 1 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"CCDC6-RET fusion protein variant c","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Spanish National Cancer Research Centre (CNIO)","webpage":null}],"contributor_name":"INÉS","contributor_surname":"MUÑOZ","orcid":"https://orcid.org/0000-0001-6732-4059"}],"concentration_method":null,"concentration_unit":null,"date":"2023-09-18","storage_temperature":4.0,"cell_temperature":18.0,"exposure_time":3.0,"number_of_frames":600,"wavelength":0.1,"sample_detector_distance":3.7,"concentration_min":null,"concentration_max":5.0,"sample_volume":40.0,"flow_rate":0.16,"s_min":0.045,"s_max":3.397,"total_exposure_time":null,"seccolumn":48},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVB5_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDVB5_fit1_model1.pdb","model_mw":155.31,"bead_radius":5.0,"log":null,"symmetry":"P1","comment":null,"user":973}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVB5_fit1_fixed_fit_img.png","software":"FoXS","chi_square_value":2.222609,"p_value":0.0,"fit_residual_plot":"SASDVB5_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDVB5_fit1.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS experiments were conducted at the beamline B21 of the Diamond Light Source (Didcot, UK). CCDC6-RET∆CT (40 µL, 5 mg/mL) was injected at room temperature into a Superdex 200 Increase 3.2 column connected to an in-line Agilent 1200 HPLC system. The buffer used for the SEC column was the same protein buffer (20 mM Tris pH 7.5, 500 mM NaCl, 1 mM DTT). The eluted samples were exposed for 3s and 600 frames taken using an X-ray wavelength of 1 nm, and a sample to detector (Eiger 4M) distance of 3.7 m. The data covered a momentum transfer range of 0.032 < s < 3.4 nm-1. The frames recorded immediately before elution of the sample were subtracted from the protein scattering profiles. The Scåtter software package was used to analyze data, buffer-subtraction, scaling, merging and checking possible radiation damage of the samples.\r\nThe Rg value was calculated with the Guinier approximation assuming that at very small angles s < 1.3/Rg. The particle distance distribution, Dmax, was calculated from the scattering pattern with GNOM, and shape estimation was carried out with DAMMIF/DAMMIN, all these programs included in the ATSAS package.","tags":[],"intensity_unit":"arbitrary","experimental_mw":171.74,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":162.0,"porod_mw_error":null,"pddf_i0":0.07398,"pddf_i0_error":null,"guinier_i0":0.0745331,"guinier_i0_error":null,"pddf_rg":5.54,"pddf_rg_error":null,"guinier_rg":5.507,"guinier_rg_error":0.474,"pddf_dmax":19.5,"pddf_dmax_error":null,"porod_volume":259.7,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":42,"guinier_point_last":101,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVB5_datloglog_img.png","symmetry":null,"last_modified":"2026-03-16T10:35:21.105494+01:00","bragg_peak":[]}