{"code":"SASDVC5","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"The sequence–structure–function relationship of intrinsic ERα disorder","publication":{"title":"The sequence–structure–function relationship of intrinsic ERα disorder","author_list":"Du Z, Wang H, Luo S, Yun Z, Wu C, Yang W, Buck M, Zheng W, Hansen A, Kao H, Yang S","journal":"Nature","doi":"10.1038/s41586-024-08400-1","pmid":null,"published_date":"2025 Jan 08"},"status":"released","submitted_date":"2024-07-23","released_date":"2024-12-13"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVC5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVC5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVC5_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVC5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVC5.sascif","experiment":{"instrument":{"detector":{"type":"Duel detector","name":"Pilatus3 S 1M, Pilatus3 900 K","resolution":null},"name":"National Synchrotron Light Source II (NSLS-II)","city":"Upton, NY","country":"USA","beamline_name":"16-ID (LiX)","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Estrogen receptor","short_name":"ER-NTD","sequence":"SNAMTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRY","organism":"Homo sapiens","uniprot_code":"P03372","uniprot_range_first":1,"uniprot_range_last":184,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAY\nEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPF\nLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAK\nETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQAC\nRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKR\nSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINW\nAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG\nMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLD\nKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLL\nLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV","mw":20.175,"total_mw":20.175,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"SEC-SAXS data of phosphorylated estrogen receptor alpha (pSer118), N-terminal domain (NTD) at pH 7.4","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Cleveland, USA","full_name":"Case Western Reserve University","webpage":"https://case.edu/"}],"contributor_name":"Sichun","contributor_surname":"Yang","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2024-07-17","storage_temperature":8.0,"cell_temperature":4.0,"exposure_time":2.0,"number_of_frames":360,"wavelength":0.08189,"sample_detector_distance":4.003,"concentration_min":null,"concentration_max":2.0,"sample_volume":100.0,"flow_rate":0.35,"s_min":0.1,"s_max":2.5,"total_exposure_time":null,"seccolumn":54},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the phosphorylated N-terminal domain of estrogen receptor alpha (pSer118) in 20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP, pH 7.4 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II; Upton, NY, USA) using a Pilatus3 S 1M, Pilatus3 900 K detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 2 mg/ml was injected at a 0.35 ml/min flow rate onto a Cytiva Superdex 75 Increase 5/150 column at 4°C. 360 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":20.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":43.0,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":3.83022,"guinier_i0_error":null,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":3.635,"guinier_rg_error":0.607,"pddf_dmax":16.0,"pddf_dmax_error":null,"porod_volume":70.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":6,"guinier_point_last":30,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVC5_datloglog_img.png","symmetry":null,"last_modified":"2025-01-08T17:59:15.079200+01:00","bragg_peak":[]}