{"code":"SASDVE8","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Unraveling the molecular grammar and the structural transitions underlying the fibrillation of a viral fibrillogenic domain.","publication":{"title":"Unraveling the molecular grammar and the structural transitions underlying the fibrillation of a viral fibrillogenic domain.","author_list":"Gondelaud F, Leval J, Arora L, Walimbe A, Bignon C, Ptchelkine D, Brocca S, Mukhopadyay S, Longhi S","journal":"Protein Sci","doi":"10.1002/pro.70068","pmid":"39985377","published_date":"2025 Mar"},"status":"released","submitted_date":"2024-10-14","released_date":"2025-02-24"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVE8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVE8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVE8_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVE8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVE8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger 4M","resolution":75.0},"name":"SOLEIL","city":"Saint-Aubin","country":"France","beamline_name":"SWING","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Protein W (artificial PNT3 variant - high-kappa)","short_name":"PNT3 high K","sequence":"MSYYHHHHHHLESTSLYKKAGFTPTEEPPVIPEYYYGSGDDGDLSESPPEGNVNLDSIEIYTSDDEDENQLEYEDEFAESSSEVVIDTTPEDNDSINQEEVVGDPSDQGLKHPFPLGKFPKKKRTPRVRRKRS","organism":"Hendra virus (isolate Horse/Autralia/Hendra/1994)","uniprot_code":"P0C1C6","uniprot_range_first":200,"uniprot_range_last":310,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MDKLDLVNDGLDIIDFIQKNQKEIQKTYGRSSIQQPSTKDRTRAWEDFLQSTSGEHEQAE\r\nGGMPKNDGGTEGRNVEDLSSVTSSDGTIGQRVSNTRAWAEDPDDIQLDPMVTDVVYHDHG\r\nGECTGHGPSSSPERGWSYHMSGTHDGNVRAVPDTKVLPNAPKTTVPEEVREIDLIGLEDK\r\nFASAGLNPAAVPFVPKNQSTPTEEPPVIPEYYYGSGRRGDLSKSPPRGNVNLDSIKIYTS\r\nDDEDENQLEYEDEFAKSSSEVVIDTTPEDNDSINQEEVVGDPSDQGLEHPFPLGKFPEKE\r\nETPDVRRKDSLMQDSCKRGGVPKRLPMLSEEFECSGSDDPIIQELEREGSHPGGSLRLRE\r\nPPQSSGNSRNQPDRQLKTGDAASPGGVQRPGTPMPKSRIMPIKKGAQTRSLNMLGRKTCL\r\nGRRVVQPGMFADYPPTKKARVLLRRMSN","mw":15.198,"total_mw":15.198,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"Artificial variant of PNT3 with a segregated distribution of the opposite charges (High kappa). The protein construct used for SAXS contains a non-native polyhistitine tag and additional amino acids (MSYYHHHHHHLESTSLYKKAGF)."}],"buffer":{"name":"20 mM HEPES, 150 mM NaCl","concentration_unit":null,"comment":"HBS","additive":null,"concentration":null,"pka":null,"ph":7.2,"deuteration":null},"purity_method":null,"name":"Hendra virus protein P/V/W PNT3 high-K domain","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"13288 Marseille, France","full_name":"CNRS, AFMB UMR 7257 ","webpage":""}],"contributor_name":"Frank","contributor_surname":"Gondelaud","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2024-04-05","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":0.99,"number_of_frames":600,"wavelength":0.133,"sample_detector_distance":2.0,"concentration_min":null,"concentration_max":5.0,"sample_volume":50.0,"flow_rate":0.3,"s_min":0.089,"s_max":4.689,"total_exposure_time":null,"seccolumn":10},"fits":[{"models":[],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVE8_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":1.006,"p_value":0.1946,"fit_residual_plot":"SASDVE8_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDVE8_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_nHnJcis.log","software_version":null,"description":"EOM online was used."}],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":"50 µl were injected on a BioSec 3-300 at 0.3 ml/min in HBS","experiment_description":"Synchrotron SAXS data from solutions of Hendra virus protein P/V/W PNT3 high-K domain in 20 mM HEPES, 150 mM NaCl, pH 7.2 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.133 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 5 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 20°C. 600 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":23.3,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":33.6,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":1.10416,"guinier_i0_error":null,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":3.126,"guinier_rg_error":0.151,"pddf_dmax":14.7,"pddf_dmax_error":null,"porod_volume":53.8,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":51,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVE8_datloglog_img.png","symmetry":null,"last_modified":"2025-02-24T10:52:45.703208+01:00","bragg_peak":[]}