{"code":"SASDVM6","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Computational study of DPP8 and DPP9: fundamental insights and inhibitor design","publication":{"title":"Computational study of DPP8 and DPP9: fundamental insights and inhibitor design","author_list":"Olivier Beyens, Yann Sterckx","journal":"University of Antwerp PhD thesis","doi":null,"pmid":null,"published_date":"2024 Nov 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protein construct used for SAXS contains an additional C-terminal polyhistidine tag (HHHHHH)."}],"buffer":{"name":"25 mM HEPES, 150 mM NaCl, 2 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Dipeptidyl peptidase 9 (DPP9) with compound 42","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"University of 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GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of DPP9 with compound 42 in 25 mM HEPES, 150 mM NaCl, 2 mM DTT, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 12 mg/ml was injected at a 0.20 ml/min flow rate onto a Shodex KW404-4F column at 20°C. 750 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":199.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":171.0,"porod_mw_error":null,"pddf_i0":274.0,"pddf_i0_error":null,"guinier_i0":273.928,"guinier_i0_error":null,"pddf_rg":4.11,"pddf_rg_error":null,"guinier_rg":4.105,"guinier_rg_error":0.032,"pddf_dmax":12.5,"pddf_dmax_error":null,"porod_volume":274.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":3,"guinier_point_last":43,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVM6_datloglog_img.png","symmetry":null,"last_modified":"2025-01-14T14:58:30.315335+01:00","bragg_peak":[]}