{"code":"SASDVN4","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"A conformational fingerprint for amyloidogenic light chains","publication":{"title":"A conformational fingerprint for amyloidogenic light chains","author_list":"Paissoni C, Puri S, Broggini L, Sriramoju M, Maritan M, Russo R, Speranzini V, Ballabio F, Nuvolone M, Merlini G, Palladini G, Hsu S, Ricagno S, Camilloni C","journal":"eLife","doi":"10.7554/eLife.102002.3","pmid":null,"published_date":"2025 Mar 03"},"status":"released","submitted_date":"2024-07-12","released_date":"2025-02-24"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDVN4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVN4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVN4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVN4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVN4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVN4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVN4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 2M","resolution":0.172},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"point","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Immunoglobulin light chain H18","short_name":"H18","sequence":"VSVALGQIVTITCQGDSLRTYYASWYQQKPGQAPVLVIYNQDHRPSGIPDRFSGSSSGNTASLTIAGAQA\r\nNDEADYYCNSRDSSGNLVLFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVA\r\nWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT","organism":"Homo sapiens","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"","mw":21.448,"total_mw":42.896,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"20 mM TrisHCL, 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Immunoglobulin light chain H18","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Università degli studi di Milano","webpage":null}],"contributor_name":"Federico","contributor_surname":"Ballabio","orcid":"https://orcid.org/0000-0001-5702-3674"}],"concentration_method":null,"concentration_unit":null,"date":"2021-07-02","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":2.0,"number_of_frames":1400,"wavelength":0.099,"sample_detector_distance":2.98,"concentration_min":null,"concentration_max":6.7,"sample_volume":100.0,"flow_rate":0.1,"s_min":0.044,"s_max":5.205,"total_exposure_time":null,"seccolumn":47},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVN4_fit1_model1_img.png","software":"SwissModel","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":"SWISS-MODEL SERVER","model_data":"https://www.sasbdb.org/media/pdb_file/SASDVN4_fit1_model1.pdb","model_mw":43.59,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":959}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVN4_fit1_fixed_fit_img.png","software":"Pepsi-SAXS","chi_square_value":1.6071,"p_value":0.0,"fit_residual_plot":"SASDVN4_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDVN4_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/H18-H18_saxs.log","software_version":"3","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of immunoglobulin light chain H18 in 20 mM TrisHCL, 150 mM NaCl, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 3.0 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 6.7 mg/ml was injected at a 0.10 ml/min flow rate onto a Cytiva Superdex 200 Increase 10/300 column at 10°C. 1400 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":42.9,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":36.2,"porod_mw_error":null,"pddf_i0":19.66,"pddf_i0_error":null,"guinier_i0":19.7252,"guinier_i0_error":null,"pddf_rg":2.611,"pddf_rg_error":null,"guinier_rg":2.61,"guinier_rg_error":0.013,"pddf_dmax":8.2,"pddf_dmax_error":null,"porod_volume":57.9,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":17,"guinier_point_last":87,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVN4_datloglog_img.png","symmetry":null,"last_modified":"2025-02-24T14:43:25.100793+01:00","bragg_peak":[]}