{"code":"SASDVP4","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"A conformational fingerprint for amyloidogenic light chains","publication":{"title":"A conformational fingerprint for amyloidogenic light chains","author_list":"Paissoni C, Puri S, Broggini L, Sriramoju M, Maritan M, Russo R, Speranzini V, Ballabio F, Nuvolone M, Merlini G, Palladini G, Hsu S, Ricagno S, Camilloni C","journal":"eLife","doi":"10.7554/eLife.102002.3","pmid":null,"published_date":"2025 Mar 03"},"status":"released","submitted_date":"2024-07-12","released_date":"2025-02-24"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDVP4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVP4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVP4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVP4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVP4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVP4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVP4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 6M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Immunoglobulin light chain M7","short_name":"M7","sequence":"SSELTQDPAVSVALGQTVKITCQGDSLRMYYASWYQQKPAQAPVLVIYAEKNRPSGIPDRFSASSSGSTA\r\nSLTITGAQAEDEADYYCNSRDNSGDHLVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISD\r\nFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAP\r\nTECS","organism":"Homo sapiens","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"","mw":22.787,"total_mw":45.574,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"20 mM TrisHCL, 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Immunoglobulin light chain M7","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Università degli studi di Milano","webpage":null}],"contributor_name":"Federico","contributor_surname":"Ballabio","orcid":"https://orcid.org/0000-0001-5702-3674"}],"concentration_method":null,"concentration_unit":null,"date":"2018-05-12","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.124423,"sample_detector_distance":2.8,"concentration_min":null,"concentration_max":3.6,"sample_volume":null,"flow_rate":null,"s_min":0.024,"s_max":7.297,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVP4_fit1_model1_img.png","software":"MODELLER","pdb_link":[{"pdb_code":"5mvg","ext_code":"5MVG","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":"9.19","model_data":"https://www.sasbdb.org/media/pdb_file/SASDVP4_fit1_model1.pdb","model_mw":45.5,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":959}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVP4_fit1_fixed_fit_img.png","software":"Pepsi-SAXS","chi_square_value":1.2182,"p_value":0.0068,"fit_residual_plot":"SASDVP4_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDVP4_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/M7-M7_saxs.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of immunoglobulin light chain M7 in 20 mM TrisHCL, 150 mM NaCl, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.124423 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.60 mg/ml was measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":45.57,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":35.9,"porod_mw_error":null,"pddf_i0":774.9,"pddf_i0_error":null,"guinier_i0":779.26,"guinier_i0_error":null,"pddf_rg":2.535,"pddf_rg_error":null,"guinier_rg":2.53,"guinier_rg_error":0.035,"pddf_dmax":8.0,"pddf_dmax_error":null,"porod_volume":57.5,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":36,"guinier_point_last":150,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVP4_datloglog_img.png","symmetry":null,"last_modified":"2025-02-24T14:43:26.236867+01:00","bragg_peak":[]}