{"code":"SASDVQ7","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"The strand exchange domain of tumor suppressor PALB2 is intrinsically disordered and promotes oligomerization-dependent DNA compaction.","publication":{"title":"The strand exchange domain of tumor suppressor PALB2 is intrinsically disordered and promotes oligomerization-dependent DNA compaction.","author_list":"Kyriukha Y, Watkins MB, Redington JM, Chintalapati N, Ganti A, Dastvan R, Uversky VN, Hopkins JB, Pozzi N, Korolev S","journal":"iScience","doi":"10.1016/j.isci.2024.111259","pmid":"39584160","published_date":"2024 Dec 20"},"status":"released","submitted_date":"2024-10-05","released_date":"2024-12-23"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDVQ7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVQ7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVQ7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVQ7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVQ7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVQ7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVQ7.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger 4M","resolution":75.0},"name":"Cornell High Energy Synchrotron Source (CHESS)","city":"Ithaca, NY","country":"USA","beamline_name":"ID7A1 BioSAXS / HP-Bio Beamline","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Partner and localizer of BRCA2 (L24A)","short_name":"PALB2","sequence":"SMDEPPGKPLSCEEKEKLKEKLAFAKREYSKTLARLQRAQRAEKIKHSIKKTVEEQDCLSQQDLSPQLKHSEPKNKICVYDKLHIKTHLDEETGEKTSITLDVGPESFNPGDGPGGLPIQRTDDTQEHFPHRVSDPSGEQKQKLPSRRKKQQKRTFISQERDCVFGTDSLRLSGKRLKEQEEISSKNPARSPVTEWENLYFQS","organism":"Homo sapiens","uniprot_code":"Q86YC2","uniprot_range_first":1,"uniprot_range_last":194,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MDEPPGKPLSCEEKEKLKEKLAFLKREYSKTLARLQRAQRAEKIKHSIKKTVEEQDCLSQ\nQDLSPQLKHSEPKNKICVYDKLHIKTHLDEETGEKTSITLDVGPESFNPGDGPGGLPIQR\nTDDTQEHFPHRVSDPSGEQKQKLPSRRKKQQKRTFISQERDCVFGTDSLRLSGKRLKEQE\nEISSKNPARSPVTEIRTHLLSLKSELPDSPEPVTEINEDSVLIPPTAQPEKGVDTFLRRP\nNFTRATTVPLQTLSDSGSSQHLEHIPPKGSSELTTHDLKNIRFTSPVSLEAQGKKMTVST\nDNLLVNKAISKSGQLPTSSNLEANISCSLNELTYNNLPANENQNLKEQNQTEKSLKSPSD\nTLDGRNENLQESEILSQPKSLSLEATSPLSAEKHSCTVPEGLLFPAEYYVRTTRSMSNCQ\nRKVAVEAVIQSHLDVKKKGFKNKNKDASKNLNLSNEETDQSEIRMSGTCTGQPSSRTSQK\nLLSLTKVSSPAGPTEDNDLSRKAVAQAPGRRYTGKRKSACTPASDHCEPLLPTSSLSIVN\nRSKEEVTSHKYQHEKLFIQVKGKKSRHQKEDSLSWSNSAYLSLDDDAFTAPFHRDGMLSL\nKQLLSFLSITDFQLPDEDFGPLKLEKVKSCSEKPVEPFESKMFGERHLKEGSCIFPEELS\nPKRMDTEMEDLEEDLIVLPGKSHPKRPNSQSQHTKTGLSSSILLYTPLNTVAPDDNDRPT\nTDMCSPAFPILGTTPAFGPQGSYEKASTEVAGRTCCTPQLAHLKDSVCLASDTKQFDSSG\nSPAKPHTTLQVSGRQGQPTCDCDSVPPGTPPPIESFTFKENQLCRNTCQELHKHSVEQTE\nTAELPASDSINPGNLQLVSELKNPSGSCSVDVSAMFWERAGCKEPCIITACEDVVSLWKA\nLDAWQWEKLYTWHFAEVPVLQIVPVPDVYNLVCVALGNLEIREIRALFCSSDDESEKQVL\nLKSGNIKAVLGLTKRRLVSSSGTLSDQQVEVMTFAEDGGGKENQFLMPPEETILTFAEVQ\nGMQEALLGTTIMNNIVIWNLKTGQLLKKMHIDDSYQASVCHKAYSEMGLLFIVLSHPCAK\nESESLRSPVFQLIVINPKTTLSVGVMLYCLPPGQAGRFLEGDVKDHCAAAILTSGTIAIW\nDLLLGQCTALLPPVSDQHWSFVKWSGTDSHLLAGQKDGNIFVYHYS","mw":23.325,"total_mw":23.325,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"L24A monomeric mutant of PABL2. The protein construct used for SAXS contains a non-native amino acid C-terminal extension (WENLYFQS)."}],"buffer":{"name":"20 mM HEPES, 160 mM NaCl, 0.5 mM TCEP pH 7.5","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"PABL2 (Partner and localizer of BRCA2) L24A mutant","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Illinois Institute of Technology","webpage":null}],"contributor_name":"Maxwell","contributor_surname":"Watkins","orcid":"https://orcid.org/0000-0003-4559-2049"}],"concentration_method":null,"concentration_unit":null,"date":"2023-12-09","storage_temperature":-20.0,"cell_temperature":23.0,"exposure_time":1.0,"number_of_frames":2199,"wavelength":0.1097,"sample_detector_distance":1.78,"concentration_min":null,"concentration_max":5.1,"sample_volume":null,"flow_rate":null,"s_min":0.083,"s_max":4.432,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVQ7_fit1_model1_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDVQ7_fit1_model1.pdb","model_mw":23.31,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_bqXK9dm.log","symmetry":null,"comment":"Model generated by SASSIE Monte Carlo, sub-ensemble selected by GAJOE (EOM/Atsas)","user":941},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVQ7_fit1_model2_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDVQ7_fit1_model2.pdb","model_mw":23.31,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_TvG1vcw.log","symmetry":null,"comment":"Model generated by SASSIE Monte Carlo, sub-ensemble selected by GAJOE (EOM/Atsas)","user":941},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVQ7_fit1_model3_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDVQ7_fit1_model3.pdb","model_mw":23.31,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_lhNySOK.log","symmetry":null,"comment":"Model generated by SASSIE Monte Carlo, sub-ensemble selected by GAJOE (EOM/Atsas)","user":941},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVQ7_fit1_model4_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDVQ7_fit1_model4.pdb","model_mw":23.31,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_ELgCP9x.log","symmetry":null,"comment":"Model generated by SASSIE Monte Carlo, sub-ensemble selected by GAJOE (EOM/Atsas)","user":941},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVQ7_fit1_model5_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDVQ7_fit1_model5.pdb","model_mw":23.31,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_BcEA3zQ.log","symmetry":null,"comment":null,"user":941},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVQ7_fit1_model6_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDVQ7_fit1_model6.pdb","model_mw":23.31,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_x0T5g9y.log","symmetry":null,"comment":"Model generated by SASSIE Monte Carlo, sub-ensemble selected by GAJOE (EOM/Atsas)","user":941}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVQ7_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":1.395,"p_value":0.0017,"fit_residual_plot":"SASDVQ7_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDVQ7_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_UhvSbbF.log","software_version":"3.0","description":"Structural pool was generated using SASSIE monomer monte carlo, with residues 10-40 designated as non-flexible residues and used for structural alignment. The sub-ensemble was fit using GAJOE."}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"REGALS-separated main component profile from SEC-SAXS experiment measuring PABL2 L24A mutant. NOTE: There is a significant inconsistency between the calculated MW from sequence of the monomer (23 kDa) and the quoted experimental MW (50 kDa). CAUTION: The X-ray exposure time is unlikely to be 2199 seconds.","experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":49.6,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":5.136,"pddf_i0_error":0.05,"guinier_i0":5.09168,"guinier_i0_error":null,"pddf_rg":5.65,"pddf_rg_error":0.08,"guinier_rg":5.23,"guinier_rg_error":0.888,"pddf_dmax":21.6,"pddf_dmax_error":null,"porod_volume":127.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":21,"guinier_point_last":67,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVQ7_datloglog_img.png","symmetry":null,"last_modified":"2024-12-23T14:34:33.185010+01:00","bragg_peak":[]}