{"code":"SASDVS5","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation.","publication":{"title":"Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation.","author_list":"Lin TC, Shih O, Tsai TY, Yeh YQ, Liao KF, Mansel BW, Shiu YJ, Chang CF, Su AC, Chen YR, Jeng US","journal":"IUCrJ","doi":"10.1107/S2052252524006341","pmid":"39120045","published_date":"2024 Sep 1"},"status":"released","submitted_date":"2024-08-01","released_date":"2024-08-07"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDVS5.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVS5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVS5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVS5_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVS5_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVS5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVS5.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger X 1M & 9M","resolution":0.001},"name":"NSRRC","city":"Hsinchu","country":"Taiwan","beamline_name":"TPS13A","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Isoform Short of Small EDRK-rich factor 1","short_name":null,"sequence":"MARGNQRELARQKNMKKTQEISKGKRKEDSLTASQRKQRDSEIMQEKQKAANEKKSMQTREK","organism":"Homo sapiens","uniprot_code":"O75920","uniprot_range_first":1,"uniprot_range_last":62,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MARGNQRELARQKNMKKTQEISKGKRKEDSLTASQRKQRDSEIMQEKQKAANEKKSMQTR\nEK","mw":7.336,"total_mw":7.336,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""},{"long_name":"HTT3","short_name":null,"sequence":"MATPEKPMKAFESPKSFPQQPQQQPQQPQQQYK","organism":"synthetic construct","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"","mw":3.887,"total_mw":3.887,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"Sodium phosphate buffer","concentration_unit":null,"comment":"(PB) solution (containing 480 μL of 10 mM PB, pH 7.4, 16.5 μL of 100 mM NaOH, and 10 μL of 1% TFA)","additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Small EDRK-rich factor 1 (SERF1a) bound to HTT-3 peptide","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Hsinchu City, Taiwan","full_name":"National Synchrotron Radiation Research Center","webpage":"https://www.nsrrc.org.tw/"}],"contributor_name":"Orion","contributor_surname":"Shih","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2021-05-20","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":2.0,"number_of_frames":5,"wavelength":0.08265,"sample_detector_distance":2.5,"concentration_min":0.5,"concentration_max":0.8,"sample_volume":70.0,"flow_rate":0.35,"s_min":0.126,"s_max":2.953,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVS5_fit1_model1_img.png","software":"Rosetta","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDVS5_fit1_model1.pdb","model_mw":11.26,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":216}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVS5_fit1_fixed_fit_img.png","software":"CRYSOL","chi_square_value":2.2084,"p_value":0.2523,"fit_residual_plot":"SASDVS5_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDVS5_fit1.fit","fit_log":null,"software_version":"3.2.1","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The sodium phosphate buffer solution contains 480 μL of 10 mM PB, pH 7.4, 16.5 μL of 100 mM NaOH, and 10 μL of 1% trifluoroacetic acid (TFA). CAUTION: Severe steric clashes noted in the model.","experiment_description":"Synchrotron SAXS data from solutions of small EDRK-rich factor 1 (SERF1a) bound to HTT-3 peptide in sodium phosphate buffer, pH 7.4 were collected on the TPS13A beam line at the NSRRC (Hsinchu, Taiwan) using a Eiger X 1M & 9M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.08265 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 0.8 mg/ml were measured at 10°C. Five successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":"1/cm","experimental_mw":6.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.008304,"pddf_i0_error":null,"guinier_i0":0.00860341,"guinier_i0_error":null,"pddf_rg":2.21,"pddf_rg_error":null,"guinier_rg":2.3,"guinier_rg_error":0.341,"pddf_dmax":7.16,"pddf_dmax_error":null,"porod_volume":8.8,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":35,"guinier_point_last":119,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVS5_datloglog_img.png","symmetry":null,"last_modified":"2024-08-18T16:49:41.246068+02:00","bragg_peak":[]}