{"code":"SASDVT6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation.","publication":{"title":"Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation.","author_list":"Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkaf502","pmid":"40503686","published_date":"2025 Jun 6"},"status":"released","submitted_date":"2023-09-21","released_date":"2025-02-10"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDVT6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVT6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVT6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVT6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVT6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVT6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVT6.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Nucleoprotein (L223P, L227P, L230P)","short_name":"N-protein LP mutant","sequence":"GPSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLPLDRPNQPESKMSGKGQQQQGQTWSHPQFEK","organism":"Severe acute respiratory syndrome coronavirus 2","uniprot_code":"P0DTC9","uniprot_range_first":176,"uniprot_range_last":245,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHG\nKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAG\nLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGS\nQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQ\nQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH\nWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAY\nKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA","mw":8.325,"total_mw":8.325,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"Strep(II)-tagged N-protein 176-245, L223P, L227P, L230P triple mutant. The protein construct used for SAXS contains an additional two amino acids at the N-terminus (GP) as well as the VWSHPQFEK C-terminal tag."}],"buffer":{"name":"100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245) L223P, L227P and L230P triple mutant: 1667 µM","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Oeiras, Portugal","full_name":"Instituto de Tecnologia Química e Biológica António Xavier, Universidade de Lisboa","webpage":"http://www.itqb.unl.pt"}],"contributor_name":"Guillem","contributor_surname":"Hernandez","orcid":"https://orcid.org/0000-0003-4438-5611"}],"concentration_method":null,"concentration_unit":null,"date":"2023-01-29","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":2.0,"number_of_frames":10,"wavelength":0.0999,"sample_detector_distance":2.8,"concentration_min":null,"concentration_max":14.0,"sample_volume":null,"flow_rate":null,"s_min":0.229,"s_max":5.21,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the SARS-CoV-2 N-protein inter-domain linker triple mutant (IDL, amino acids 176-245; L223P, L227P and L230P) in 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.0999 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 14.00 mg/ml was measured at 10°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":8.5,"experimental_mw_error":null,"guinier_i0_mw":12.3,"guinier_i0_mw_error":0.2,"porod_mw":null,"porod_mw_error":null,"pddf_i0":69.41,"pddf_i0_error":null,"guinier_i0":69.5127,"guinier_i0_error":null,"pddf_rg":2.85,"pddf_rg_error":0.02,"guinier_rg":2.751,"guinier_rg_error":2.995,"pddf_dmax":12.0,"pddf_dmax_error":0.5,"porod_volume":13.9,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":6,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVT6_datloglog_img.png","symmetry":null,"last_modified":"2025-06-16T15:30:17.918367+02:00","bragg_peak":[]}