{"code":"SASDVV5","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1.","publication":{"title":"The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1.","author_list":"Crespo I, Malfois M, Rienstra J, Tarrés-Solé A, van den Berg W, Weijers D, Boer DR","journal":"Structure","doi":"10.1016/j.str.2025.02.006","pmid":"40086441","published_date":"2025 Mar 7"},"status":"released","submitted_date":"2024-08-01","released_date":"2024-12-09"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDVV5.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVV5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVV5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVV5_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVV5_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVV5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVV5.sascif","experiment":{"instrument":{"detector":{"type":"CCD","name":"ADSC Quantum 210r","resolution":null},"name":"ALBA","city":"Cerdanyola del Vallès, Barcelona","country":"Spain","beamline_name":"BL11 - NCD","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Auxin response factor 1","short_name":"AtARF1","sequence":"MAASNHSSGKPGGVLSDALCRELWHACAGPLVTLPREGERVYYFPEGHME\r\nQLEASMHQGLEQQMPSFNLPSKILCKVINIQRRAEPETDEVYAQITLLPE\r\nLDQSEPTSPDAPVQEPEKCTVHSFCKTLTASDTSTHGGFSVLRRHADDCL\r\nPPLDMSQQPPWQELVATDLHNSEWHFRHIFRGQPRRHLLTTGWSVFVSSK\r\nKLVAGDAFIFLRGENEELRVGVRRHMRQQTNIPSSVISSHSMHIGVLATA\r\nAHAITTGTIFSVFYKPRTSRSEFIVSVNRYLEAKTQKLSVGMRFKMRFEG\r\nEEAPEKRFSGTIVGVQENKSSVWHDSEWRSLKVQWDEPSSVFRPERVSPW\r\nELEPLN","organism":"Arabidopsis thaliana","uniprot_code":"Q8L7G0","uniprot_range_first":1,"uniprot_range_last":355,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MAASNHSSGKPGGVLSDALCRELWHACAGPLVTLPREGERVYYFPEGHMEQLEASMHQGL\nEQQMPSFNLPSKILCKVINIQRRAEPETDEVYAQITLLPELDQSEPTSPDAPVQEPEKCT\nVHSFCKTLTASDTSTHGGFSVLRRHADDCLPPLDMSQQPPWQELVATDLHNSEWHFRHIF\nRGQPRRHLLTTGWSVFVSSKKLVAGDAFIFLRGENEELRVGVRRHMRQQTNIPSSVISSH\nSMHIGVLATAAHAITTGTIFSVFYKPRTSRSEFIVSVNRYLEAKTQKLSVGMRFKMRFEG\nEEAPEKRFSGTIVGVQENKSSVWHDSEWRSLKVQWDEPSSVFRPERVSPWELEPLVANST\nPSSQPQPPQRNKRPRPPGLPSPATGPSGPVTPDGVWKSPADTPSSVPLFSPPAKAATFGH\nGGNKSFGVSIGSAFWPTNADSAAESFASAFNNESTEKKQTNGNVCRLFGFELVENVNVDE\nCFSAASVSGAVAVDQPVPSNEFDSGQQSEPLNINQSDIPSGSGDPEKSSLRSPQESQSRQ\nIRSCTKVHMQGSAVGRAIDLTRSECYEDLFKKLEEMFDIKGELLESTKKWQVVYTDDEDD\nMMMVGDDPWNEFCGMVRKIFIYTPEEVKKLSPKNKLAVNARMQLKADAEENGNTEGRSSS\nMAGSR","mw":40.34,"total_mw":80.68,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"The protein construct used for SAXS encompasses the N-terminal region of the full length protein and contains an additional non-native asparagine at the C-terminus (N356)."},{"long_name":"High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing","short_name":"HA7","sequence":"T TGTCGG CGATTCG CCGACA A","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"DNA","uniprot_sequence":"","mw":6.502,"total_mw":13.004,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Arabidopsis thaliana Auxin Response Factor 1 in complex with high affinity DNA","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[],"concentration_method":null,"concentration_unit":null,"date":"2019-12-03","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":0.5,"number_of_frames":20,"wavelength":0.1,"sample_detector_distance":2.56,"concentration_min":null,"concentration_max":3.5,"sample_volume":null,"flow_rate":null,"s_min":0.08,"s_max":5.82,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"CAUTION: Dmax appears underestimated.","experiment_description":"Synchrotron SAXS data from solutions of A. thaliana auxin response factor 1 in complex with high affinity DNA in 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH 8 were collected on the BL11 - NCD beam line at ALBA (Cerdanyola del Vallès, Barcelona, Spain) using a ADSC Quantum 210r detector at a sample-detector distance of 2.6 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.50 mg/ml was measured at 20°C. 20 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":94.225,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":90.0,"porod_mw_error":null,"pddf_i0":9.22,"pddf_i0_error":null,"guinier_i0":9.34434,"guinier_i0_error":null,"pddf_rg":3.535,"pddf_rg_error":null,"guinier_rg":3.56,"guinier_rg_error":0.019,"pddf_dmax":10.49,"pddf_dmax_error":null,"porod_volume":136.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":47,"guinier_point_last":99,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVV5_datloglog_img.png","symmetry":null,"last_modified":"2025-03-20T11:11:01.853034+01:00","bragg_peak":[]}