{"code":"SASDVV7","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation.","publication":{"title":"A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation.","author_list":"Nguyen BV, Özden C, Dong K, Koc OC, Torres-Ocampo AP, Dziedzic N, Flaherty D, Huang J, Sankara S, Abromson NL, Tomchick DR, Fissore RA, Chen J, Garman SC, Stratton MM","journal":"Nat Commun","doi":"10.1038/s41467-025-63249-w","pmid":"41006217","published_date":"2025 Sep 26"},"status":"released","submitted_date":"2024-10-05","released_date":"2025-09-03"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDVV7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVV7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVV7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVV7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVV7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVV7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVV7.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 2M","resolution":172.0},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329)","short_name":"CaMKII Exon 18","sequence":"TRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH","organism":"Homo sapiens","uniprot_code":"Q9UQM7","uniprot_range_first":7,"uniprot_range_last":478,"oligomerization":"dodecamer","molecular_type":"protein","uniprot_sequence":"MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLERE\nARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQIL\nEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYL\nSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTV\nTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLK\nGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEA\nISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIH\nLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH","mw":52.166,"total_mw":625.992,"number_molecules":12,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"The linker region only contains exon 18."}],"buffer":{"name":"25 mM tris-HCl pH 8.8, 250 mM KCl, 1 mM TCEP, 2% glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":null,"deuteration":null},"purity_method":null,"name":"Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 18 as the linker region","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"university of massachusetts amherst","webpage":null}],"contributor_name":"Bao","contributor_surname":"Nguyen Viet","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2023-04-17","storage_temperature":-20.0,"cell_temperature":7.0,"exposure_time":3.0,"number_of_frames":600,"wavelength":0.1127,"sample_detector_distance":2.1,"concentration_min":6.0,"concentration_max":6.0,"sample_volume":55.0,"flow_rate":0.65,"s_min":0.114,"s_max":4.729,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVV7_fit1_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"2.3i","model_data":"https://www.sasbdb.org/media/pdb_file/SASDVV7_fit1_model1.cif","model_mw":null,"bead_radius":null,"log":null,"symmetry":"P62","comment":null,"user":1133}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVV7_fit1_fixed_fit_img.png","software":"GASBOR","chi_square_value":1.31,"p_value":0.0,"fit_residual_plot":"SASDVV7_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDVV7_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/Exon18.log","software_version":"2.3i","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"6.2.4","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 18 as the linker region in 25 mM tris-HCl pH 8.8, 250 mM KCl, 1 mM TCEP, 2% glycerol were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 6 and 6 mg/ml were measured at 7°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":"arbitrary","experimental_mw":542.3,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":15890.0,"pddf_i0_error":null,"guinier_i0":15761.0,"guinier_i0_error":null,"pddf_rg":6.06,"pddf_rg_error":null,"guinier_rg":5.996,"guinier_rg_error":0.634,"pddf_dmax":19.0,"pddf_dmax_error":null,"porod_volume":1080.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":20,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVV7_datloglog_img.png","symmetry":null,"last_modified":"2025-10-07T11:35:26.599738+02:00","bragg_peak":[]}