{"code":"SASDVX2","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2.","publication":{"title":"Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2.","author_list":"Mertinkus KR, Oxenfarth A, Richter C, Wacker A, Mata CP, Carazo JM, Schlundt A, Schwalbe H","journal":"J Am Chem Soc","doi":"10.1021/jacs.4c08406","pmid":"39442924","published_date":"2024 Nov 6"},"status":"released","submitted_date":"2024-07-01","released_date":"2024-09-30"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDVX2.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDVX2.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVX2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVX2_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVX2_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVX2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDVX2.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"full stem-loop 5 of SARS-CoV-2 5'genomic end","short_name":"5_SL5","sequence":"gGU CGU UGA CAG GAC ACG AGU AAC UCG UCU AUC UUC UGC AGG CUG CUU ACG GUU UCG UCC GUG UUG CAG CCG AUC AUC AGC ACA UCU AGG UUU CGU CCG GGU GUG ACC GAA AGG UAA GAU GGA GAG CCU UGU CCC UGG UUU CAA CGA GAA","organism":"Severe acute respiratory syndrome coronavirus 2","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"RNA","uniprot_sequence":"","mw":48.3,"total_mw":48.3,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"contains additional 5' artificial G for efficient IVT"}],"buffer":{"name":"25 mM potassium phosphate, 50 mM KCl","concentration_unit":null,"comment":"also used with 10 mM Mg2+ or 10 mM Mg2+/5 mM GdnCl","additive":null,"concentration":null,"pka":null,"ph":6.2,"deuteration":null},"purity_method":null,"name":"Full-length SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Goethe University Frankfurt, Dept. Biosciences","webpage":null}],"contributor_name":"Andreas","contributor_surname":"Schlundt","orcid":"https://orcid.org/0000-0003-2254-7560"}],"concentration_method":null,"concentration_unit":null,"date":"2020-08-07","storage_temperature":20.0,"cell_temperature":10.0,"exposure_time":0.095,"number_of_frames":40,"wavelength":0.124028,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":1.0,"sample_volume":null,"flow_rate":null,"s_min":0.025,"s_max":7.347,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVX2_fit1_model1_img.png","software":"PyMOL","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDVX2_fit1_model1.pdb","model_mw":44.88,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":741}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVX2_fit1_fixed_fit_img.png","software":"CRYSOL","chi_square_value":6.974,"p_value":0.0,"fit_residual_plot":"SASDVX2_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDVX2_fit1.fit","fit_log":null,"software_version":null,"description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDVX2_fit2_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDVX2_fit2_model1.pdb","model_mw":null,"bead_radius":null,"log":null,"symmetry":"P1","comment":null,"user":741}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVX2_fit2_fixed_fit_img.png","software":"DAMMIF","chi_square_value":1.098,"p_value":0.0023,"fit_residual_plot":"SASDVX2_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDVX2_fit2.fir","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":49.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":19930.0,"pddf_i0_error":null,"guinier_i0":19868.5,"guinier_i0_error":null,"pddf_rg":4.425,"pddf_rg_error":null,"guinier_rg":4.32,"guinier_rg_error":0.02,"pddf_dmax":13.8,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":33,"guinier_point_last":99,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDVX2_datloglog_img.png","symmetry":null,"last_modified":"2024-11-13T14:41:48.755235+01:00","bragg_peak":[]}