{"code":"SASDW27","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"The extracellular domain of SaNSrFP binds bacitracin and allows the identification of new members of the BceAB transporter family","publication":{"title":"The extracellular domain of SaNSrFP binds bacitracin and allows the identification of new members of the BceAB transporter family","author_list":"Mammen C, Gottstein J, Cea P, Tantsur K, Reiners J, Bonus M, Gohlke H, Smits S","journal":"Frontiers in Microbiology","doi":"10.3389/fmicb.2025.1662803","pmid":null,"published_date":"2025 Sep 17"},"status":"released","submitted_date":"2025-02-04","released_date":"2025-09-01"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDW27.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDW27.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW27_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW27_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDW27_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW27_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDW27.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 2M","resolution":0.172},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"point","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"ABC transporter ATP-binding protein","short_name":"SaNsrF","sequence":"MGHHHHHHHHHHSSGHIEGRHMLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESGSGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLSVRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLITNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKDGRIFHQLYRGNKNNSEFNKDISLTMSAILGGE","organism":"Streptococcus agalactiae","uniprot_code":"X5KGL2","uniprot_range_first":1,"uniprot_range_last":250,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESGSGKTTLLNILATLEKP\nTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLSVRDNIYLPLVLDRKRYKE\nMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLITNPQILLADEPTAALDYRNS\nEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKDGRIFHQLYRGNKNNSEFNKDIS\nLTMSAILGGE","mw":30.895,"total_mw":61.79,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"ATP-binding protein, part from the whole SaNsrFP complex\r\nAmino acid 1-21 belongs to an artificial His-tag and a thrombin cleavage site."},{"long_name":"ABC transporter, permease protein","short_name":"SaNsrP","sequence":"MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVGRDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVASLELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIFLTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAPALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMKQNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFEEKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDFRRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGLIIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVGNISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQEVGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSGLTVLAISIIYFIIYRITSRTYYHIIER","organism":"Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)","uniprot_code":"Q8DZX0","uniprot_range_first":1,"uniprot_range_last":651,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVGRDMGTAATVLFLGVIV\nLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVASLELFIIYIFLISIGSLFS\nAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIFLTLEVPVIRHVHLSSPLSLF\nRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAPALAVIYRFFFAVLLVIAGTYLF\nYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMKQNAVGLASITLLAVMALVTIATTV\nSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFEEKILKKLGKSSKEAITYNQTMISMPV\nSQSSELNITSKNVKHVDITKTGFMYLITQNDFRRLGHQLPKLKDNQVAYFVQKGDSRLKK\nINLLGNKFDVVKNLKEAYVPETTNTYNPGLIIFANNKQIDNIRKAYLPYTKNINTFPKTF\nKAYLDLNSQEINSISKNDIIEVDGKYVGNISTKQSFLKEGYQMFGGLLFTGFLLGISFLL\nGIALIVYYKQYSEGHEDKRSYRILQEVGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVA\nIPMLKQMLLVFGVLNSTIVYVVSGLTVLAISIIYFIIYRITSRTYYHIIER","mw":74.016,"total_mw":74.016,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"50 mM Tris, 300 mM NaCl, 10 % Glycerol, 0.005 % LMNG,","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"ABC transporter ATP-binding protein - 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