{"code":"SASDW33","status":"Published","type_of_curve":"Extrapolated to infinite dilution","angular_unit":"1/A","project":{"title":"Cryo-EM led analysis of open and closed conformations of Chagas vaccine candidate TcPOP.","publication":{"title":"Cryo-EM led analysis of open and closed conformations of Chagas vaccine candidate TcPOP.","author_list":"Batra S, Olmo F, Ragan TJ, Kaplan M, Calvaresi V, Frank AM, Lancey C, Assadipapari M, Ying C, Struwe WB, Hesketh EL, Kelly JM, Barfod L, Campeotto I","journal":"Nat Commun","doi":"10.1038/s41467-025-62068-3","pmid":"40764299","published_date":"2025 Aug 5"},"status":"released","submitted_date":"2024-12-07","released_date":"2025-08-07"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDW33.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDW33.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW33_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW33_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDW33_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW33_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDW33.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger 4M","resolution":75.0},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Prolyl endopeptidase","short_name":null,"sequence":">sp|Q71MD6|Q71MD6_TRYCR 80 kDa prolyl oligopeptidase\r\nMRSVYPLARRSMAAYTMHNMTVPEPYDYLEDPENPETKTFVNEQNAFFEEYFASEAELRKKIFESISNSQ\r\nDYPRTSNPSYINGHYYYYHNSGLQNQSVLMRAMSLTDTAPSIFLDPNSMSSDGTTALKATAWSEDESMLA\r\nYSLSDKGSDWQRIHVRRADTVEDTSDVIEWAKFTAIAWWHNLGFFYTRYPALQGDVDKGAETDAAQDAFI\r\nCFHRIGRPQDEDVVILSVPEHPQWNMGASVSDCHSYVIVVLFDGCEPHNLVWVAELPSVEKGLGSEPLVF\r\nKKLVNEFAGRYTYLGNEGSTFYFVTTRDAPRKKIVSIDIHTGQETVIVEQQRSVLSQAALVKKTLLLAYL\r\nEDVKDVFYYCRLEDPTLNAIPLPIGTITSFFSDRKKDFVSFKITSFLLPGRSFFLDINDPQSSLRVFKDD\r\nTVEGLLVDDFVTEQTFYNSSDGVRIPMFIVYRKGSVSSESPLLLYGYGGFNIPLTPAFSSSRMVFLRDLG\r\nGVLAVLNIRGGGEYGEEWHDAGRRACKQNCFTDFIEGAKFLHRQGYGSPQTTAIMGGSNGGLLVAAVANQ\r\nAPELFRCVVCRVGVLDMYKFHKFTIGHAWKSDYGDPEKEEDFRVLQQYSPLHNIKSGIKYPAILVVTGDH\r\nDDRVVPLHSLKYVATLQHMNPNEGGPFLARIEVAAGHGAGKPTSKILREAGDIYTFIAKNINASWKE","organism":"Trypanosoma cruzi","uniprot_code":"Q71MD6","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MRSVYPLARRSMAAYTMHNMTVPEPYDYLEDPENPETKTFVNEQNAFFEEYFASEAELRK\nKIFESISNSQDYPRTSNPSYINGHYYYYHNSGLQNQSVLMRAMSLTDTAPSIFLDPNSMS\nSDGTTALKATAWSEDESMLAYSLSDKGSDWQRIHVRRADTVEDTSDVIEWAKFTAIAWWH\nNLGFFYTRYPALQGDVDKGAETDAAQDAFICFHRIGRPQDEDVVILSVPEHPQWNMGASV\nSDCHSYVIVVLFDGCEPHNLVWVAELPSVEKGLGSEPLVFKKLVNEFAGRYTYLGNEGST\nFYFVTTRDAPRKKIVSIDIHTGQETVIVEQQRSVLSQAALVKKTLLLAYLEDVKDVFYYC\nRLEDPTLNAIPLPIGTITSFFSDRKKDFVSFKITSFLLPGRSFFLDINDPQSSLRVFKDD\nTVEGLLVDDFVTEQTFYNSSDGVRIPMFIVYRKGSVSSESPLLLYGYGGFNIPLTPAFSS\nSRMVFLRDLGGVLAVLNIRGGGEYGEEWHDAGRRACKQNCFTDFIEGAKFLHRQGYGSPQ\nTTAIMGGSNGGLLVAAVANQAPELFRCVVCRVGVLDMYKFHKFTIGHAWKSDYGDPEKEE\nDFRVLQQYSPLHNIKSGIKYPAILVVTGDHDDRVVPLHSLKYVATLQHMNPNEGGPFLAR\nIEVAAGHGAGKPTSKILREAGDIYTFIAKNINASWKE","mw":78.335,"total_mw":78.335,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"80 kDa prolyl oligopeptidase"}],"buffer":{"name":"20 mM HEPES, 150 mM NaCl","concentration_unit":null,"comment":"Purification buffer","additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"STATIC-SAXS of Trypanosoma cruzi Prolyl Oligopepetidase (TcPOP)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"university of nottingham","webpage":null}],"contributor_name":"Ivan","contributor_surname":"Campeotto","orcid":"https://orcid.org/0000-0002-0814-619X"}],"concentration_method":null,"concentration_unit":null,"date":"2023-04-24","storage_temperature":15.0,"cell_temperature":15.0,"exposure_time":1.0,"number_of_frames":21,"wavelength":0.09464,"sample_detector_distance":3.6856,"concentration_min":0.3,"concentration_max":6.0,"sample_volume":50.0,"flow_rate":0.08,"s_min":0.045,"s_max":3.4,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"BioXTAS RAW","pddf_software_version":null,"i0_calibration_standard":null,"description":"CAUTION! Data are severely over subtracted. CAUTION! Severe aggregation. CAUTION! The Rg, I(0), particle size/volume, molecular weight, p(r) and any other structural parameters derived from the data are undefined/uninterpretable.","experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":100.0,"experimental_mw_error":null,"guinier_i0_mw":105.5,"guinier_i0_mw_error":null,"porod_mw":106.0,"porod_mw_error":null,"pddf_i0":0.01332995,"pddf_i0_error":null,"guinier_i0":0.0417137,"guinier_i0_error":null,"pddf_rg":0.0,"pddf_rg_error":null,"guinier_rg":3.81,"guinier_rg_error":0.615,"pddf_dmax":0.03,"pddf_dmax_error":null,"porod_volume":170.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":155,"guinier_point_last":232,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDW33_datloglog_img.png","symmetry":null,"last_modified":"2025-08-07T13:57:51.376271+02:00","bragg_peak":[]}