{"code":"SASDW63","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Early events in G-quadruplex folding captured by time-resolved small-angle X-ray scattering.","publication":{"title":"Early events in G-quadruplex folding captured by time-resolved small-angle X-ray scattering.","author_list":"Monsen RC, Sabo TM, Gray R, Hopkins JB, Chaires JB","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkaf043","pmid":"39883009","published_date":"2025 Jan 24"},"status":"released","submitted_date":"2024-11-28","released_date":"2025-02-10"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDW63.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDW63.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW63_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW63_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDW63_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW63_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDW63.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 1M","resolution":0.172},"name":"Advanced Photon Source (APS), Argonne National Laboratory","city":"Lemont, IL","country":"USA","beamline_name":"BioCAT 18ID","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Telomere DNA G-quadruplex Hybrid-1 form","short_name":"2GKU","sequence":"TTGGGTTAGGGTTAGGGTTAGGGA","organism":"synthetic construct","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"DNA","uniprot_sequence":"","mw":7.655,"total_mw":7.655,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"Telomere DNA G-quadruplex in hybrid-1 form"}],"buffer":{"name":"6 mM Li2HPO4, 2 mM LiH2PO4, 1 mM EDTA, 185 mM LiCl, pH 7.2","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.2,"deuteration":null},"purity_method":null,"name":"Telomere DNA G-quadruplex (2GKU) at neutral pH with LiCl","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"University of Louisville","webpage":null}],"contributor_name":"Robert","contributor_surname":"Monsen","orcid":"https://orcid.org/0000-0002-6283-3249"}],"concentration_method":null,"concentration_unit":null,"date":"2020-08-11","storage_temperature":4.0,"cell_temperature":22.0,"exposure_time":0.5,"number_of_frames":2,"wavelength":0.1033,"sample_detector_distance":3.655,"concentration_min":null,"concentration_max":4.8,"sample_volume":250.0,"flow_rate":0.6,"s_min":0.044,"s_max":3.497,"total_exposure_time":null,"seccolumn":1},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Telomere DNA G-quadruplex (2GKU) in 6 mM Li2HPO4, 2 mM LiH2PO4, 1 mM EDTA, 185 mM LiCl, pH 7.2, pH 7.2 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 4.8 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 22°C. Two successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":9.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.01666,"pddf_i0_error":null,"guinier_i0":0.0166437,"guinier_i0_error":null,"pddf_rg":1.2,"pddf_rg_error":null,"guinier_rg":1.192,"guinier_rg_error":0.016,"pddf_dmax":4.3,"pddf_dmax_error":null,"porod_volume":7.1,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":24,"guinier_point_last":367,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDW63_datloglog_img.png","symmetry":null,"last_modified":"2025-02-10T15:31:54.696467+01:00","bragg_peak":[]}