{"code":"SASDW64","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":" <scp>IgG4</scp>\n              and <scp>IgG1</scp>\n              undergo common acid‐induced compaction into an alternatively folded state","publication":{"title":" <scp>IgG4</scp>\n              and <scp>IgG1</scp>\n              undergo common acid‐induced compaction into an alternatively folded state","author_list":"Imamura H, Honda S","journal":"FEBS Letters","doi":"10.1002/1873-3468.70031","pmid":null,"published_date":"2025 Mar 17"},"status":"released","submitted_date":"2024-12-19","released_date":"2025-03-17"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDW64.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDW64.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW64_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW64_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDW64_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW64_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDW64.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 2M","resolution":0.172},"name":"Photon Factory (PF), High Energy Accelerator Research Organization (KEK)","city":"Tsukuba","country":"Japan","beamline_name":"BL-10C","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"humanized immunoglobulin G4 monoclonal antibody (lebrikizumab)","short_name":"IgG4","sequence":">SUBUNIT_1\r\nQVTLRESGPALVKPTQTLTLTCTVSGFSLSAYSVNWIRQPPGKALEWLAMIWGDGKIVYN\r\nSALKSRLTISKDTSKNQVVLTMTNMDPVDTATYYCAGDGYYPYAMDNWGQGSLVTVSSAS\r\nTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGL\r\nYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPPCPPCPAPEFLGGPSVFL\r\nFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRV\r\nVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQ\r\nVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNV\r\nFSCSVMHEALHNHYTQKSLSLSLGK\r\n>SUBUNIT_2\r\nQVTLRESGPALVKPTQTLTLTCTVSGFSLSAYSVNWIRQPPGKALEWLAMIWGDGKIVYN\r\nSALKSRLTISKDTSKNQVVLTMTNMDPVDTATYYCAGDGYYPYAMDNWGQGSLVTVSSAS\r\nTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGL\r\nYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPPCPPCPAPEFLGGPSVFL\r\nFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRV\r\nVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQ\r\nVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNV\r\nFSCSVMHEALHNHYTQKSLSLSLGK\r\n>SUBUNIT_3\r\nDIVMTQSPDSLSVSLGERATINCRASKSVDSYGNSFMHWYQQKPGQPPKLLIYLASNLES\r\nGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQNNEDPRTFGGGTKVEIKRTVAAPSVF\r\nIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLS\r\nSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC\r\n>SUBUNIT_4\r\nDIVMTQSPDSLSVSLGERATINCRASKSVDSYGNSFMHWYQQKPGQPPKLLIYLASNLES\r\nGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQNNEDPRTFGGGTKVEIKRTVAAPSVF\r\nIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLS\r\nSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC","organism":"Mouse/human","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"","mw":148.0,"total_mw":148.0,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"synthetic","molecule_description":"DrugBank ID: DB11914: https://go.drugbank.com/drugs/DB11914"}],"buffer":{"name":"10 mM sodium phosphate","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Immunoglobulin G4 (pH 7)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Toyama Prefectural University","webpage":null}],"contributor_name":"Hiroshi","contributor_surname":"Imamura","orcid":"https://orcid.org/0000-0001-8924-747X"}],"concentration_method":null,"concentration_unit":null,"date":"2024-11-19","storage_temperature":20.0,"cell_temperature":25.0,"exposure_time":2.0,"number_of_frames":5,"wavelength":0.15,"sample_detector_distance":1.0,"concentration_min":null,"concentration_max":1.2,"sample_volume":400.0,"flow_rate":0.2,"s_min":0.093,"s_max":5.662,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"Minimum q reliable is ~0.15 nm-1, approximated by a standard sample (lysozyme protein) scattering. DrugBank ID: DB11914: https://go.drugbank.com/drugs/DB11914","experiment_description":"Synchrotron SAXS data from solutions of Immunoglobulin G4 in 10 mM sodium phosphate, pH 7 were collected on the BL-10C beam line at the Photon Factory High Energy Accelerator Research Organization (KEK; Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.20 mg/ml was measured at 25°C. Five successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":146.0,"experimental_mw_error":null,"guinier_i0_mw":146.0,"guinier_i0_mw_error":2.0,"porod_mw":132.0,"porod_mw_error":null,"pddf_i0":0.1071,"pddf_i0_error":null,"guinier_i0":0.106735,"guinier_i0_error":null,"pddf_rg":4.88,"pddf_rg_error":null,"guinier_rg":4.829,"guinier_rg_error":0.708,"pddf_dmax":15.61,"pddf_dmax_error":null,"porod_volume":211.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":59,"guinier_point_last":130,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDW64_datloglog_img.png","symmetry":null,"last_modified":"2025-03-17T16:06:56.273192+01:00","bragg_peak":[]}