{"code":"SASDW84","status":"Published","type_of_curve":"Co-flow batch","angular_unit":"1/nm","project":{"title":"The CRISPR-Cas9 knockout DDC SH-SY5Y in vitro model for AADC deficiency provides insight into the pathogenicity of R347Q and L353P variants: a cross-sectional structural and functional analysis.","publication":{"title":"The CRISPR-Cas9 knockout DDC SH-SY5Y in vitro model for AADC deficiency provides insight into the pathogenicity of R347Q and L353P variants: a cross-sectional structural and functional analysis.","author_list":"Carmona-Carmona CA, Bisello G, Franchini R, Lunardi G, Galavotti R, Perduca M, Ribeiro RP, Belviso BD, Giorgetti A, Caliandro R, Lievens PM, Bertoldi M","journal":"FEBS J","doi":"10.1111/febs.70120","pmid":"40318155","published_date":"2025 May 3"},"status":"released","submitted_date":"2024-12-20","released_date":"2025-04-02"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDW84.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDW84.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW84_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW84_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDW84_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW84_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDW84.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 2M","resolution":0.172},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"point","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Aromatic-L-amino-acid decarboxylase (R347Q)","short_name":"AADC","sequence":"MNASEFRRRGKEMVDYMANYMEGIEGRQVYPDVEPGYLRPLIPAAAPQEPDTFEDIINDV\r\nEKIIMPGVTHWHSPYFFAYFPTASSYPAMLADMLCGAIGCIGFSWAASPACTELETVMMD\r\nWLGKMLELPKAFLNEKAGEGGGVIQGSASEATLVALLAARTKVIHRLQAASPELTQAAIM\r\nEKLVAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMRASALQEALERDKAAGLIPFFMV\r\nATLGTTTCCSFDNLLEVGPICNKEDIWLHVDAAYAGSAFICPEFRHLLNGVEFADSFNFN\r\nPHKWLLVNFDCSAMWVKKRTDLTGAFRLDPTYLKHSHQDSGLITDYQHWQIPLGRRFRSL\r\nKMWFVFRMYGVKGLQAYIRKHVQLSHEFESLVRQDPRFEICVEVILGLVCFRLKGSNKVN\r\nEALLQRINSAKKIHLVPCHLRDKFVLRFAICSRTVESAHVQRAWEHIKELAADVLRAERE","organism":"Homo sapiens","uniprot_code":"P20711","uniprot_range_first":1,"uniprot_range_last":478,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MNASEFRRRGKEMVDYMANYMEGIEGRQVYPDVEPGYLRPLIPAAAPQEPDTFEDIINDV\nEKIIMPGVTHWHSPYFFAYFPTASSYPAMLADMLCGAIGCIGFSWAASPACTELETVMMD\nWLGKMLELPKAFLNEKAGEGGGVIQGSASEATLVALLAARTKVIHRLQAASPELTQAAIM\nEKLVAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMRASALQEALERDKAAGLIPFFMV\nATLGTTTCCSFDNLLEVGPICNKEDIWLHVDAAYAGSAFICPEFRHLLNGVEFADSFNFN\nPHKWLLVNFDCSAMWVKKRTDLTGAFRLDPTYLKHSHQDSGLITDYRHWQIPLGRRFRSL\nKMWFVFRMYGVKGLQAYIRKHVQLSHEFESLVRQDPRFEICVEVILGLVCFRLKGSNKVN\nEALLQRINSAKKIHLVPCHLRDKFVLRFAICSRTVESAHVQRAWEHIKELAADVLRAERE","mw":53.898,"total_mw":107.796,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"50 mM HEPES, 100 µM pyridoxal 5'-phosphate","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Aromatic-L-amino-acid decarboxylase R347Q bound to pyridoxal 5'-phosphate (PLP)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"National Research Council","webpage":null}],"contributor_name":"Benny Danilo","contributor_surname":"Belviso","orcid":"https://orcid.org/0000-0003-2880-1064"}],"concentration_method":null,"concentration_unit":null,"date":"2021-07-07","storage_temperature":4.0,"cell_temperature":4.0,"exposure_time":5.0,"number_of_frames":4,"wavelength":0.1,"sample_detector_distance":2.867,"concentration_min":5.37,"concentration_max":5.37,"sample_volume":50.0,"flow_rate":0.16,"s_min":0.041,"s_max":5.174,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDW84_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"3.0.2","model_data":"https://www.sasbdb.org/media/pdb_file/SASDW84_fit1_model1.pdb","model_mw":107.27,"bead_radius":3.5,"log":"https://www.sasbdb.org/media/log_files/dammin_P1_refinement_AFfcoi5.log","symmetry":null,"comment":null,"user":930}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDW84_fit1_fixed_fit_img.png","software":"DAMMIN","chi_square_value":1.14,"p_value":0.1689,"fit_residual_plot":"SASDW84_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDW84_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/dammin_P1_refinement_YfauvID.log","software_version":"3.0.2","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"Pyridoxal 5'-phosphate: https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:18405. CAUTION: Potentially aggregated.","experiment_description":"Synchrotron SAXS data from solutions of aromatic-L-amino-acid decarboxylase (R347Q) bound to pyridoxal 5'-phosphate in 50 mM HEPES, 100 µM pyridoxal 5'-phosphate, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 5.4 and 5.4 mg/ml were measured at 4°C. Four successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":114.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":151.432,"porod_mw_error":null,"pddf_i0":548.1,"pddf_i0_error":null,"guinier_i0":564.768,"guinier_i0_error":null,"pddf_rg":3.589,"pddf_rg_error":null,"guinier_rg":3.6,"guinier_rg_error":0.02,"pddf_dmax":14.15,"pddf_dmax_error":null,"porod_volume":219.98,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":37,"guinier_point_last":54,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDW84_datloglog_img.png","symmetry":null,"last_modified":"2025-05-08T08:55:24.409452+02:00","bragg_peak":[]}