{"code":"SASDWE3","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Size-exclusion chromatography–small-angle neutron scattering system optimized for an instrument with medium neutron flux","publication":{"title":"Size-exclusion chromatography–small-angle neutron scattering system optimized for an instrument with medium neutron flux","author_list":"Morishima K, Inoue R, Nakagawa T, Shimizu M, Sakamoto R, Oda T, Mayumi K, Sugiyama M","journal":"Journal of Applied Crystallography","doi":"10.1107/S1600576725000779","pmid":null,"published_date":"2025 Feb 11"},"status":"released","submitted_date":"2024-12-09","released_date":"2025-02-12"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDWE3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDWE3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWE3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWE3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWE3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWE3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDWE3.sascif","experiment":{"instrument":{"detector":{"type":"3He position sensitive detector (PSD)","name":"ORDELA, model 2660N","resolution":5.0},"name":"JRR-3 (Japan Atomic Energy Agency)","city":"Ibaraki","country":"Japan","beamline_name":"SANS-U","beam_geometry":null,"type_of_source":"neutron source","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Circadian clock oscillator protein KaiB","short_name":"kaiB","sequence":"GPLGSMSPRKTYILKLYVAGNTPNSVRALKTLKNILEVEFQGVYALKVIDVLKNPQLAEEDKILATPTLAKVLPLPVRRIIGDLSDREKVLIGLDLLYGELQDSDDF","organism":"Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1)","uniprot_code":"Q5N595","uniprot_range_first":1,"uniprot_range_last":102,"oligomerization":"hexamer","molecular_type":"protein","uniprot_sequence":"MSPRKTYILKLYVAGNTPNSVRALKTLKNILEVEFQGVYALKVIDVLKNPQLAEEDKILA\nTPTLAKVLPLPVRRIIGDLSDREKVLIGLDLLYGELQDSDDF","mw":11.848,"total_mw":71.088,"number_molecules":6,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"The protein construct contains an additional GPLGS N-terminal extension."},{"long_name":"Circadian clock oscillator protein KaiC","short_name":"kaiC","sequence":"MASWSHPQFEKGATSAEMTSPNNNSEHQAIAKMRTMIEGFDDISHGGLPIGRSTLVSGTSGTGKTLFSIQFLYNGIIEFDEPGVFVTFEETPQDIIKNARSFGWDLAKLVDEGKLFILDASPDPEGQEVVGGFDLSALIERINYAIQKYRARRVSIDSVTSVFQQYDASSVVRRELFRLVARLKQIGATTVMTTERIEEYGPIARYGVEEFVSDNVVILRNVLEGERRRRTLEILKLRGTSHMKGEYPFTITDHGINIFPLGAMRLTQRSSNVRVSSGVVRLDEMCGGGFFKDSIILATGATGTGKTLLVSRFVENACANKERAILFAYEESRAQLLRNAYSWGMDFEEMERQNLLKIVCAYPESAGLEDHLQIIKSEINDFKPARIAIDSLSALARGVSNNAFRQFVIGVTGYAKQEEITGLFTNTSDQFMGAHSITDSHISTITDTIILLQYVEIRGEMSRAINVFKMRGSWHDKAIREFMISDKGPDIKDSFRNFERIISGSPTRITVDEKSELSRIVRGVQEKGPES","organism":"Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)","uniprot_code":"Q79PF4","uniprot_range_first":2,"uniprot_range_last":519,"oligomerization":"hexamer","molecular_type":"protein","uniprot_sequence":"MTSAEMTSPNNNSEHQAIAKMRTMIEGFDDISHGGLPIGRSTLVSGTSGTGKTLFSIQFL\nYNGIIEFDEPGVFVTFEETPQDIIKNARSFGWDLAKLVDEGKLFILDASPDPEGQEVVGG\nFDLSALIERINYAIQKYRARRVSIDSVTSVFQQYDASSVVRRELFRLVARLKQIGATTVM\nTTERIEEYGPIARYGVEEFVSDNVVILRNVLEGERRRRTLEILKLRGTSHMKGEYPFTIT\nDHGINIFPLGAMRLTQRSSNVRVSSGVVRLDEMCGGGFFKDSIILATGATGTGKTLLVSR\nFVENACANKERAILFAYEESRAQLLRNAYSWGMDFEEMERQNLLKIVCAYPESAGLEDHL\nQIIKSEINDFKPARIAIDSLSALARGVSNNAFRQFVIGVTGYAKQEEITGLFTNTSDQFM\nGAHSITDSHISTITDTIILLQYVEIRGEMSRAINVFKMRGSWHDKAIREFMISDKGPDIK\nDSFRNFERIISGSPTRITVDEKSELSRIVRGVQEKGPES","mw":59.329,"total_mw":355.974,"number_molecules":6,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"The protein construct contains an additional MASWSHPQFEKGA N-terminal extension."}],"buffer":{"name":"50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.8,"deuteration":null},"purity_method":null,"name":"Cyanobacterial circadian clock protein KaiB-KaiC complex (71% deuterated-KaiB and hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Kyoto University","webpage":null}],"contributor_name":"Ken","contributor_surname":"Morishima","orcid":"https://orcid.org/0000-0003-1091-3850"}],"concentration_method":null,"concentration_unit":null,"date":"2024-07-02","storage_temperature":10.0,"cell_temperature":25.0,"exposure_time":600.0,"number_of_frames":24,"wavelength":0.6,"sample_detector_distance":4.0,"concentration_min":null,"concentration_max":6.8,"sample_volume":500.0,"flow_rate":0.0,"s_min":0.106,"s_max":3.972,"total_exposure_time":null,"seccolumn":47},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":"SEC elution paused during SANS measurements.","experiment_description":"SANS data from solutions of the Cyanobacterial circadian clock protein KaiB-KaiC complex (71% deuterated-KaiB and hydrogenated-KaiC) in 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, 100% v/v D₂O, pH 7.8 were collected on the SANS-U beam line at JRR-3 (Japan Atomic Energy Agency; Ibaraki, Japan) using a ORDELA, model 2660N detector at a sample-detector distance of 4 and 1.03 m and at a wavelength of λ = 0.6 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Stop-flow mode in-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 500.00 μl sample at 6.8 mg/ml was injected onto a Cytiva Superdex 200 Increase 10/300 column at 25°C. 24 successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":333.0,"experimental_mw_error":null,"guinier_i0_mw":333.0,"guinier_i0_mw_error":10.0,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.536,"pddf_i0_error":null,"guinier_i0":0.546858,"guinier_i0_error":null,"pddf_rg":4.12,"pddf_rg_error":null,"guinier_rg":4.32,"guinier_rg_error":0.06,"pddf_dmax":11.7,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":5,"guinier_point_last":20,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDWE3_datloglog_img.png","symmetry":null,"last_modified":"2025-02-12T13:44:34.496707+01:00","bragg_peak":[]}