{"code":"SASDWH4","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Crystal structure of the class V GTP-binding RNA aptamer bound to its ligand: GTP recognition by a topologically complex intermolecular G-quadruplex.","publication":{"title":"Crystal structure of the class V GTP-binding RNA aptamer bound to its ligand: GTP recognition by a topologically complex intermolecular G-quadruplex.","author_list":"Stafflinger H, Neißner K, Bartsch S, Pichler AK, Bartosik K, Dhamotharan K, Abele R, Duchardt-Ferner E, Micura R, Schindelin H, Wöhnert J","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkaf1315","pmid":"41422825","published_date":"2025 Nov 26"},"status":"released","submitted_date":"2025-01-13","released_date":"2025-12-30"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDWH4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDWH4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWH4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDWH4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 6M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Class V GTP aptamer apo","short_name":null,"sequence":"GGGCGCAUUUUGGUAGGUCGGUCGCUGCUUCGGCAGUGAGGGGUAGGCAUUGCUGGCCUAGGGUGCCC","organism":"Escherichia coli","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"unknown","molecular_type":"RNA","uniprot_sequence":"","mw":22.116,"total_mw":22.116,"number_molecules":0,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"50 mM Bis-Tris, 50 mM KCl, 2 mM MgCl2","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.5,"deuteration":null},"purity_method":null,"name":"Class V GTP aptamer","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Goethe University Frankfurt, Dept. Biosciences","webpage":null}],"contributor_name":"Helena","contributor_surname":"Stafflinger","orcid":"https://orcid.org/0009-0006-6219-9942"}],"concentration_method":null,"concentration_unit":null,"date":"2024-07-19","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.095,"number_of_frames":40,"wavelength":0.123985,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":3.0,"sample_volume":null,"flow_rate":null,"s_min":0.025,"s_max":7.334,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Class V GTP aptamer in 50 mM Bis-Tris, 50 mM KCl, 2 mM MgCl2, pH 6.5 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123985 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 20°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":55.6,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":45.0,"porod_mw_error":null,"pddf_i0":0.1807,"pddf_i0_error":null,"guinier_i0":0.540714,"guinier_i0_error":null,"pddf_rg":3.365,"pddf_rg_error":null,"guinier_rg":3.27,"guinier_rg_error":0.07,"pddf_dmax":15.27,"pddf_dmax_error":null,"porod_volume":99.4,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":21,"guinier_point_last":103,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDWH4_datloglog_img.png","symmetry":null,"last_modified":"2025-12-30T13:43:06.434561+01:00","bragg_peak":[]}