{"code":"SASDWJ2","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Tangled Up in Fibers: How a Multidomain Lytic Polysaccharide Monooxygenase Binds Its Chitin Substrate.","publication":{"title":"Tangled Up in Fibers: How a Multidomain Lytic Polysaccharide Monooxygenase Binds Its Chitin Substrate.","author_list":"Sørensen HV, Montserrat-Canals M, Coder A, Prévost S, Krueger S, Vaaje-Kolstad G, Bjerregaard-Andersen K, Lund R, Krengel U","journal":"ACS Appl Mater Interfaces","doi":"10.1021/acsami.5c24418","pmid":"41709583","published_date":"2026 Feb 18"},"status":"released","submitted_date":"2024-11-19","released_date":"2025-02-09"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDWJ2.txt","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWJ2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWJ2_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWJ2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDWJ2.sascif","experiment":{"instrument":{"detector":{"type":"3-He gas detector","name":"CERCA","resolution":7.5},"name":"ILL","city":"Grenoble","country":"France","beamline_name":"D11","beam_geometry":null,"type_of_source":"neutron source","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"β-chitin nanofibers from squid pens","short_name":"β-chitin","sequence":"(C8H13O5N)n","organism":"Squid","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":null,"molecular_type":"other","uniprot_sequence":"","mw":0.203,"total_mw":0.0,"number_molecules":0,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"This is a carbohydrate polymer of varying size. Number of molecules and total kDa are not applicable."},{"long_name":"GlcNAc-binding protein A (perdeuterated)","short_name":"D-GbpA","sequence":"HGYVSAVENGVAEGRVTLCKFAANGTGEKNTHCGAIQYEPQSVEGPDGFPVTGPRDGKIASAESALAAALDEQTADRWVKRPIQAGPQTFEWTFTANHVTKDWKYYITKPNWNPNQPLSRDAFDLNPFCVVEGNMVQPPKRVSHECIVPEREGYQVILAVWDVGDTAASFYNVIDVKFDGNGPVLPDWNPAGQIIPSMDLSIGDTVYTRVFDNDGENPAYRTELKIDSETLTKANQWSYALATKINQTQKQQRAGQLNGDQFVPVYGTNPIYLKEGSGLKSVEIGYQIEAPQPEYSLTVSGLAKEYEIGEQPIQLDLTLEAQGEMSAELTVYNHHQKPLASWSQAMTDGELKSITLELSEAKAGHHMLVSRIKDRDGNLQDQQTLDFMLVEPQTPPTPGDYDFVFPNGLKEYVAGTKVLASDGAIYQCKPWPYSGYCQQWTSNATQYQPGTGSHWEMAWDKR","organism":"Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)","uniprot_code":"Q9KLD5","uniprot_range_first":24,"uniprot_range_last":485,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MKKQPKMTAIALILSGISGLAYGHGYVSAVENGVAEGRVTLCKFAANGTGEKNTHCGAIQ\nYEPQSVEGPDGFPVTGPRDGKIASAESALAAALDEQTADRWVKRPIQAGPQTFEWTFTAN\nHVTKDWKYYITKPNWNPNQPLSRDAFDLNPFCVVEGNMVQPPKRVSHECIVPEREGYQVI\nLAVWDVGDTAASFYNVIDVKFDGNGPVLPDWNPAGQIIPSMDLSIGDTVYTRVFDNDGEN\nPAYRTELKIDSETLTKANQWSYALATKINQTQKQQRAGQLNGDQFVPVYGTNPIYLKEGS\nGLKSVEIGYQIEAPQPEYSLTVSGLAKEYEIGEQPIQLDLTLEAQGEMSAELTVYNHHQK\nPLASWSQAMTDGELKSITLELSEAKAGHHMLVSRIKDRDGNLQDQQTLDFMLVEPQTPPT\nPGDYDFVFPNGLKEYVAGTKVLASDGAIYQCKPWPYSGYCQQWTSNATQYQPGTGSHWEM\nAWDKR","mw":51.253,"total_mw":51.253,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"97 % average non-exchangeable deuteration through production in E.coli cultures grown in D₂O and deuterated carbon sources."},{"long_name":"GlcNAc-binding protein A","short_name":"H-GbpA","sequence":"HGYVSAVENGVAEGRVTLCKFAANGTGEKNTHCGAIQYEPQSVEGPDGFPVTGPRDGKIASAESALAAALDEQTADRWVKRPIQAGPQTFEWTFTANHVTKDWKYYITKPNWNPNQPLSRDAFDLNPFCVVEGNMVQPPKRVSHECIVPEREGYQVILAVWDVGDTAASFYNVIDVKFDGNGPVLPDWNPAGQIIPSMDLSIGDTVYTRVFDNDGENPAYRTELKIDSETLTKANQWSYALATKINQTQKQQRAGQLNGDQFVPVYGTNPIYLKEGSGLKSVEIGYQIEAPQPEYSLTVSGLAKEYEIGEQPIQLDLTLEAQGEMSAELTVYNHHQKPLASWSQAMTDGELKSITLELSEAKAGHHMLVSRIKDRDGNLQDQQTLDFMLVEPQTPPTPGDYDFVFPNGLKEYVAGTKVLASDGAIYQCKPWPYSGYCQQWTSNATQYQPGTGSHWEMAWDKR","organism":"Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)","uniprot_code":"Q9KLD5","uniprot_range_first":24,"uniprot_range_last":485,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MKKQPKMTAIALILSGISGLAYGHGYVSAVENGVAEGRVTLCKFAANGTGEKNTHCGAIQ\nYEPQSVEGPDGFPVTGPRDGKIASAESALAAALDEQTADRWVKRPIQAGPQTFEWTFTAN\nHVTKDWKYYITKPNWNPNQPLSRDAFDLNPFCVVEGNMVQPPKRVSHECIVPEREGYQVI\nLAVWDVGDTAASFYNVIDVKFDGNGPVLPDWNPAGQIIPSMDLSIGDTVYTRVFDNDGEN\nPAYRTELKIDSETLTKANQWSYALATKINQTQKQQRAGQLNGDQFVPVYGTNPIYLKEGS\nGLKSVEIGYQIEAPQPEYSLTVSGLAKEYEIGEQPIQLDLTLEAQGEMSAELTVYNHHQK\nPLASWSQAMTDGELKSITLELSEAKAGHHMLVSRIKDRDGNLQDQQTLDFMLVEPQTPPT\nPGDYDFVFPNGLKEYVAGTKVLASDGAIYQCKPWPYSGYCQQWTSNATQYQPGTGSHWEM\nAWDKR","mw":51.253,"total_mw":51.253,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"20 mM acetate, 47% v/v D₂O","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":5.0,"deuteration":null},"purity_method":null,"name":"3 mg/mL β-chitin nanofibers from squid pens with a mixture of 0.625 mg/mL perdeuterated and 1.875 mg/mL non-deuterated N-acetylglucosamine binding Protein A (D-GbpA/H-GbpA) in 47 % D₂O","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Lund University","webpage":null}],"contributor_name":"Henrik Vinther","contributor_surname":"Sørensen","orcid":"https://orcid.org/0000-0003-3057-5668"}],"concentration_method":null,"concentration_unit":null,"date":"2020-08-18","storage_temperature":5.0,"cell_temperature":25.0,"exposure_time":900.0,"number_of_frames":1,"wavelength":0.56,"sample_detector_distance":1.7,"concentration_min":null,"concentration_max":0.63,"sample_volume":null,"flow_rate":null,"s_min":0.013,"s_max":4.1,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":"Guinier analysis and subsequent Rg, I(0) and MW parameters are not applicable for this entry as the Guinier-region occurs at a much lower s-range than the data includes. The chitin fibers are micrometers long.","experiment_description":"SANS data from solutions of β-chitin nanofibers from squid pens mixed with deuterated and non-deuterated N-acetylglucosamine binding Protein A (D-GbpA/H-GbpA) in 20 mM acetate, 47% v/v D₂O, pH 5 were collected on the D11 beam line at the Institut Laue-Langevin (ILL; Grenoble, France). Data recorded as I(s) vs s (where s = 4πsinθ/λ, and 2θ is the scattering angle), were collected a sample concentration of 3 mg/mL chitin combined with 0.625 mg/mL D-GbpA and 1.875 mg/mL H-GbpA at 25 degrees centigrade using three instrument configurations: Set-up 1: Sample-Detector distance, 1.7 m; Collimation distance, 5.5 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 900 s. Set-up 2: Sample-Detector distance, 8.0 m; Collimation distance, 8.0 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 900 s. Set-up 3: Sample-Detector distance, 38.95 m; Collimation distance, 40.0 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 900 s.","tags":[],"intensity_unit":"1/cm","experimental_mw":null,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":null,"guinier_i0_error":null,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":null,"guinier_rg_error":null,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":100,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDWJ2_datloglog_img.png","symmetry":null,"last_modified":"2026-02-23T16:32:52.341595+01:00","bragg_peak":[]}