{"code":"SASDWJ9","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.","publication":{"title":"Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.","author_list":"Tripepi A, Shakoor H, Zlobina M, Klumpler T, Kubíčková M, Houser J, Klapetek P, Lukavsky PJ","journal":"Protein Sci","doi":"10.1002/pro.70669","pmid":"42300088","published_date":"2026 Jul"},"status":"released","submitted_date":"2025-02-28","released_date":"2026-06-20"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDWJ9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDWJ9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWJ9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWJ9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWJ9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWJ9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDWJ9.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 6M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Double-stranded RNA-binding protein Staufen homolog 1 (286-577)","short_name":"dsRBD4-End","sequence":"GSHMNPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC","organism":"Homo sapiens","uniprot_code":"O95793","uniprot_range_first":286,"uniprot_range_last":577,"oligomerization":"other","molecular_type":"protein","uniprot_sequence":"MSQVQVQVQNPSAALSGSQILNKNQSLLSQPLMSIPSTTSSLPSENAGRPIQNSALPSAS\nITSTSAAAESITPTVELNALCMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPF\nPVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESEEEN\nLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKN\nAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKE\nKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQ\nPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPM\nVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGH\nVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVES\nCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC","mw":32.432,"total_mw":32.432,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"Monomer - dimer mixture. Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated RNA-binding domain 3. The protein construct used for SAXS contains an additional four non-native amino acids at the N-terminus (GSHM)."}],"buffer":{"name":"50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated RNA-binding domain 3","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"CEITEC - Central European Institute of Technology, Masaryk University","webpage":"https://www.ceitec.eu/"}],"contributor_name":"Tomas","contributor_surname":"Klumpler","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2021-04-23","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":63,"wavelength":0.123983,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":7.4,"sample_volume":100.0,"flow_rate":0.7,"s_min":0.138,"s_max":4.0,"total_exposure_time":null,"seccolumn":1},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDWJ9_fit1_model1_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":"ATSAS 2.8.4","model_data":"https://www.sasbdb.org/media/pdb_file/SASDWJ9_fit1_model1.pdb","model_mw":32.69,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_QQgalKk.log","symmetry":null,"comment":null,"user":195},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDWJ9_fit1_model2_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":"ATSAS 2.8.4","model_data":"https://www.sasbdb.org/media/pdb_file/SASDWJ9_fit1_model2.pdb","model_mw":63.94,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_s8kiVzs.log","symmetry":null,"comment":null,"user":195}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDWJ9_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":1.091,"p_value":0.2827,"fit_residual_plot":"SASDWJ9_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDWJ9_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_3uRHhol.log","software_version":"ATSAS 2.8.4","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated RNA-binding domain 3 in 50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123983 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 7.4 mg/ml was injected at a 0.70 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 63 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":14.8,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":28.7,"porod_mw_error":null,"pddf_i0":79.39,"pddf_i0_error":null,"guinier_i0":80.1449,"guinier_i0_error":null,"pddf_rg":4.11,"pddf_rg_error":null,"guinier_rg":4.026,"guinier_rg_error":0.027,"pddf_dmax":12.85,"pddf_dmax_error":null,"porod_volume":45.9,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":5,"guinier_point_last":67,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDWJ9_datloglog_img.png","symmetry":null,"last_modified":"2026-06-20T15:51:41.524220+02:00","bragg_peak":[]}