{"code":"SASDWR5","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"A protein-dependent riboswitch activates ribosomal frameshifting in cardioviruses","publication":{"title":"A protein-dependent riboswitch activates ribosomal frameshifting in cardioviruses","author_list":"Betts J, Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C","journal":"Molecular Cell","doi":"10.1016/j.molcel.2026.07.036","pmid":null,"published_date":"2026 Aug"},"status":"released","submitted_date":"2025-04-01","released_date":"2025-07-20"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDWR5.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDWR5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWR5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWR5_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWR5_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWR5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDWR5.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 6M","resolution":null},"name":"PETRA III","city":"DESY; 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Middle: a MONSA ab initio dummy-atom model reconstruction of the RNA particle. Bottom: A pseudoknot conformation based on the X-ray crystal structure of the RNA in complex with Protein 2A (refer to SASBDB entry SASDWS5; https://www.sasbdb.org/data/SASDWS5). The full entry archive contains the unsubtracted SEC-SAXS 1D-data frames in addition to DAMMIN, MONSA and SREFLEX modelling routines.","experiment_description":"Synchrotron SAXS data from solutions of stimulatory element RNA in 20 mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123983 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 7.1 mg/ml was injected at a 0.35 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 20°C. 57 successive 0.250 second frames were collected through the SEC-elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":13.5,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":10.0,"porod_mw_error":null,"pddf_i0":7577.0,"pddf_i0_error":null,"guinier_i0":7530.62,"guinier_i0_error":null,"pddf_rg":1.989,"pddf_rg_error":null,"guinier_rg":1.922,"guinier_rg_error":0.003,"pddf_dmax":8.0,"pddf_dmax_error":null,"porod_volume":18.9,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":49,"guinier_point_last":221,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDWR5_datloglog_img.png","symmetry":null,"last_modified":"2026-08-21T18:54:38.194802+02:00","bragg_peak":[]}