{"code":"SASDWS3","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"The <i>Paulinella</i>\n              chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold","publication":{"title":"The <i>Paulinella</i>\n              chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold","author_list":"Klimenko V, Reiners J, Applegate V, Reimann K, Popowicz G, Hoeppner A, Papadopoulos A, Smits S, Nowack E","journal":"Plant Physiology","doi":"10.1093/plphys/kiaf504","pmid":null,"published_date":"2025 Oct 10"},"status":"released","submitted_date":"2024-12-04","released_date":"2025-10-13"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDWS3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDWS3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWS3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWS3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWS3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWS3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDWS3.sascif","experiment":{"instrument":{"detector":{"type":"pixel counting","name":"Pilatus3 R 300K","resolution":172.0},"name":"Center for Structural Studies, Heinrich-Heine-University","city":"Düsseldorf","country":"Germany","beamline_name":"Xenocs Xeuss 2.0 Q-Xoom","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"N-acetyl-gamma-glutamyl-phosphate reductase (ArgC)","short_name":"crTPpart2_ArgC+ArgC","sequence":"GFSADGAPTQPFVPRKGIDKFVVRPAPVGPFQLVSPGVSEPSTLFLYGEDAYEGEEAWLYGVKLTAEVAVPTGVPGDVLKGKLLRWPSSSVKEKLKAADETYMKEGVKRGVVSVVLQDGSPEQAYWYFQFHKSKPSPKVFIDGEAGTTGLQVRERLSKRCDLELISLTNELRKDPAARRAALNEADVAILCLPDDAAKEAVSLIENDHTVVIDASTAYRVAEGWTYGFPELTPTQAKSLAASKRISNPGCYPTGFISLIRPLVDAGFIPPTAGITVNAVSGYTGGGKALIRIYEEENAEPWGAYGFNLAHKHTPEMAKWSGLTREPIFQPSVGSFPQGMVVSVPLHYDQLNAAGRSGAALHKCLAERYAGSSFVSVRPLNKMDDLERGAFMRPDTLANSNELELSVYANDAKQTAWLVARLDNLGKGASGAAVQNLNLVLGLDERAGLC","organism":"Paulinella chromatophora","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"","mw":48.37,"total_mw":96.74,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"No UniProt ID is available so far.\r\nThis sequence was derived from the transcripts (GenBank: GEZN00000000.1,  Nowack, E. C. M., Price, D. C., Bhattacharya, D., Singer, A., Melkonian, M. and Grossman, A. R. (2016) Gene transfers from diverse bacteria compensate for reductive genome evolution in the chromatophore of Paulinella chromatophora. Proc. Nati. Acad. Sci. USA, 113, 12214-12219.) \r\n\r\nscaffold7023-size1590, encoding a protein annotated as N-acetyl-gamma-glutamyl-phosphate reductase (ArgC)"}],"buffer":{"name":"20 mM HEPES, 300 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"N-acetyl-gamma-glutamyl-phosphate reductase (ArgC)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Düsseldorf, Germany","full_name":"Heinrich Heine University Düsseldorf","webpage":"https://www.uni-duesseldorf.de"}],"contributor_name":"Jens","contributor_surname":"Reiners","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2023-03-10","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":600.0,"number_of_frames":18,"wavelength":0.154,"sample_detector_distance":0.55,"concentration_min":1.16,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.14,"s_max":4.34,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDWS3_fit1_model1_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":"GAJOE - version 2.1 - (r14885)","model_data":"https://www.sasbdb.org/media/pdb_file/SASDWS3_fit1_model1.pdb","model_mw":96.97,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_lOFfMxl.log","symmetry":"P1","comment":"Volume fraction (6/9)","user":402},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDWS3_fit1_model2_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":"GAJOE - version 2.1 - (r14885)","model_data":"https://www.sasbdb.org/media/pdb_file/SASDWS3_fit1_model2.pdb","model_mw":96.97,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_UTirQOV.log","symmetry":"P1","comment":"Volume fraction (2/9)","user":402},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDWS3_fit1_model3_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":"GAJOE - version 2.1 - (r14885)","model_data":"https://www.sasbdb.org/media/pdb_file/SASDWS3_fit1_model3.pdb","model_mw":96.97,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_5RgsWT4.log","symmetry":"P1","comment":"Volume fraction (1/9)","user":402}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDWS3_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":1.179,"p_value":0.1596,"fit_residual_plot":"SASDWS3_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDWS3_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_O2nfGRG.log","software_version":"GAJOE - version 2.1 - (r14885)","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of ArgC in 20 mM HEPES, 300 mM NaCl, pH 8 were collected on a Xenocs Xeuss 2.0 Q-Xoom instrument at the Center for Structural Studies, Heinrich-Heine-University (Düsseldorf, Germany) using a Pilatus3 R 300K detector at a sample-detector distance of 0.6 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.2 and 10 mg/ml were measured at 10°C. 18 successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":96.33,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":106.0,"porod_mw_error":null,"pddf_i0":0.06443,"pddf_i0_error":null,"guinier_i0":0.0647917,"guinier_i0_error":null,"pddf_rg":3.613,"pddf_rg_error":null,"guinier_rg":3.604,"guinier_rg_error":0.017,"pddf_dmax":13.11,"pddf_dmax_error":null,"porod_volume":170.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":39,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDWS3_datloglog_img.png","symmetry":null,"last_modified":"2025-10-13T13:58:35.923755+02:00","bragg_peak":[]}