{"code":"SASDWW3","status":"Published","type_of_curve":"Extrapolated to infinite dilution","angular_unit":"1/nm","project":{"title":"The <i>Paulinella</i>\n              chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold","publication":{"title":"The <i>Paulinella</i>\n              chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold","author_list":"Klimenko V, Reiners J, Applegate V, Reimann K, Popowicz G, Hoeppner A, Papadopoulos A, Smits S, Nowack E","journal":"Plant Physiology","doi":"10.1093/plphys/kiaf504","pmid":null,"published_date":"2025 Oct 10"},"status":"released","submitted_date":"2024-12-04","released_date":"2025-10-13"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDWW3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDWW3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWW3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWW3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWW3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWW3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDWW3.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 6M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"crTPpart2 of cysteine synthase A (CysK)","short_name":"crTPpart2_CysK","sequence":"GATSSSISAGGAAGADSSFIPKIGVNAVVARPNPPGPLQLLPPGISSPATLFIYPSMESLLGKDFGNGATSESAWVYGAKLTSDEAGALPVLTGQPSDVLKGRLVSFPPMGFPSKLRLVDGLMNFGGLSTTLREMVPVVKKDGSTIDTYWYYQ","organism":"Paulinella chromatophora","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"","mw":15.821,"total_mw":15.821,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"No UniProt ID is available. This sequence was derived from the transcripts (GenBank: GEZN00000000.1,  Nowack, E. C. M., Price, D. C., Bhattacharya, D., Singer, A., Melkonian, M. and Grossman, A. R. (2016) Gene transfers from diverse bacteria compensate for reductive genome evolution in the chromatophore of Paulinella chromatophora. Proc. Nati. Acad. Sci. USA, 113, 12214-12219.) Scaffold4337-size1989, encoding a protein annotated as cysteine synthase A (CysK)."}],"buffer":{"name":"20 mM HEPES, 300 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"crTPpart2 of cysteine synthase A (CysK)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Düsseldorf, Germany","full_name":"Heinrich Heine University Düsseldorf","webpage":"https://www.uni-duesseldorf.de"}],"contributor_name":"Jens","contributor_surname":"Reiners","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2022-09-14","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":0.095,"number_of_frames":40,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":0.7,"concentration_max":10.6,"sample_volume":null,"flow_rate":null,"s_min":0.321,"s_max":4.089,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of crTPpart2 of CysK in 20 mM HEPES, 300 mM NaCl, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.7 and 10.6 mg/ml were measured at 10°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.","tags":[],"intensity_unit":"1/cm","experimental_mw":13.69,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":17.7,"porod_mw_error":null,"pddf_i0":0.008933,"pddf_i0_error":null,"guinier_i0":0.00893092,"guinier_i0_error":null,"pddf_rg":1.672,"pddf_rg_error":null,"guinier_rg":1.65,"guinier_rg_error":0.004,"pddf_dmax":6.17,"pddf_dmax_error":null,"porod_volume":28.34,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":168,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDWW3_datloglog_img.png","symmetry":null,"last_modified":"2025-10-13T13:58:55.484517+02:00","bragg_peak":[]}