{"code":"SASDX42","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Biochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the Fab' region of IgA1 and IgA2.","publication":{"title":"Biochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the Fab' region of IgA1 and IgA2.","author_list":"Ramírez-Larrota JS, Juyoux P, Guerra P, Eckhard U, Gomis-Rüth FX","journal":"PLoS Pathog","doi":"10.1371/journal.ppat.1013292","pmid":"40627637","published_date":"2025 Jul"},"status":"released","submitted_date":"2025-02-27","released_date":"2025-06-18"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDX42.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDX42.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX42_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX42_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDX42_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX42_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDX42.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 2M","resolution":0.172},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"point","type_of_source":"X-ray 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(E540A)","short_name":"IgAse","sequence":"MASKPDIKVGDYVKMGVYNNASILWRCVSIDNNGPLMLADKIVDTLAYDAKTNDNSNSKSHSRSYKRDDY\r\nGSNYWKDSNMRSWLNSTAAEGKVDWLCGNPPKDGYVSGVGAYNEKAGFLNAFSKSEIAAMKTVTQRSLVS\r\nHPEYNKGIVDGDANSDLLYYTDISEAVANYDSSYFETTTEKVFLLDVKQANAVWKNLKGYYVAYNNDGMA\r\nWPYWLRTPVTDCNHDMRYISSSGQVGRYAPWYSDLGVRPAFYLDSEYFVTTSGSGSQSSPYIGSAPNKQE\r\nDDYTISEPAEDANPDWNVSTEQSIQLTLGPWYSNDGKYSNPTIPVYTIQKTRSDTENMVVVVCGEGYTKS\r\nQQGKFINDVKRLWQDAMKYEPYRSYADRFNVYALCTASESTFDNGGSTFFDVIVDKYNSPVISNNLHGSQ\r\nWKNHIFERCIGPEFIEKIHDAHIKKKCDPNTIPSGSEYEPYYYVHDYIAQFAMVVNTKSDFGGAYNNREY\r\nGFHYFISPSDSYRASKTFAHAFGHGLLGLGDEYSNGYLLDDKELKSLNLSSVEDPEKIKWRQLLGFRNTY\r\nTCRNAYGSKMLVSSYECIMRDTNYQFCEVCRLQGFKRMSQLVKDVDLYVATPEVKEYTGAYSKPSDFTDL\r\nETSSYYNYTYNRNDRLLSGNSKSRFNTNMNGKKIELRTVIQNISDKNARQLKFKMWIKHSDGSVATDSSG\r\nNPLQTVQTFDIPVWNDKANFWPLGALDHIKSDFNSGLKSCSLIYQIPSDAQLKSGDTVAFQVLDENGNVL\r\nADDNTETQRYTTVSIQYKFEDGSEIPNTAGGTFTVPYGTKLDLTPAKTLYDYEFIKVDGLNKPIVSDGTV\r\nVTYYYKNKNEEHTHNLTLVAAKAATCTTAGNSAYYTCDGCDKWFADATGSVEITDKTSVKIPAPGHTAGT\r\nEWKSDDTNHWHECTVAGCGVIIESTKSAHTAGEWIVDTPATATTAGTKHKECTVCHRVLETQPIPSTGTE\r\nLKIIAGDNQIYNKASGSDVTITCNGDFAKFTGIKVDGSVVDSSNYTAVSGSTVLTLKASYLGTLTDGSHT\r\nITFVYTDGEANANLTVRTAGSGHIHDYGTEWKSNADNHWHECNCGDKKDEAAHSFKWVVDKEATATKKGS\r\nKHEECKICGYKRSAVEILEHHHHHH","organism":"Thomasclavelia ramosa","uniprot_code":"Q9AES2","uniprot_range_first":31,"uniprot_range_last":1166,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MTKKLMTKKITAIFLALYMAISVLPMTIQAASKPDIKVGDYVKMGVYNNASILWRCVSID\nNNGPLMLADKIVDTLAYDAKTNDNSNSKSHSRSYKRDDYGSNYWKDSNMRSWLNSTAAEG\nKVDWLCGNPPKDGYVSGVGAYNEKAGFLNAFSKSEIAAMKTVTQRSLVSHPEYNKGIVDG\nDANSDLLYYTDISEAVANYDSSYFETTTEKVFLLDVKQANAVWKNLKGYYVAYNNDGMAW\nPYWLRTPVTDCNHDMRYISSSGQVGRYAPWYSDLGVRPAFYLDSEYFVTTSGSGSQSSPY\nIGSAPNKQEDDYTISEPAEDANPDWNVSTEQSIQLTLGPWYSNDGKYSNPTIPVYTIQKT\nRSDTENMVVVVCGEGYTKSQQGKFINDVKRLWQDAMKYEPYRSYADRFNVYALCTASEST\nFDNGGSTFFDVIVDKYNSPVISNNLHGSQWKNHIFERCIGPEFIEKIHDAHIKKKCDPNT\nIPSGSEYEPYYYVHDYIAQFAMVVNTKSDFGGAYNNREYGFHYFISPSDSYRASKTFAHE\nFGHGLLGLGDEYSNGYLLDDKELKSLNLSSVEDPEKIKWRQLLGFRNTYTCRNAYGSKML\nVSSYECIMRDTNYQFCEVCRLQGFKRMSQLVKDVDLYVATPEVKEYTGAYSKPSDFTDLE\nTSSYYNYTYNRNDRLLSGNSKSRFNTNMNGKKIELRTVIQNISDKNARQLKFKMWIKHSD\nGSVATDSSGNPLQTVQTFDIPVWNDKANFWPLGALDHIKSDFNSGLKSCSLIYQIPSDAQ\nLKSGDTVAFQVLDENGNVLADDNTETQRYTTVSIQYKFEDGSEIPNTAGGTFTVPYGTKL\nDLTPAKTLYDYEFIKVDGLNKPIVSDGTVVTYYYKNKNEEHTHNLTLVAAKAATCTTAGN\nSAYYTCDGCDKWFADATGSVEITDKTSVKIPAPGHTAGTEWKSDDTNHWHECTVAGCGVI\nIESTKSAHTAGEWIVDTPATATTAGTKHKECTVCHRVLETQPIPSTGTELKIIAGDNQIY\nNKASGSDVTITCNGDFAKFTGIKVDGSVVDSSNYTAVSGSTVLTLKASYLGTLTDGSHTI\nTFVYTDGEANANLTVRTAGSGHIHDYGTEWKSNADNHWHECNCGDKKDEAAHSFKWVVDK\nEATATKKGSKHEECKICGYKRSAVEIPATGTSTAPTDTTKPNDTTKPGNTNGSEKSPQTG\nDNSNIFLWFALLFVSAAGVTGITAYNKKKKEHAE","mw":127.857,"total_mw":127.857,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"The protein construct used for SAXS contains an additional C-terminal polyhistidine tag (LEHHHHHH)."}],"buffer":{"name":"50 mM Tris, 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"IgA peptidase (A31-I1166; active site mutant E540A)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Molecular Biology Institute of Barcelona","webpage":null}],"contributor_name":"Juan Sebastián","contributor_surname":"Ramírez Larrota","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2023-05-23","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":4.0,"number_of_frames":250,"wavelength":0.099,"sample_detector_distance":2.867,"concentration_min":null,"concentration_max":4.0,"sample_volume":null,"flow_rate":null,"s_min":0.063,"s_max":5.21,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDX42_fit1_model1_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDX42_fit1_model1.cif","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":null,"comment":"0.44 volume fraction","user":1196},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDX42_fit1_model2_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDX42_fit1_model2.cif","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":null,"comment":"0.11 volume fraction","user":1196},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDX42_fit1_model3_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDX42_fit1_model3.cif","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":null,"comment":"0.11 volume fraction","user":1196},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDX42_fit1_model4_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDX42_fit1_model4.cif","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":null,"comment":"0.22 volume fraction","user":1196},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDX42_fit1_model5_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDX42_fit1_model5.cif","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":null,"comment":"0.11 volume fraction","user":1196}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDX42_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":1.541,"p_value":0.0139,"fit_residual_plot":"SASDX42_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDX42_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_9MVb3vS.log","software_version":"2.1","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The results obtained from EOM analysis are made available in the full entry zip archive.","experiment_description":"Synchrotron SAXS data from solutions of IgA peptidase in 50 mM Tris, 150 mM NaCl, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 20°C. 250 successive 4 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":118.8,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":119.0,"porod_mw_error":null,"pddf_i0":72.97,"pddf_i0_error":null,"guinier_i0":70.169,"guinier_i0_error":null,"pddf_rg":6.433,"pddf_rg_error":null,"guinier_rg":5.65,"guinier_rg_error":0.035,"pddf_dmax":27.9,"pddf_dmax_error":null,"porod_volume":190.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":6,"guinier_point_last":33,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDX42_datloglog_img.png","symmetry":null,"last_modified":"2025-07-21T18:12:24.155654+02:00","bragg_peak":[]}