{"code":"SASDX64","status":"Published","type_of_curve":"Co-flow SEC-SAS","angular_unit":"1/A","project":{"title":"Unique structural and ligand-binding properties of the <i>Staphylococcus aureus</i>\n              serine hydrolase FphE","publication":{"title":"Unique structural and ligand-binding properties of the <i>Staphylococcus aureus</i>\n              serine hydrolase FphE","author_list":"Jo J, Upadhyay T, You X, Bennett J, Lee H, Bogyo M, Fellner M","journal":"Proceedings of the National Academy of Sciences","doi":"10.1073/pnas.2532683123","pmid":null,"published_date":"2026 Mar 24"},"status":"released","submitted_date":"2025-05-02","released_date":"2026-03-24"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDX64.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDX64.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX64_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX64_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDX64_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX64_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDX64.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 2M","resolution":172.0},"name":"Australian Synchrotron","city":"Melbourne","country":"Australia","beamline_name":"BioSAXS","beam_geometry":null,"type_of_source":"X-ray 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FphE","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"University of Otago","webpage":null}],"contributor_name":"XIANGYAN","contributor_surname":"YOU","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2025-03-26","storage_temperature":21.0,"cell_temperature":21.0,"exposure_time":1.0,"number_of_frames":641,"wavelength":0.1,"sample_detector_distance":2.5,"concentration_min":null,"concentration_max":1.0,"sample_volume":50.0,"flow_rate":0.4,"s_min":0.05,"s_max":5.517,"total_exposure_time":null,"seccolumn":4},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDX64_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDX64_fit1_model1.cif","model_mw":null,"bead_radius":null,"log":null,"symmetry":"P2","comment":null,"user":1216}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDX64_fit1_fixed_fit_img.png","software":"DAMMIF","chi_square_value":1.025,"p_value":0.1198,"fit_residual_plot":"SASDX64_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDX64_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/2P_E_1P0_5_8.log","software_version":null,"description":"Symmetery 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HEPES, 50 mM NaCl, 0.1% w/v NaN3, pH 7.5 were collected on the BioSAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus3 X 2M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Co-flow in-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 1.0 mg/ml was injected at a 0.40 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 21°C. 641 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":56.8,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":52.0,"porod_mw_error":null,"pddf_i0":0.002924,"pddf_i0_error":null,"guinier_i0":0.00291592,"guinier_i0_error":null,"pddf_rg":3.07,"pddf_rg_error":null,"guinier_rg":3.008,"guinier_rg_error":0.361,"pddf_dmax":10.0,"pddf_dmax_error":null,"porod_volume":84.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":9,"guinier_point_last":70,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDX64_datloglog_img.png","symmetry":null,"last_modified":"2026-03-25T10:08:21.394452+01:00","bragg_peak":[]}