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Co-expression with DnaK-DnaJ-GrpE (pKJE7) significantly improved solubility, but the protein did not bind to Ni-NTA resin. Downstream characterization confirmed that recombinant HDPpf is in complex with DnaK (DnaK-HDPpf)"}],"buffer":{"name":"20 mM Tris-Cl, 200 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Chaperone protein DnaK (E.coli) & recombinant Heme ligase (HDP) (P.fulciparum) protein complex","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Mumbai 400085, INDIA","full_name":"Bhabha Atomic Research Centre","webpage":null}],"contributor_name":"Dr. Rahul","contributor_surname":"Singh","orcid":"https://orcid.org/0000-0003-4871-3600"}],"concentration_method":null,"concentration_unit":null,"date":"2019-02-26","storage_temperature":4.0,"cell_temperature":25.0,"exposure_time":30.0,"number_of_frames":100,"wavelength":1.5406,"sample_detector_distance":null,"concentration_min":null,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.11,"s_max":4.0,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDXF5_fit1_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"GASBOR 2.3i (r14885)","model_data":"https://www.sasbdb.org/media/pdb_file/SASDXF5_fit1_model1.cif","model_mw":null,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/DnaK-HDP_6dBQCQj.fit","symmetry":"NA","comment":null,"user":649},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDXF5_fit1_model2_img.png","software":"AlphaFold","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":"AlphaFold 3","model_data":"https://www.sasbdb.org/media/pdb_file/SASDXF5_fit1_model2.pdb","model_mw":82.37,"bead_radius":null,"log":null,"symmetry":"NA","comment":"The PDB structure of DnaK (7KZI) is modeled with HDPpf (AlphaFold; Q8IL04) using the ab-initio Gasbo","user":649}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDXF5_fit1_fixed_fit_img.png","software":"GASBOR","chi_square_value":35.33,"p_value":0.0,"fit_residual_plot":"SASDXF5_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDXF5_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/DnaK-HDP.log","software_version":"GASBOR 2.3i (r14885)","description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDXF5_fit2_model1_img.png","software":"AlphaFold","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":"AlphaFold 3","model_data":"https://www.sasbdb.org/media/pdb_file/SASDXF5_fit2_model1.pdb","model_mw":82.37,"bead_radius":null,"log":null,"symmetry":"NA","comment":"The PDB structure of DnaK (7KZI) is modeled with HDPpf (AlphaFold; Q8IL04) using the ab-initio Gasbo","user":649}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDXF5_fit2_fixed_fit_img.png","software":"CRYSOL","chi_square_value":48.441,"p_value":0.0,"fit_residual_plot":"SASDXF5_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDXF5_fit2.fit","fit_log":null,"software_version":"ATSAS 3.3.0 (r14945)","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":70.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":51.0,"porod_mw_error":null,"pddf_i0":0.2824,"pddf_i0_error":null,"guinier_i0":0.276735,"guinier_i0_error":null,"pddf_rg":4.06,"pddf_rg_error":null,"guinier_rg":3.828,"guinier_rg_error":0.108,"pddf_dmax":13.8,"pddf_dmax_error":null,"porod_volume":83.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":8,"guinier_point_last":67,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDXF5_datloglog_img.png","symmetry":null,"last_modified":"2025-08-13T10:03:08.381002+02:00","bragg_peak":[]}